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Directed evolution of the rRNA methylating enzyme Cfr reveals molecular basis of antibiotic resistance

Alteration of antibiotic binding sites through modification of ribosomal RNA (rRNA) is a common form of resistance to ribosome-targeting antibiotics. The rRNA-modifying enzyme Cfr methylates an adenosine nucleotide within the peptidyl transferase center, resulting in the C-8 methylation of A2503 (m(...

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Detalles Bibliográficos
Autores principales:	Tsai, Kaitlyn, Stojković, Vanja, Noda-Garcia, Lianet, Young, Iris D, Myasnikov, Alexander G, Kleinman, Jordan, Palla, Ali, Floor, Stephen N, Frost, Adam, Fraser, James S, Tawfik, Dan S, Fujimori, Danica Galonić
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	eLife Sciences Publications, Ltd 2022
Materias:	Biochemistry and Chemical Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8752094/ https://www.ncbi.nlm.nih.gov/pubmed/35015630 http://dx.doi.org/10.7554/eLife.70017

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