Cargando…
NeuroTrace 500/525 identifies human induced pluripotent stem cell-derived brain pericyte-like cells
In the CNS, pericytes are important for maintaining the blood–brain barrier (BBB) and for controlling blood flow. Recently, several methods were suggested for the differentiation of human pluripotent stem cells (hPSCs) into brain mural cells, specifically pericytes or vascular smooth muscle cells (v...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8751259/ https://www.ncbi.nlm.nih.gov/pubmed/35012603 http://dx.doi.org/10.1186/s13041-021-00893-5 |
_version_ | 1784631646004183040 |
---|---|
author | Kim, Seo Young Choi, Jihye Roh, Junhee Kim, Chul Hoon |
author_facet | Kim, Seo Young Choi, Jihye Roh, Junhee Kim, Chul Hoon |
author_sort | Kim, Seo Young |
collection | PubMed |
description | In the CNS, pericytes are important for maintaining the blood–brain barrier (BBB) and for controlling blood flow. Recently, several methods were suggested for the differentiation of human pluripotent stem cells (hPSCs) into brain mural cells, specifically pericytes or vascular smooth muscle cells (vSMCs). Unfortunately, identifying the pericytes from among such hPSC-derived mural cells has been challenging. This is due both to the lack of pericyte-specific markers and to the loss of defining anatomical information inherent to culture conditions. We therefore asked whether NeuroTrace 500/525, a newly developed dye that shows cell-specific uptake into pericytes in the mouse brain, can help identify human induced pluripotent stem cell (hiPSC)-derived brain pericyte-like cells. First, we found that NeuroTrace 500/525 specifically stains primary cultured human brain pericytes, confirming its specificity in vitro. Second, we found that NeuroTrace 500/525 specifically labels hiPSC-derived pericyte-like cells, but not endothelial cells or vSMCs derived from the same hiPSCs. Last, we found that neuroectoderm-derived vSMCs, which have pericyte-like features, also take up NeuroTrace 500/525. These data indicate NeuroTrace 500/525 is useful for identifying pericyte-like cells among hiPSC-derived brain mural cells. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13041-021-00893-5. |
format | Online Article Text |
id | pubmed-8751259 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-87512592022-01-11 NeuroTrace 500/525 identifies human induced pluripotent stem cell-derived brain pericyte-like cells Kim, Seo Young Choi, Jihye Roh, Junhee Kim, Chul Hoon Mol Brain Micro Report In the CNS, pericytes are important for maintaining the blood–brain barrier (BBB) and for controlling blood flow. Recently, several methods were suggested for the differentiation of human pluripotent stem cells (hPSCs) into brain mural cells, specifically pericytes or vascular smooth muscle cells (vSMCs). Unfortunately, identifying the pericytes from among such hPSC-derived mural cells has been challenging. This is due both to the lack of pericyte-specific markers and to the loss of defining anatomical information inherent to culture conditions. We therefore asked whether NeuroTrace 500/525, a newly developed dye that shows cell-specific uptake into pericytes in the mouse brain, can help identify human induced pluripotent stem cell (hiPSC)-derived brain pericyte-like cells. First, we found that NeuroTrace 500/525 specifically stains primary cultured human brain pericytes, confirming its specificity in vitro. Second, we found that NeuroTrace 500/525 specifically labels hiPSC-derived pericyte-like cells, but not endothelial cells or vSMCs derived from the same hiPSCs. Last, we found that neuroectoderm-derived vSMCs, which have pericyte-like features, also take up NeuroTrace 500/525. These data indicate NeuroTrace 500/525 is useful for identifying pericyte-like cells among hiPSC-derived brain mural cells. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13041-021-00893-5. BioMed Central 2022-01-10 /pmc/articles/PMC8751259/ /pubmed/35012603 http://dx.doi.org/10.1186/s13041-021-00893-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Micro Report Kim, Seo Young Choi, Jihye Roh, Junhee Kim, Chul Hoon NeuroTrace 500/525 identifies human induced pluripotent stem cell-derived brain pericyte-like cells |
title | NeuroTrace 500/525 identifies human induced pluripotent stem cell-derived brain pericyte-like cells |
title_full | NeuroTrace 500/525 identifies human induced pluripotent stem cell-derived brain pericyte-like cells |
title_fullStr | NeuroTrace 500/525 identifies human induced pluripotent stem cell-derived brain pericyte-like cells |
title_full_unstemmed | NeuroTrace 500/525 identifies human induced pluripotent stem cell-derived brain pericyte-like cells |
title_short | NeuroTrace 500/525 identifies human induced pluripotent stem cell-derived brain pericyte-like cells |
title_sort | neurotrace 500/525 identifies human induced pluripotent stem cell-derived brain pericyte-like cells |
topic | Micro Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8751259/ https://www.ncbi.nlm.nih.gov/pubmed/35012603 http://dx.doi.org/10.1186/s13041-021-00893-5 |
work_keys_str_mv | AT kimseoyoung neurotrace500525identifieshumaninducedpluripotentstemcellderivedbrainpericytelikecells AT choijihye neurotrace500525identifieshumaninducedpluripotentstemcellderivedbrainpericytelikecells AT rohjunhee neurotrace500525identifieshumaninducedpluripotentstemcellderivedbrainpericytelikecells AT kimchulhoon neurotrace500525identifieshumaninducedpluripotentstemcellderivedbrainpericytelikecells |