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Low Doses of Sucralose Alter Fecal Microbiota in High-Fat Diet-Induced Obese Rats

Artificial sweeteners (AS) have been widely used as sugar substitutes to reduce calorie intake. However, it was reported that high doses of AS induced glucose intolerance via modulating gut microbiota. The objective of this study was to investigate the effects of lower doses of sucralose on fecal mi...

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Autores principales: Zhang, Minchun, Chen, Jie, Yang, Minglan, Qian, Cheng, Liu, Yu, Qi, Yicheng, Feng, Rilu, Yang, Mei, Liu, Wei, Ma, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8751733/
https://www.ncbi.nlm.nih.gov/pubmed/35028307
http://dx.doi.org/10.3389/fnut.2021.787055
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author Zhang, Minchun
Chen, Jie
Yang, Minglan
Qian, Cheng
Liu, Yu
Qi, Yicheng
Feng, Rilu
Yang, Mei
Liu, Wei
Ma, Jing
author_facet Zhang, Minchun
Chen, Jie
Yang, Minglan
Qian, Cheng
Liu, Yu
Qi, Yicheng
Feng, Rilu
Yang, Mei
Liu, Wei
Ma, Jing
author_sort Zhang, Minchun
collection PubMed
description Artificial sweeteners (AS) have been widely used as sugar substitutes to reduce calorie intake. However, it was reported that high doses of AS induced glucose intolerance via modulating gut microbiota. The objective of this study was to investigate the effects of lower doses of sucralose on fecal microbiota in obesity. Eight weeks after high-fat diet (HFD), the male Sprague Dawley rats were randomly divided into four groups (6 in each group) and administrated by a daily gavage of 2 ml normal saline (CON), 0.54 mM sucralose (N054), 0.78 mM sucralose (N078), and 324 mM sucrose (S324), respectively. After 4 weeks, fecal samples were obtained and analyzed by 16S ribosomal RNA gene sequencing. The richness and diversity of fecal microbiota were not changed by sucralose or sucrose. Both 0.54 mM (0.43 mg) and 0.78 mM (0.62 mg) sucralose tended to reduce the beneficial bacteria, Lactobacillaceae and Akkermansiaceae. The relative abundance of family Acidaminoccaceae and its genus Phascolarctobacteriam were increased after 0.54 mM sucralose. In functional prediction, 0.54 mM sucralose increased profiles of carbohydrate metabolism, whereas 0.78 mM sucralose enhanced those of amino acid metabolism. The lower doses of sucralose might alter the compositions of fecal microbiota. The effects of sucralose in different dosages should be considered in the future study.
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spelling pubmed-87517332022-01-12 Low Doses of Sucralose Alter Fecal Microbiota in High-Fat Diet-Induced Obese Rats Zhang, Minchun Chen, Jie Yang, Minglan Qian, Cheng Liu, Yu Qi, Yicheng Feng, Rilu Yang, Mei Liu, Wei Ma, Jing Front Nutr Nutrition Artificial sweeteners (AS) have been widely used as sugar substitutes to reduce calorie intake. However, it was reported that high doses of AS induced glucose intolerance via modulating gut microbiota. The objective of this study was to investigate the effects of lower doses of sucralose on fecal microbiota in obesity. Eight weeks after high-fat diet (HFD), the male Sprague Dawley rats were randomly divided into four groups (6 in each group) and administrated by a daily gavage of 2 ml normal saline (CON), 0.54 mM sucralose (N054), 0.78 mM sucralose (N078), and 324 mM sucrose (S324), respectively. After 4 weeks, fecal samples were obtained and analyzed by 16S ribosomal RNA gene sequencing. The richness and diversity of fecal microbiota were not changed by sucralose or sucrose. Both 0.54 mM (0.43 mg) and 0.78 mM (0.62 mg) sucralose tended to reduce the beneficial bacteria, Lactobacillaceae and Akkermansiaceae. The relative abundance of family Acidaminoccaceae and its genus Phascolarctobacteriam were increased after 0.54 mM sucralose. In functional prediction, 0.54 mM sucralose increased profiles of carbohydrate metabolism, whereas 0.78 mM sucralose enhanced those of amino acid metabolism. The lower doses of sucralose might alter the compositions of fecal microbiota. The effects of sucralose in different dosages should be considered in the future study. Frontiers Media S.A. 2021-12-28 /pmc/articles/PMC8751733/ /pubmed/35028307 http://dx.doi.org/10.3389/fnut.2021.787055 Text en Copyright © 2021 Zhang, Chen, Yang, Qian, Liu, Qi, Feng, Yang, Liu and Ma. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Zhang, Minchun
Chen, Jie
Yang, Minglan
Qian, Cheng
Liu, Yu
Qi, Yicheng
Feng, Rilu
Yang, Mei
Liu, Wei
Ma, Jing
Low Doses of Sucralose Alter Fecal Microbiota in High-Fat Diet-Induced Obese Rats
title Low Doses of Sucralose Alter Fecal Microbiota in High-Fat Diet-Induced Obese Rats
title_full Low Doses of Sucralose Alter Fecal Microbiota in High-Fat Diet-Induced Obese Rats
title_fullStr Low Doses of Sucralose Alter Fecal Microbiota in High-Fat Diet-Induced Obese Rats
title_full_unstemmed Low Doses of Sucralose Alter Fecal Microbiota in High-Fat Diet-Induced Obese Rats
title_short Low Doses of Sucralose Alter Fecal Microbiota in High-Fat Diet-Induced Obese Rats
title_sort low doses of sucralose alter fecal microbiota in high-fat diet-induced obese rats
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8751733/
https://www.ncbi.nlm.nih.gov/pubmed/35028307
http://dx.doi.org/10.3389/fnut.2021.787055
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