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CXCL17 Is Dispensable during Hypervirulent Mycobacterium tuberculosis HN878 Infection in Mice

CXCL17 is a novel mucosal chemokine that mediates myeloid cell recruitment and bactericidal activity and highly expressed in the respiratory tract. However, its role in tuberculosis (TB) immunopathogenesis or protection remains unknown. In this study, we evaluated the function of CXCL17 in a mouse m...

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Autores principales: Choreño-Parra, José Alberto, Dunlap, Micah D., Swanson, Rosemary, Jiménez-Álvarez, Luis A., Muñoz-Torrico, Marcela, Guzmán-Beltrán, Silvia, Zúñiga, Joaquín, Khader, Shabaana A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8751481/
https://www.ncbi.nlm.nih.gov/pubmed/34561226
http://dx.doi.org/10.4049/immunohorizons.2100048
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author Choreño-Parra, José Alberto
Dunlap, Micah D.
Swanson, Rosemary
Jiménez-Álvarez, Luis A.
Muñoz-Torrico, Marcela
Guzmán-Beltrán, Silvia
Zúñiga, Joaquín
Khader, Shabaana A.
author_facet Choreño-Parra, José Alberto
Dunlap, Micah D.
Swanson, Rosemary
Jiménez-Álvarez, Luis A.
Muñoz-Torrico, Marcela
Guzmán-Beltrán, Silvia
Zúñiga, Joaquín
Khader, Shabaana A.
author_sort Choreño-Parra, José Alberto
collection PubMed
description CXCL17 is a novel mucosal chemokine that mediates myeloid cell recruitment and bactericidal activity and highly expressed in the respiratory tract. However, its role in tuberculosis (TB) immunopathogenesis or protection remains unknown. In this study, we evaluated the function of CXCL17 in a mouse model of aerosol infection with the clinical W-Beijing lineage Mycobacterium tuberculosis hypervirulent HN878 strain. Our results show that CXCL17 production increases in the lung of M. tuberculosis–infected mice during acute and chronic stages of infection. Moreover, in vitro M. tuberculosis infection of epithelial cells and myeloid cells induces production of CXCL17. In humans, lower serum CXCL17 levels are observed among active pulmonary TB patients when compared with subjects with latent TB infection and healthy controls, suggesting a protective role. However, mice treated with rCXCL17 show similar lung bacterial burden and inflammation compared with control animals, despite an increased lung myeloid cell accumulation. Finally, CXCL17(−/−) mice are not more susceptible to TB than wild-type animals. These findings suggest that CXCL17 is induced in both murine epithelial and myeloid cells upon M. tuberculosis infection and increased expression during human latent TB infection. However, CXCL17 may have a dispensable role during pulmonary TB.
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spelling pubmed-87514812022-03-24 CXCL17 Is Dispensable during Hypervirulent Mycobacterium tuberculosis HN878 Infection in Mice Choreño-Parra, José Alberto Dunlap, Micah D. Swanson, Rosemary Jiménez-Álvarez, Luis A. Muñoz-Torrico, Marcela Guzmán-Beltrán, Silvia Zúñiga, Joaquín Khader, Shabaana A. Immunohorizons Article CXCL17 is a novel mucosal chemokine that mediates myeloid cell recruitment and bactericidal activity and highly expressed in the respiratory tract. However, its role in tuberculosis (TB) immunopathogenesis or protection remains unknown. In this study, we evaluated the function of CXCL17 in a mouse model of aerosol infection with the clinical W-Beijing lineage Mycobacterium tuberculosis hypervirulent HN878 strain. Our results show that CXCL17 production increases in the lung of M. tuberculosis–infected mice during acute and chronic stages of infection. Moreover, in vitro M. tuberculosis infection of epithelial cells and myeloid cells induces production of CXCL17. In humans, lower serum CXCL17 levels are observed among active pulmonary TB patients when compared with subjects with latent TB infection and healthy controls, suggesting a protective role. However, mice treated with rCXCL17 show similar lung bacterial burden and inflammation compared with control animals, despite an increased lung myeloid cell accumulation. Finally, CXCL17(−/−) mice are not more susceptible to TB than wild-type animals. These findings suggest that CXCL17 is induced in both murine epithelial and myeloid cells upon M. tuberculosis infection and increased expression during human latent TB infection. However, CXCL17 may have a dispensable role during pulmonary TB. 2021-09-24 /pmc/articles/PMC8751481/ /pubmed/34561226 http://dx.doi.org/10.4049/immunohorizons.2100048 Text en https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the CC BY 4.0 Unported license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Choreño-Parra, José Alberto
Dunlap, Micah D.
Swanson, Rosemary
Jiménez-Álvarez, Luis A.
Muñoz-Torrico, Marcela
Guzmán-Beltrán, Silvia
Zúñiga, Joaquín
Khader, Shabaana A.
CXCL17 Is Dispensable during Hypervirulent Mycobacterium tuberculosis HN878 Infection in Mice
title CXCL17 Is Dispensable during Hypervirulent Mycobacterium tuberculosis HN878 Infection in Mice
title_full CXCL17 Is Dispensable during Hypervirulent Mycobacterium tuberculosis HN878 Infection in Mice
title_fullStr CXCL17 Is Dispensable during Hypervirulent Mycobacterium tuberculosis HN878 Infection in Mice
title_full_unstemmed CXCL17 Is Dispensable during Hypervirulent Mycobacterium tuberculosis HN878 Infection in Mice
title_short CXCL17 Is Dispensable during Hypervirulent Mycobacterium tuberculosis HN878 Infection in Mice
title_sort cxcl17 is dispensable during hypervirulent mycobacterium tuberculosis hn878 infection in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8751481/
https://www.ncbi.nlm.nih.gov/pubmed/34561226
http://dx.doi.org/10.4049/immunohorizons.2100048
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