Cargando…

Compositional and Functional Characteristics of Feta-Type Cheese Made from Micellar Casein Concentrate

Micellar casein concentrate (MCC) is a high protein ingredient (obtained by microfiltration of skim milk) with an elevated level of casein as a percentage of total protein (TP) compared to skim milk. It can be used as an ingredient in cheese making. Feta-type cheese is a brined soft cheese with a sa...

Descripción completa

Detalles Bibliográficos
Autores principales: Hammam, Ahmed R. A., Kapoor, Rohit, Salunke, Prafulla, Metzger, Lloyd E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8750234/
https://www.ncbi.nlm.nih.gov/pubmed/35010150
http://dx.doi.org/10.3390/foods11010024
_version_ 1784631414722920448
author Hammam, Ahmed R. A.
Kapoor, Rohit
Salunke, Prafulla
Metzger, Lloyd E.
author_facet Hammam, Ahmed R. A.
Kapoor, Rohit
Salunke, Prafulla
Metzger, Lloyd E.
author_sort Hammam, Ahmed R. A.
collection PubMed
description Micellar casein concentrate (MCC) is a high protein ingredient (obtained by microfiltration of skim milk) with an elevated level of casein as a percentage of total protein (TP) compared to skim milk. It can be used as an ingredient in cheese making. Feta-type cheese is a brined soft cheese with a salty taste and acid flavor. We theorize that Feta-type cheese can be produced from MCC instead of milk, which can improve the efficiency of manufacture and allow for the removal of whey proteins before manufacturing Feta-type cheese. The objectives of this study were to develop a process of producing Feta-type cheese from MCC and to determine the optimum protein content in MCC to make Feta-type cheese. MCC solutions with 3% (MCC-3), 6% (MCC-6), and 9% (MCC-9) protein were prepared and standardized by mixing water, MCC powder, milk permeate, and cream to produce a solution with 14.7% total solids (TS) and 3.3% fat. Thermophilic cultures were added at a rate of 0.4% to MCC solutions and incubated at 35 °C for 3 h to get a pH of 6.1. Subsequently, calcium chloride and rennet were added to set the curd in 20 min at 35 °C. The curd was then cut into cubes, drained for 20 h followed by brining in 23% sodium chloride solutions for 24 h. Compositional analysis of MCC solutions and cheese was carried out. The yield, color, textural, and rheological measurements of Feta-type cheese were evaluated. Feta-type cheese was also made from whole milk as a control. This experiment was repeated three times. The yield and adjusted yield of Feta-type cheese increased from 19.0 to 54.8 and 21.4 to 56.5, respectively, with increasing the protein content in MCC from 3% to 9%. However, increasing the protein content in MCC did not show significant differences in the hardness (9.2–9.7 kg) of Feta-type cheese. The color of Feta-type cheese was less white with increasing the protein content in MCC. While the yellowish and greenish colors were high in Feta-type cheese made from MCC with 3% and 6% protein, no visible differences were found in the overall cheese color. The rheological characteristics were improved in Feta-type cheese made from MCC with 6% protein. We conclude that MCC with different levels of protein can be utilized in the manufacture of Feta-type cheese.
format Online
Article
Text
id pubmed-8750234
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-87502342022-01-12 Compositional and Functional Characteristics of Feta-Type Cheese Made from Micellar Casein Concentrate Hammam, Ahmed R. A. Kapoor, Rohit Salunke, Prafulla Metzger, Lloyd E. Foods Article Micellar casein concentrate (MCC) is a high protein ingredient (obtained by microfiltration of skim milk) with an elevated level of casein as a percentage of total protein (TP) compared to skim milk. It can be used as an ingredient in cheese making. Feta-type cheese is a brined soft cheese with a salty taste and acid flavor. We theorize that Feta-type cheese can be produced from MCC instead of milk, which can improve the efficiency of manufacture and allow for the removal of whey proteins before manufacturing Feta-type cheese. The objectives of this study were to develop a process of producing Feta-type cheese from MCC and to determine the optimum protein content in MCC to make Feta-type cheese. MCC solutions with 3% (MCC-3), 6% (MCC-6), and 9% (MCC-9) protein were prepared and standardized by mixing water, MCC powder, milk permeate, and cream to produce a solution with 14.7% total solids (TS) and 3.3% fat. Thermophilic cultures were added at a rate of 0.4% to MCC solutions and incubated at 35 °C for 3 h to get a pH of 6.1. Subsequently, calcium chloride and rennet were added to set the curd in 20 min at 35 °C. The curd was then cut into cubes, drained for 20 h followed by brining in 23% sodium chloride solutions for 24 h. Compositional analysis of MCC solutions and cheese was carried out. The yield, color, textural, and rheological measurements of Feta-type cheese were evaluated. Feta-type cheese was also made from whole milk as a control. This experiment was repeated three times. The yield and adjusted yield of Feta-type cheese increased from 19.0 to 54.8 and 21.4 to 56.5, respectively, with increasing the protein content in MCC from 3% to 9%. However, increasing the protein content in MCC did not show significant differences in the hardness (9.2–9.7 kg) of Feta-type cheese. The color of Feta-type cheese was less white with increasing the protein content in MCC. While the yellowish and greenish colors were high in Feta-type cheese made from MCC with 3% and 6% protein, no visible differences were found in the overall cheese color. The rheological characteristics were improved in Feta-type cheese made from MCC with 6% protein. We conclude that MCC with different levels of protein can be utilized in the manufacture of Feta-type cheese. MDPI 2021-12-23 /pmc/articles/PMC8750234/ /pubmed/35010150 http://dx.doi.org/10.3390/foods11010024 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hammam, Ahmed R. A.
Kapoor, Rohit
Salunke, Prafulla
Metzger, Lloyd E.
Compositional and Functional Characteristics of Feta-Type Cheese Made from Micellar Casein Concentrate
title Compositional and Functional Characteristics of Feta-Type Cheese Made from Micellar Casein Concentrate
title_full Compositional and Functional Characteristics of Feta-Type Cheese Made from Micellar Casein Concentrate
title_fullStr Compositional and Functional Characteristics of Feta-Type Cheese Made from Micellar Casein Concentrate
title_full_unstemmed Compositional and Functional Characteristics of Feta-Type Cheese Made from Micellar Casein Concentrate
title_short Compositional and Functional Characteristics of Feta-Type Cheese Made from Micellar Casein Concentrate
title_sort compositional and functional characteristics of feta-type cheese made from micellar casein concentrate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8750234/
https://www.ncbi.nlm.nih.gov/pubmed/35010150
http://dx.doi.org/10.3390/foods11010024
work_keys_str_mv AT hammamahmedra compositionalandfunctionalcharacteristicsoffetatypecheesemadefrommicellarcaseinconcentrate
AT kapoorrohit compositionalandfunctionalcharacteristicsoffetatypecheesemadefrommicellarcaseinconcentrate
AT salunkeprafulla compositionalandfunctionalcharacteristicsoffetatypecheesemadefrommicellarcaseinconcentrate
AT metzgerlloyde compositionalandfunctionalcharacteristicsoffetatypecheesemadefrommicellarcaseinconcentrate