Cargando…

Directed evolution of the rRNA methylating enzyme Cfr reveals molecular basis of antibiotic resistance

Alteration of antibiotic binding sites through modification of ribosomal RNA (rRNA) is a common form of resistance to ribosome-targeting antibiotics. The rRNA-modifying enzyme Cfr methylates an adenosine nucleotide within the peptidyl transferase center, resulting in the C-8 methylation of A2503 (m(...

Descripción completa

Detalles Bibliográficos
Autores principales:	Tsai, Kaitlyn, Stojković, Vanja, Noda-Garcia, Lianet, Young, Iris D, Myasnikov, Alexander G, Kleinman, Jordan, Palla, Ali, Floor, Stephen N, Frost, Adam, Fraser, James S, Tawfik, Dan S, Fujimori, Danica Galonić
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	eLife Sciences Publications, Ltd 2022
Materias:	Biochemistry and Chemical Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8752094/ https://www.ncbi.nlm.nih.gov/pubmed/35015630 http://dx.doi.org/10.7554/eLife.70017

Ejemplares similares

Antibiotic resistance evolved via inactivation of a ribosomal RNA methylating enzyme
por: Stojković, Vanja, et al.
Publicado: (2016)

Assessment of the nucleotide modifications in the high-resolution cryo-electron microscopy structure of the Escherichia coli 50S subunit
por: Stojković, Vanja, et al.
Publicado: (2020)

Biochemical basis for the regulation of biosynthesis of antiparasitics by bacterial hormones
por: Kapoor, Iti, et al.
Publicado: (2020)

Structural basis for an unprecedented enzymatic alkylation in cylindrocyclophane biosynthesis
por: Braffman, Nathaniel R, et al.
Publicado: (2022)

Molecular basis for multimerization in the activation of the epidermal growth factor receptor
por: Huang, Yongjian, et al.
Publicado: (2016)

Basis of specificity for a conserved and promiscuous chromatin remodeling protein
por: Donovan, Drake A, et al.
Publicado: (2021)

Structural basis of proton translocation and force generation in mitochondrial ATP synthase
por: Klusch, Niklas, et al.
Publicado: (2017)

Molecular basis for the adaptive evolution of environment-sensing by H-NS proteins
por: Zhao, Xiaochuan, et al.
Publicado: (2021)

Molecular basis of ligand-dependent Nurr1-RXRα activation
por: Yu, Xiaoyu, et al.
Publicado: (2023)

The structural basis of the multi-step allosteric activation of Aurora B kinase
por: Segura-Peña, Dario, et al.
Publicado: (2023)

Molecular basis for substrate specificity of the Phactr1/PP1 phosphatase holoenzyme
por: Fedoryshchak, Roman O, et al.
Publicado: (2020)

Structural basis for the activation of PLC-γ isozymes by phosphorylation and cancer-associated mutations
por: Hajicek, Nicole, et al.
Publicado: (2019)

New approach for membrane protein reconstitution into peptidiscs and basis for their adaptability to different proteins
por: Angiulli, Gabriella, et al.
Publicado: (2020)

Uncovering the basis of protein-protein interaction specificity with a combinatorially complete library
por: Lite, Thuy-Lan V, et al.
Publicado: (2020)

Molecular basis for activation of lecithin:cholesterol acyltransferase by a compound that increases HDL cholesterol
por: Manthei, Kelly A, et al.
Publicado: (2018)

Structural basis for AcrVA4 inhibition of specific CRISPR-Cas12a
por: Knott, Gavin J, et al.
Publicado: (2019)

Mechanistic basis of the inhibition of SLC11/NRAMP-mediated metal ion transport by bis-isothiourea substituted compounds
por: Manatschal, Cristina, et al.
Publicado: (2019)

Structural basis for the recognition of spliceosomal SmN/B/B’ proteins by the RBM5 OCRE domain in splicing regulation
por: Mourão, André, et al.
Publicado: (2016)

Molecular basis for catabolism of the abundant metabolite trans-4-hydroxy-L-proline by a microbial glycyl radical enzyme
por: Backman, Lindsey RF, et al.
Publicado: (2020)

Molecular basis of the PIP(2)-dependent regulation of Ca(V)2.2 channel and its modulation by Ca(V) β subunits
por: Park, Cheon-Gyu, et al.
Publicado: (2022)

Determinants of tRNA Recognition by the Radical SAM Enzyme RlmN
por: Fitzsimmons, Christina M., et al.
Publicado: (2016)

ORACLE reveals a bright future to fight bacteria
por: Coyote-Maestas, Willow, et al.
Publicado: (2021)

eIF2B conformation and assembly state regulate the integrated stress response
por: Schoof, Michael, et al.
Publicado: (2021)

A point mutation in the nucleotide exchange factor eIF2B constitutively activates the integrated stress response by allosteric modulation
por: Boone, Morgane, et al.
Publicado: (2022)

Cysteine Methylation Controls Radical Generation in the Cfr Radical AdoMet rRNA Methyltransferase
por: Challand, Martin R., et al.
Publicado: (2013)

Structural basis of Cfr-mediated antimicrobial resistance and mechanisms for its evasion
por: Aleksandrova, Elena V., et al.
Publicado: (2023)

Chromatin Sensing by the Auxiliary Domains of KDM5C Regulates Its Demethylase Activity and Is Disrupted by X-linked Intellectual Disability Mutations
por: Ugur, Fatima S., et al.
Publicado: (2023)

Chemical tools targeting readers of lysine methylation
por: Ortiz, Gloria, et al.
Publicado: (2023)

Mitochondrial pyruvate carrier is required for optimal brown fat thermogenesis
por: Panic, Vanja, et al.
Publicado: (2020)

Rhodoquinone biosynthesis in C. elegans requires precursors generated by the kynurenine pathway
por: Del Borrello, Samantha, et al.
Publicado: (2019)

Molecular basis of outer kinetochore assembly on CENP-T
por: Huis in 't Veld, Pim J, et al.
Publicado: (2016)

Identification of host proteins differentially associated with HIV-1 RNA splice variants
por: Knoener, Rachel, et al.
Publicado: (2021)

Dissecting the phase separation and oligomerization activities of the carboxysome positioning protein McdB
por: Basalla, Joseph L, et al.
Publicado: (2023)

Schnyder corneal dystrophy-associated UBIAD1 inhibits ER-associated degradation of HMG CoA reductase in mice
por: Jo, Youngah, et al.
Publicado: (2019)

Evolution of substrate specificity in a retained enzyme driven by gene loss
por: Juárez-Vázquez, Ana Lilia, et al.
Publicado: (2017)

Structural basis of diverse membrane target recognitions by ankyrins
por: Wang, Chao, et al.
Publicado: (2014)

Structural basis for the disaggregase activity and regulation of Hsp104
por: Heuck, Alexander, et al.
Publicado: (2016)

Dynamics of allosteric regulation of the phospholipase C-γ isozymes upon recruitment to membranes
por: Siraliev-Perez, Edhriz, et al.
Publicado: (2022)

Integrative modeling reveals the molecular architecture of the intraflagellar transport A (IFT-A) complex
por: McCafferty, Caitlyn L, et al.
Publicado: (2022)

A widely distributed metalloenzyme class enables gut microbial metabolism of host- and diet-derived catechols
por: Maini Rekdal, Vayu, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_9fnbc1kb4e0tkq3qv5t9189lr9