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Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm
Implant-associated Staphylococcus aureus infections are difficult to treat because of biofilm formation. Bacteria in a biofilm are often insensitive to antibiotics and host immunity. Monoclonal antibodies (mAbs) could provide an alternative approach to improve the diagnosis and potential treatment o...
Autores principales: | , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8751199/ https://www.ncbi.nlm.nih.gov/pubmed/34989676 http://dx.doi.org/10.7554/eLife.67301 |
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author | de Vor, Lisanne van Dijk, Bruce van Kessel, Kok Kavanaugh, Jeffrey S de Haas, Carla Aerts, Piet C Viveen, Marco C Boel, Edwin C Fluit, Ad C Kwiecinski, Jakub M Krijger, Gerard C Ramakers, Ruud M Beekman, Freek J Dadachova, Ekaterina Lam, Marnix GEH Vogely, H Charles van der Wal, Bart CH van Strijp, Jos AG Horswill, Alexander R Weinans, Harrie Rooijakkers, Suzan HM |
author_facet | de Vor, Lisanne van Dijk, Bruce van Kessel, Kok Kavanaugh, Jeffrey S de Haas, Carla Aerts, Piet C Viveen, Marco C Boel, Edwin C Fluit, Ad C Kwiecinski, Jakub M Krijger, Gerard C Ramakers, Ruud M Beekman, Freek J Dadachova, Ekaterina Lam, Marnix GEH Vogely, H Charles van der Wal, Bart CH van Strijp, Jos AG Horswill, Alexander R Weinans, Harrie Rooijakkers, Suzan HM |
author_sort | de Vor, Lisanne |
collection | PubMed |
description | Implant-associated Staphylococcus aureus infections are difficult to treat because of biofilm formation. Bacteria in a biofilm are often insensitive to antibiotics and host immunity. Monoclonal antibodies (mAbs) could provide an alternative approach to improve the diagnosis and potential treatment of biofilm-related infections. Here, we show that mAbs targeting common surface components of S. aureus can recognize clinically relevant biofilm types. The mAbs were also shown to bind a collection of clinical isolates derived from different biofilm-associated infections (endocarditis, prosthetic joint, catheter). We identify two groups of antibodies: one group that uniquely binds S. aureus in biofilm state and one that recognizes S. aureus in both biofilm and planktonic state. Furthermore, we show that a mAb recognizing wall teichoic acid (clone 4497) specifically localizes to a subcutaneously implanted pre-colonized catheter in mice. In conclusion, we demonstrate the capacity of several human mAbs to detect S. aureus biofilms in vitro and in vivo. |
format | Online Article Text |
id | pubmed-8751199 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-87511992022-01-12 Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm de Vor, Lisanne van Dijk, Bruce van Kessel, Kok Kavanaugh, Jeffrey S de Haas, Carla Aerts, Piet C Viveen, Marco C Boel, Edwin C Fluit, Ad C Kwiecinski, Jakub M Krijger, Gerard C Ramakers, Ruud M Beekman, Freek J Dadachova, Ekaterina Lam, Marnix GEH Vogely, H Charles van der Wal, Bart CH van Strijp, Jos AG Horswill, Alexander R Weinans, Harrie Rooijakkers, Suzan HM eLife Microbiology and Infectious Disease Implant-associated Staphylococcus aureus infections are difficult to treat because of biofilm formation. Bacteria in a biofilm are often insensitive to antibiotics and host immunity. Monoclonal antibodies (mAbs) could provide an alternative approach to improve the diagnosis and potential treatment of biofilm-related infections. Here, we show that mAbs targeting common surface components of S. aureus can recognize clinically relevant biofilm types. The mAbs were also shown to bind a collection of clinical isolates derived from different biofilm-associated infections (endocarditis, prosthetic joint, catheter). We identify two groups of antibodies: one group that uniquely binds S. aureus in biofilm state and one that recognizes S. aureus in both biofilm and planktonic state. Furthermore, we show that a mAb recognizing wall teichoic acid (clone 4497) specifically localizes to a subcutaneously implanted pre-colonized catheter in mice. In conclusion, we demonstrate the capacity of several human mAbs to detect S. aureus biofilms in vitro and in vivo. eLife Sciences Publications, Ltd 2022-01-06 /pmc/articles/PMC8751199/ /pubmed/34989676 http://dx.doi.org/10.7554/eLife.67301 Text en © 2022, de Vor et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Microbiology and Infectious Disease de Vor, Lisanne van Dijk, Bruce van Kessel, Kok Kavanaugh, Jeffrey S de Haas, Carla Aerts, Piet C Viveen, Marco C Boel, Edwin C Fluit, Ad C Kwiecinski, Jakub M Krijger, Gerard C Ramakers, Ruud M Beekman, Freek J Dadachova, Ekaterina Lam, Marnix GEH Vogely, H Charles van der Wal, Bart CH van Strijp, Jos AG Horswill, Alexander R Weinans, Harrie Rooijakkers, Suzan HM Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm |
title | Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm |
title_full | Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm |
title_fullStr | Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm |
title_full_unstemmed | Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm |
title_short | Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm |
title_sort | human monoclonal antibodies against staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm |
topic | Microbiology and Infectious Disease |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8751199/ https://www.ncbi.nlm.nih.gov/pubmed/34989676 http://dx.doi.org/10.7554/eLife.67301 |
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