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High fat diet-induced obesity prolongs critical stages of the spermatogenic cycle in a Ldlr(−/−).Leiden mouse model

Obesity can disturb spermatogenesis and subsequently affect male fertility and reproduction. In our study, we aim to elucidate at which cellular level of adult spermatogenesis the detrimental effects of obesity manifest. We induced high fat diet (HFD) obesity in low-density lipoprotein receptor knoc...

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Autores principales: Komninos, D., Ramos, L., van der Heijden, G. W., Morrison, M. C., Kleemann, R., van Herwaarden, A. E., Kiliaan, A. J., Arnoldussen, I. A. C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8752771/
https://www.ncbi.nlm.nih.gov/pubmed/35017550
http://dx.doi.org/10.1038/s41598-021-04069-y
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author Komninos, D.
Ramos, L.
van der Heijden, G. W.
Morrison, M. C.
Kleemann, R.
van Herwaarden, A. E.
Kiliaan, A. J.
Arnoldussen, I. A. C.
author_facet Komninos, D.
Ramos, L.
van der Heijden, G. W.
Morrison, M. C.
Kleemann, R.
van Herwaarden, A. E.
Kiliaan, A. J.
Arnoldussen, I. A. C.
author_sort Komninos, D.
collection PubMed
description Obesity can disturb spermatogenesis and subsequently affect male fertility and reproduction. In our study, we aim to elucidate at which cellular level of adult spermatogenesis the detrimental effects of obesity manifest. We induced high fat diet (HFD) obesity in low-density lipoprotein receptor knock-out Leiden (Ldlr(−/−).Leiden) mice, and studied the morphological structure of the testes and histologically examined the proportion of Sertoli cells, spermatocytes and spermatids in the seminiferous tubules. We examined sperm DNA damage and chromatin condensation and measured plasma levels of leptin, testosterone, cholesterol and triglycerides. HFD-induced obesity caused high plasma leptin and abnormal testosterone levels and induced an aberrant intra-tubular organisation (ITO) which is associated with an altered spermatids/spermatocytes ratio (2:1 instead of 3:1). Mice fed a HFD had a higher level of tubules in stages VII + VIII in the spermatogenic cycle. The stages VII + VII indicate crucial processes in spermatogenic development like initiation of meiosis, initiation of spermatid elongation, and release of fully matured spermatids. In conclusion, HFD-induced obese Ldlr(−/−).Leiden mice develop an aberrant ITO and alterations in the spermatogenic cycle in crucial stages (stages VII and VII). Thereby, our findings stress the importance of lifestyle guidelines in infertility treatments.
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spelling pubmed-87527712022-01-13 High fat diet-induced obesity prolongs critical stages of the spermatogenic cycle in a Ldlr(−/−).Leiden mouse model Komninos, D. Ramos, L. van der Heijden, G. W. Morrison, M. C. Kleemann, R. van Herwaarden, A. E. Kiliaan, A. J. Arnoldussen, I. A. C. Sci Rep Article Obesity can disturb spermatogenesis and subsequently affect male fertility and reproduction. In our study, we aim to elucidate at which cellular level of adult spermatogenesis the detrimental effects of obesity manifest. We induced high fat diet (HFD) obesity in low-density lipoprotein receptor knock-out Leiden (Ldlr(−/−).Leiden) mice, and studied the morphological structure of the testes and histologically examined the proportion of Sertoli cells, spermatocytes and spermatids in the seminiferous tubules. We examined sperm DNA damage and chromatin condensation and measured plasma levels of leptin, testosterone, cholesterol and triglycerides. HFD-induced obesity caused high plasma leptin and abnormal testosterone levels and induced an aberrant intra-tubular organisation (ITO) which is associated with an altered spermatids/spermatocytes ratio (2:1 instead of 3:1). Mice fed a HFD had a higher level of tubules in stages VII + VIII in the spermatogenic cycle. The stages VII + VII indicate crucial processes in spermatogenic development like initiation of meiosis, initiation of spermatid elongation, and release of fully matured spermatids. In conclusion, HFD-induced obese Ldlr(−/−).Leiden mice develop an aberrant ITO and alterations in the spermatogenic cycle in crucial stages (stages VII and VII). Thereby, our findings stress the importance of lifestyle guidelines in infertility treatments. Nature Publishing Group UK 2022-01-11 /pmc/articles/PMC8752771/ /pubmed/35017550 http://dx.doi.org/10.1038/s41598-021-04069-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Komninos, D.
Ramos, L.
van der Heijden, G. W.
Morrison, M. C.
Kleemann, R.
van Herwaarden, A. E.
Kiliaan, A. J.
Arnoldussen, I. A. C.
High fat diet-induced obesity prolongs critical stages of the spermatogenic cycle in a Ldlr(−/−).Leiden mouse model
title High fat diet-induced obesity prolongs critical stages of the spermatogenic cycle in a Ldlr(−/−).Leiden mouse model
title_full High fat diet-induced obesity prolongs critical stages of the spermatogenic cycle in a Ldlr(−/−).Leiden mouse model
title_fullStr High fat diet-induced obesity prolongs critical stages of the spermatogenic cycle in a Ldlr(−/−).Leiden mouse model
title_full_unstemmed High fat diet-induced obesity prolongs critical stages of the spermatogenic cycle in a Ldlr(−/−).Leiden mouse model
title_short High fat diet-induced obesity prolongs critical stages of the spermatogenic cycle in a Ldlr(−/−).Leiden mouse model
title_sort high fat diet-induced obesity prolongs critical stages of the spermatogenic cycle in a ldlr(−/−).leiden mouse model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8752771/
https://www.ncbi.nlm.nih.gov/pubmed/35017550
http://dx.doi.org/10.1038/s41598-021-04069-y
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