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Customized Flagelliform Spidroins Form Spider Silk-like Fibers at pH 8.0 with Outstanding Tensile Strength
[Image: see text] Spider flagelliform silk shows the best extensibility among various types of silk, but its biomimetic preparation has not been much studied. Herein, five customized flagelliform spidroins (FlSps: S and NTD(Fl)-Sn-CTD(Fl), n = 1–4), in which the repetitive region (S) and N-/C- termi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8753598/ https://www.ncbi.nlm.nih.gov/pubmed/34908395 http://dx.doi.org/10.1021/acsbiomaterials.1c01354 |
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author | Li, Xue Qi, Xingmei Cai, Yu-ming Sun, Yuan Wen, Rui Zhang, Rui Johansson, Jan Meng, Qing Chen, Gefei |
author_facet | Li, Xue Qi, Xingmei Cai, Yu-ming Sun, Yuan Wen, Rui Zhang, Rui Johansson, Jan Meng, Qing Chen, Gefei |
author_sort | Li, Xue |
collection | PubMed |
description | [Image: see text] Spider flagelliform silk shows the best extensibility among various types of silk, but its biomimetic preparation has not been much studied. Herein, five customized flagelliform spidroins (FlSps: S and NTD(Fl)-Sn-CTD(Fl), n = 1–4), in which the repetitive region (S) and N-/C- terminal domains (NTD(Fl) and CTD(Fl)) are from the same spidroin and spider species, were produced recombinantly. The recombinant spidroins with terminal domains were able to form silk-like fibers with diameters of ∼5 μm by manual pulling at pH 8.0, where the secondary structure transformation occurred. The silk-like fibers from NTD(Fl)-S4-CTD(Fl) showed the highest tensile strength (∼250 MPa), while those ones with 1–3 S broke at a similar stress (∼180 MPa), suggesting that increasing the amounts of the repetitive region can improve the tensile strength, but a certain threshold might need to be reached. This study shows successful preparation of flagelliform silk-like fibers with good mechanical properties, providing general insights into efficient biomimetic preparations of spider silks. |
format | Online Article Text |
id | pubmed-8753598 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-87535982022-01-12 Customized Flagelliform Spidroins Form Spider Silk-like Fibers at pH 8.0 with Outstanding Tensile Strength Li, Xue Qi, Xingmei Cai, Yu-ming Sun, Yuan Wen, Rui Zhang, Rui Johansson, Jan Meng, Qing Chen, Gefei ACS Biomater Sci Eng [Image: see text] Spider flagelliform silk shows the best extensibility among various types of silk, but its biomimetic preparation has not been much studied. Herein, five customized flagelliform spidroins (FlSps: S and NTD(Fl)-Sn-CTD(Fl), n = 1–4), in which the repetitive region (S) and N-/C- terminal domains (NTD(Fl) and CTD(Fl)) are from the same spidroin and spider species, were produced recombinantly. The recombinant spidroins with terminal domains were able to form silk-like fibers with diameters of ∼5 μm by manual pulling at pH 8.0, where the secondary structure transformation occurred. The silk-like fibers from NTD(Fl)-S4-CTD(Fl) showed the highest tensile strength (∼250 MPa), while those ones with 1–3 S broke at a similar stress (∼180 MPa), suggesting that increasing the amounts of the repetitive region can improve the tensile strength, but a certain threshold might need to be reached. This study shows successful preparation of flagelliform silk-like fibers with good mechanical properties, providing general insights into efficient biomimetic preparations of spider silks. American Chemical Society 2021-12-15 2022-01-10 /pmc/articles/PMC8753598/ /pubmed/34908395 http://dx.doi.org/10.1021/acsbiomaterials.1c01354 Text en © 2021 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Li, Xue Qi, Xingmei Cai, Yu-ming Sun, Yuan Wen, Rui Zhang, Rui Johansson, Jan Meng, Qing Chen, Gefei Customized Flagelliform Spidroins Form Spider Silk-like Fibers at pH 8.0 with Outstanding Tensile Strength |
title | Customized Flagelliform Spidroins Form Spider Silk-like
Fibers at pH 8.0 with Outstanding Tensile Strength |
title_full | Customized Flagelliform Spidroins Form Spider Silk-like
Fibers at pH 8.0 with Outstanding Tensile Strength |
title_fullStr | Customized Flagelliform Spidroins Form Spider Silk-like
Fibers at pH 8.0 with Outstanding Tensile Strength |
title_full_unstemmed | Customized Flagelliform Spidroins Form Spider Silk-like
Fibers at pH 8.0 with Outstanding Tensile Strength |
title_short | Customized Flagelliform Spidroins Form Spider Silk-like
Fibers at pH 8.0 with Outstanding Tensile Strength |
title_sort | customized flagelliform spidroins form spider silk-like
fibers at ph 8.0 with outstanding tensile strength |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8753598/ https://www.ncbi.nlm.nih.gov/pubmed/34908395 http://dx.doi.org/10.1021/acsbiomaterials.1c01354 |
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