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Seroepidemiological survey on pigs and cattle for novel K88 (F4)-like colonisation factor detected in human enterotoxigenic Escherichia coli

Enterotoxigenic Escherichia coli (ETEC) strains that express various fimbrial or nonfimbrial colonisation factors (CFs) and enterotoxins are critical causes of diarrhoeal diseases. Human ETEC serotype O169:H41 (O169) has been a representative of epidemic ETEC worldwide; the organism shows massive ad...

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Autores principales: Tanimoto, Yoshihiko, Inoue, Miyoko, Komatsu, Kana, Odani, Atsuyuki, Wada, Takayuki, Kage-Nakadai, Eriko, Nishikawa, Yoshikazu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cambridge University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8753484/
http://dx.doi.org/10.1017/S0950268821002697
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author Tanimoto, Yoshihiko
Inoue, Miyoko
Komatsu, Kana
Odani, Atsuyuki
Wada, Takayuki
Kage-Nakadai, Eriko
Nishikawa, Yoshikazu
author_facet Tanimoto, Yoshihiko
Inoue, Miyoko
Komatsu, Kana
Odani, Atsuyuki
Wada, Takayuki
Kage-Nakadai, Eriko
Nishikawa, Yoshikazu
author_sort Tanimoto, Yoshihiko
collection PubMed
description Enterotoxigenic Escherichia coli (ETEC) strains that express various fimbrial or nonfimbrial colonisation factors (CFs) and enterotoxins are critical causes of diarrhoeal diseases. Human ETEC serotype O169:H41 (O169) has been a representative of epidemic ETEC worldwide; the organism shows massive adherence to HEp-2 cells similar to enteroaggregative E. coli. Previously, we determined the complete sequence of the unstable virulence plasmid, pEntYN10. The plasmid included a unique set of genes encoding a novel CF resembling K88 (F4) of porcine ETEC, in addition to CS6, a well-known representative CF of human ETEC, and another novel CF similar to CS8 (CFA/III) of human ETEC. In the present study, we focused on K88-like CF (hereafter, K88(O169)) that may allow the organisms to infect domestic livestock like original K88-harbouring strains that can cause diarrhoea in piglets. Samples were tested for antibodies against recombinant proteins of possible paralogous adhesins, FaeG1 and FaeG2, from K88(O169) and the FaeG of typical K88 (F4). The seroepidemiological study using recombinant antigens (two paralogs FaeG1 and FaeG2 from K88(O169)) showed reactivity of porcine (18.0%) and bovine (17.1%) sera to K88(O169) FaeG1 and/or FaeG2 antigens on indirect ELISA tests. These results suggest that E. coli with K88(O169) adhesin can infect various hosts, including pigs and cattle. This is the first report of domestic livestock having antibodies to K88(O169) of human ETEC. Although human ETEC had been thought to be distinguished from those of domestic animals based on CFs, zoonotic strains may conceal themselves among human ETEC organisms. The concept of One Health should be adopted to intervene in ETEC infections among animals and humans.
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spelling pubmed-87534842022-01-27 Seroepidemiological survey on pigs and cattle for novel K88 (F4)-like colonisation factor detected in human enterotoxigenic Escherichia coli Tanimoto, Yoshihiko Inoue, Miyoko Komatsu, Kana Odani, Atsuyuki Wada, Takayuki Kage-Nakadai, Eriko Nishikawa, Yoshikazu Epidemiol Infect Original Paper Enterotoxigenic Escherichia coli (ETEC) strains that express various fimbrial or nonfimbrial colonisation factors (CFs) and enterotoxins are critical causes of diarrhoeal diseases. Human ETEC serotype O169:H41 (O169) has been a representative of epidemic ETEC worldwide; the organism shows massive adherence to HEp-2 cells similar to enteroaggregative E. coli. Previously, we determined the complete sequence of the unstable virulence plasmid, pEntYN10. The plasmid included a unique set of genes encoding a novel CF resembling K88 (F4) of porcine ETEC, in addition to CS6, a well-known representative CF of human ETEC, and another novel CF similar to CS8 (CFA/III) of human ETEC. In the present study, we focused on K88-like CF (hereafter, K88(O169)) that may allow the organisms to infect domestic livestock like original K88-harbouring strains that can cause diarrhoea in piglets. Samples were tested for antibodies against recombinant proteins of possible paralogous adhesins, FaeG1 and FaeG2, from K88(O169) and the FaeG of typical K88 (F4). The seroepidemiological study using recombinant antigens (two paralogs FaeG1 and FaeG2 from K88(O169)) showed reactivity of porcine (18.0%) and bovine (17.1%) sera to K88(O169) FaeG1 and/or FaeG2 antigens on indirect ELISA tests. These results suggest that E. coli with K88(O169) adhesin can infect various hosts, including pigs and cattle. This is the first report of domestic livestock having antibodies to K88(O169) of human ETEC. Although human ETEC had been thought to be distinguished from those of domestic animals based on CFs, zoonotic strains may conceal themselves among human ETEC organisms. The concept of One Health should be adopted to intervene in ETEC infections among animals and humans. Cambridge University Press 2021-12-13 /pmc/articles/PMC8753484/ http://dx.doi.org/10.1017/S0950268821002697 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution and reproduction, provided the original article is properly cited.
spellingShingle Original Paper
Tanimoto, Yoshihiko
Inoue, Miyoko
Komatsu, Kana
Odani, Atsuyuki
Wada, Takayuki
Kage-Nakadai, Eriko
Nishikawa, Yoshikazu
Seroepidemiological survey on pigs and cattle for novel K88 (F4)-like colonisation factor detected in human enterotoxigenic Escherichia coli
title Seroepidemiological survey on pigs and cattle for novel K88 (F4)-like colonisation factor detected in human enterotoxigenic Escherichia coli
title_full Seroepidemiological survey on pigs and cattle for novel K88 (F4)-like colonisation factor detected in human enterotoxigenic Escherichia coli
title_fullStr Seroepidemiological survey on pigs and cattle for novel K88 (F4)-like colonisation factor detected in human enterotoxigenic Escherichia coli
title_full_unstemmed Seroepidemiological survey on pigs and cattle for novel K88 (F4)-like colonisation factor detected in human enterotoxigenic Escherichia coli
title_short Seroepidemiological survey on pigs and cattle for novel K88 (F4)-like colonisation factor detected in human enterotoxigenic Escherichia coli
title_sort seroepidemiological survey on pigs and cattle for novel k88 (f4)-like colonisation factor detected in human enterotoxigenic escherichia coli
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8753484/
http://dx.doi.org/10.1017/S0950268821002697
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