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Circular RNA circSmoc1-2 regulates vascular calcification by acting as a miR-874-3p sponge in vascular smooth muscle cells
Vascular calcification (VC), or calcium deposition inside the blood vessels, is common in patients with atherosclerosis, cardiovascular disease, and chronic kidney disease. Although several treatments are available to reduce calcification, the incidence of VC continues to rise. Recently, there have...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society of Gene & Cell Therapy
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8752879/ https://www.ncbi.nlm.nih.gov/pubmed/35036071 http://dx.doi.org/10.1016/j.omtn.2021.12.031 |
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author | Ryu, Juhee Choe, Nakwon Kwon, Duk-Hwa Shin, Sera Lim, Yeong-Hwan Yoon, Gwangho Kim, Ji Hye Kim, Hyung Seok Lee, In-Kyu Ahn, Youngkeun Park, Woo Jin Kook, Hyun Kim, Young-Kook |
author_facet | Ryu, Juhee Choe, Nakwon Kwon, Duk-Hwa Shin, Sera Lim, Yeong-Hwan Yoon, Gwangho Kim, Ji Hye Kim, Hyung Seok Lee, In-Kyu Ahn, Youngkeun Park, Woo Jin Kook, Hyun Kim, Young-Kook |
author_sort | Ryu, Juhee |
collection | PubMed |
description | Vascular calcification (VC), or calcium deposition inside the blood vessels, is common in patients with atherosclerosis, cardiovascular disease, and chronic kidney disease. Although several treatments are available to reduce calcification, the incidence of VC continues to rise. Recently, there have been several reports describing the regulation of circular RNAs (circRNAs) in various diseases. However, the role of circRNAs in VC has not yet been fully explored. Here, we investigated the function of circSmoc1-2, one of the circRNAs generated from the Smoc1 gene, which is downregulated in response to VC. CircSmoc1-2 is localized primarily to the cytoplasm and is resistant to exonuclease digestion. Inhibition of circSmoc1-2 worsens VC, while overexpression of circSmoc1-2 reduces VC, suggesting that circSmoc1-2 can prevent calcification. We went on to investigate the mechanism of circSmoc1-2 as a microRNA sponge and noted that miR-874-3p, the predicted target of circSmoc1-2, promotes VC, while overexpression of circSmoc1-2 reduces VC by suppressing miR-874-3p. Additionally, we identified the potential mRNA target of miR-874-3p as Adam19. In conclusion, we revealed that the circSmoc1-2/miR-874-3p/Adam19 axis regulates VC, suggesting that circSmoc1-2 may be a novel therapeutic target in the treatment of VC. |
format | Online Article Text |
id | pubmed-8752879 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Society of Gene & Cell Therapy |
record_format | MEDLINE/PubMed |
spelling | pubmed-87528792022-01-14 Circular RNA circSmoc1-2 regulates vascular calcification by acting as a miR-874-3p sponge in vascular smooth muscle cells Ryu, Juhee Choe, Nakwon Kwon, Duk-Hwa Shin, Sera Lim, Yeong-Hwan Yoon, Gwangho Kim, Ji Hye Kim, Hyung Seok Lee, In-Kyu Ahn, Youngkeun Park, Woo Jin Kook, Hyun Kim, Young-Kook Mol Ther Nucleic Acids Original Article Vascular calcification (VC), or calcium deposition inside the blood vessels, is common in patients with atherosclerosis, cardiovascular disease, and chronic kidney disease. Although several treatments are available to reduce calcification, the incidence of VC continues to rise. Recently, there have been several reports describing the regulation of circular RNAs (circRNAs) in various diseases. However, the role of circRNAs in VC has not yet been fully explored. Here, we investigated the function of circSmoc1-2, one of the circRNAs generated from the Smoc1 gene, which is downregulated in response to VC. CircSmoc1-2 is localized primarily to the cytoplasm and is resistant to exonuclease digestion. Inhibition of circSmoc1-2 worsens VC, while overexpression of circSmoc1-2 reduces VC, suggesting that circSmoc1-2 can prevent calcification. We went on to investigate the mechanism of circSmoc1-2 as a microRNA sponge and noted that miR-874-3p, the predicted target of circSmoc1-2, promotes VC, while overexpression of circSmoc1-2 reduces VC by suppressing miR-874-3p. Additionally, we identified the potential mRNA target of miR-874-3p as Adam19. In conclusion, we revealed that the circSmoc1-2/miR-874-3p/Adam19 axis regulates VC, suggesting that circSmoc1-2 may be a novel therapeutic target in the treatment of VC. American Society of Gene & Cell Therapy 2021-12-22 /pmc/articles/PMC8752879/ /pubmed/35036071 http://dx.doi.org/10.1016/j.omtn.2021.12.031 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Ryu, Juhee Choe, Nakwon Kwon, Duk-Hwa Shin, Sera Lim, Yeong-Hwan Yoon, Gwangho Kim, Ji Hye Kim, Hyung Seok Lee, In-Kyu Ahn, Youngkeun Park, Woo Jin Kook, Hyun Kim, Young-Kook Circular RNA circSmoc1-2 regulates vascular calcification by acting as a miR-874-3p sponge in vascular smooth muscle cells |
title | Circular RNA circSmoc1-2 regulates vascular calcification by acting as a miR-874-3p sponge in vascular smooth muscle cells |
title_full | Circular RNA circSmoc1-2 regulates vascular calcification by acting as a miR-874-3p sponge in vascular smooth muscle cells |
title_fullStr | Circular RNA circSmoc1-2 regulates vascular calcification by acting as a miR-874-3p sponge in vascular smooth muscle cells |
title_full_unstemmed | Circular RNA circSmoc1-2 regulates vascular calcification by acting as a miR-874-3p sponge in vascular smooth muscle cells |
title_short | Circular RNA circSmoc1-2 regulates vascular calcification by acting as a miR-874-3p sponge in vascular smooth muscle cells |
title_sort | circular rna circsmoc1-2 regulates vascular calcification by acting as a mir-874-3p sponge in vascular smooth muscle cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8752879/ https://www.ncbi.nlm.nih.gov/pubmed/35036071 http://dx.doi.org/10.1016/j.omtn.2021.12.031 |
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