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Evaluation of Changes to the Oral Microbiome Based on 16S rRNA Sequencing among Children Treated for Cancer

SIMPLE SUMMARY: Childhood cancer survivors suffer from many oral complications during and after primary therapy. Our study focuses on changes in the oral microbiome of cancer survivors. Using 16S rRNA sequencing, we observed global and distinct changes in oral microbiome associated with a patient’s...

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Autores principales: Proc, Patrycja, Szczepańska, Joanna, Zarzycka, Beata, Szybka, Małgorzata, Borowiec, Maciej, Płoszaj, Tomasz, Fendler, Wojciech, Chrzanowski, Jędrzej, Zubowska, Małgorzata, Stolarska, Małgorzata, Młynarski, Wojciech
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8750156/
https://www.ncbi.nlm.nih.gov/pubmed/35008173
http://dx.doi.org/10.3390/cancers14010007
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author Proc, Patrycja
Szczepańska, Joanna
Zarzycka, Beata
Szybka, Małgorzata
Borowiec, Maciej
Płoszaj, Tomasz
Fendler, Wojciech
Chrzanowski, Jędrzej
Zubowska, Małgorzata
Stolarska, Małgorzata
Młynarski, Wojciech
author_facet Proc, Patrycja
Szczepańska, Joanna
Zarzycka, Beata
Szybka, Małgorzata
Borowiec, Maciej
Płoszaj, Tomasz
Fendler, Wojciech
Chrzanowski, Jędrzej
Zubowska, Małgorzata
Stolarska, Małgorzata
Młynarski, Wojciech
author_sort Proc, Patrycja
collection PubMed
description SIMPLE SUMMARY: Childhood cancer survivors suffer from many oral complications during and after primary therapy. Our study focuses on changes in the oral microbiome of cancer survivors. Using 16S rRNA sequencing, we observed global and distinct changes in oral microbiome associated with a patient’s age and therapy duration, but not antibiotic therapy or cancer type. Observed changes in the oral microbiome could differentiate patients at higher risk of long-term oral complications. ABSTRACT: A child’s mouth is the gateway to many species of bacteria. Changes in the oral microbiome may affect the health of the entire body. The aim of the study was to evaluate the changes in the oral microbiome of childhood cancer survivors. Saliva samples before and after anti-cancer treatment were collected from 20 patients aged 6–18 years, diagnosed de novo with cancer in 2018–2019 (7 girls and 13 boys, 7.5–19 years old at the second time point). Bacterial DNA was extracted, and the microbial community profiles were assessed by 16S rRNA sequencing. The relative abundances of Cellulosilyticum and Tannerella genera were found to significantly change throughout therapy (p = 0.043 and p = 0.036, respectively). However, no differences in the alpha-diversity were observed (p = 0.817). The unsupervised classification revealed two clusters of patients: the first with significant changes in Campylobacter and Fusobacterium abundance, and the other with change in Neisseria. These two groups of patients differed in median age (10.25 vs. 16.16 years; p = 0.004) and the length of anti-cancer therapy (19 vs. 4 months; p = 0.003), but not cancer type or antibiotic treatment.
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spelling pubmed-87501562022-01-12 Evaluation of Changes to the Oral Microbiome Based on 16S rRNA Sequencing among Children Treated for Cancer Proc, Patrycja Szczepańska, Joanna Zarzycka, Beata Szybka, Małgorzata Borowiec, Maciej Płoszaj, Tomasz Fendler, Wojciech Chrzanowski, Jędrzej Zubowska, Małgorzata Stolarska, Małgorzata Młynarski, Wojciech Cancers (Basel) Article SIMPLE SUMMARY: Childhood cancer survivors suffer from many oral complications during and after primary therapy. Our study focuses on changes in the oral microbiome of cancer survivors. Using 16S rRNA sequencing, we observed global and distinct changes in oral microbiome associated with a patient’s age and therapy duration, but not antibiotic therapy or cancer type. Observed changes in the oral microbiome could differentiate patients at higher risk of long-term oral complications. ABSTRACT: A child’s mouth is the gateway to many species of bacteria. Changes in the oral microbiome may affect the health of the entire body. The aim of the study was to evaluate the changes in the oral microbiome of childhood cancer survivors. Saliva samples before and after anti-cancer treatment were collected from 20 patients aged 6–18 years, diagnosed de novo with cancer in 2018–2019 (7 girls and 13 boys, 7.5–19 years old at the second time point). Bacterial DNA was extracted, and the microbial community profiles were assessed by 16S rRNA sequencing. The relative abundances of Cellulosilyticum and Tannerella genera were found to significantly change throughout therapy (p = 0.043 and p = 0.036, respectively). However, no differences in the alpha-diversity were observed (p = 0.817). The unsupervised classification revealed two clusters of patients: the first with significant changes in Campylobacter and Fusobacterium abundance, and the other with change in Neisseria. These two groups of patients differed in median age (10.25 vs. 16.16 years; p = 0.004) and the length of anti-cancer therapy (19 vs. 4 months; p = 0.003), but not cancer type or antibiotic treatment. MDPI 2021-12-21 /pmc/articles/PMC8750156/ /pubmed/35008173 http://dx.doi.org/10.3390/cancers14010007 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Proc, Patrycja
Szczepańska, Joanna
Zarzycka, Beata
Szybka, Małgorzata
Borowiec, Maciej
Płoszaj, Tomasz
Fendler, Wojciech
Chrzanowski, Jędrzej
Zubowska, Małgorzata
Stolarska, Małgorzata
Młynarski, Wojciech
Evaluation of Changes to the Oral Microbiome Based on 16S rRNA Sequencing among Children Treated for Cancer
title Evaluation of Changes to the Oral Microbiome Based on 16S rRNA Sequencing among Children Treated for Cancer
title_full Evaluation of Changes to the Oral Microbiome Based on 16S rRNA Sequencing among Children Treated for Cancer
title_fullStr Evaluation of Changes to the Oral Microbiome Based on 16S rRNA Sequencing among Children Treated for Cancer
title_full_unstemmed Evaluation of Changes to the Oral Microbiome Based on 16S rRNA Sequencing among Children Treated for Cancer
title_short Evaluation of Changes to the Oral Microbiome Based on 16S rRNA Sequencing among Children Treated for Cancer
title_sort evaluation of changes to the oral microbiome based on 16s rrna sequencing among children treated for cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8750156/
https://www.ncbi.nlm.nih.gov/pubmed/35008173
http://dx.doi.org/10.3390/cancers14010007
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