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Median-Based Absolute Quantification of Proteins Using Fully Unlabeled Generic Internal Standard (FUGIS)

[Image: see text] By reporting the molar abundance of proteins, absolute quantification determines their stoichiometry in complexes, pathways, or networks. Typically, absolute quantification relies either on protein-specific isotopically labeled peptide standards or on a semiempirical calibration ag...

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Detalles Bibliográficos
Autores principales: Raghuraman, Bharath Kumar, Bogdanova, Aliona, Moon, HongKee, Rzagalinski, Ignacy, Geertsma, Eric R., Hersemann, Lena, Shevchenko, Andrej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8749952/
https://www.ncbi.nlm.nih.gov/pubmed/34807614
http://dx.doi.org/10.1021/acs.jproteome.1c00596

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