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Irisin protects female mice with LPS-induced endometritis through the AMPK/NF-κB pathway
OBJECTIVE(S): This research was designed to determine the role of irisin in lipopolysaccharide (LPS)-induced endometritis in female mice. MATERIALS AND METHODS: Animals were randomly assigned into sham, sham + irisin, LPS, LPS + irisin (0.1, 1, 10 μg/kg), and LPS + irisin + compound C groups. Histol...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mashhad University of Medical Sciences
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8751749/ https://www.ncbi.nlm.nih.gov/pubmed/35083012 http://dx.doi.org/10.22038/ijbms.2021.56781.12678 |
Sumario: | OBJECTIVE(S): This research was designed to determine the role of irisin in lipopolysaccharide (LPS)-induced endometritis in female mice. MATERIALS AND METHODS: Animals were randomly assigned into sham, sham + irisin, LPS, LPS + irisin (0.1, 1, 10 μg/kg), and LPS + irisin + compound C groups. Histological features and expression of AMPK, NF-κB, inflammatory mediators, and oxidative stress markers were compared among different groups. RESULTS: The results showed that LPS resulted in obvious uterus damage, meanwhile, the inflammatory mediators (COX-2, iNOS, IL-1β, IL-6, and TNF-α), as well as NF-κB in the uterine tissue, were significantly increased and the level of adenosine monophosphate-activated protein kinase (AMPK) was reduced. Nevertheless, pretreatment with irisin reversed the phenomena caused by LPS. Interestingly, compound C (AMPK inhibitor) abolished irisin’s effects on the uterus, which suggested that irisin’s beneficial function was achieved through regulating the AMPK-NF-κB pathway. Moreover, LPS-induced alterations of oxidative factors (MnSOD, GSH, and MDA) were reversed significantly by pretreatment with irisin. This data indicated irisin’s beneficial function was also related to antioxidation besides anti-inflammation. CONCLUSION: Our study implies that irisin is a potential therapeutic agent for endometritis. |
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