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Roles of ERRα and TGF-β signaling in stemness enhancement induced by 1 µM bisphenol A exposure via human neural stem cells

Bisphenol A (BPA) is a common industrial chemical widely used to produce various plastics and is known to impair neural stem cells (NSCs). However, the effects of low-dose BPA exposure on the stemness maintenance and differentiation fate of NSCs remain unclear in the infant brain. The present study...

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Autores principales: Dong, Panpan, Ye, Ganghui, Tu, Xinzhuo, Luo, Ying, Cui, Weitong, Ma, Yuxin, Wei, Lei, Tian, Xuewen, Wang, Qinglu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8753968/
https://www.ncbi.nlm.nih.gov/pubmed/35069845
http://dx.doi.org/10.3892/etm.2021.11087
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author Dong, Panpan
Ye, Ganghui
Tu, Xinzhuo
Luo, Ying
Cui, Weitong
Ma, Yuxin
Wei, Lei
Tian, Xuewen
Wang, Qinglu
author_facet Dong, Panpan
Ye, Ganghui
Tu, Xinzhuo
Luo, Ying
Cui, Weitong
Ma, Yuxin
Wei, Lei
Tian, Xuewen
Wang, Qinglu
author_sort Dong, Panpan
collection PubMed
description Bisphenol A (BPA) is a common industrial chemical widely used to produce various plastics and is known to impair neural stem cells (NSCs). However, the effects of low-dose BPA exposure on the stemness maintenance and differentiation fate of NSCs remain unclear in the infant brain. The present study demonstrated that 1 µM BPA promoted human NSC proliferation and stemness, without significantly increasing apoptosis. The Chip-seq experiments demonstrated that both the cell cycle and the TGF-β signaling pathway were accelerated after treatment with 1 µM BPA. Subsequently, estrogen-related receptor α (ERRα) gene knockout cell lines were constructed using CRISPR/Cas9. Further western blotting and chromatin immunoprecipitation-PCR experiments demonstrated that BPA maintained cell stemness by binding to an EERα receptor and activating the TGF-β1 signaling pathway, including the downstream factors Aurora kinases B and Id2. In conclusion, the stemness of NSCs could be maintained by BPA at 1 µM through the activation of the ERRα and TGF-β1 signaling pathways and could restrain the differentiation of NSCs into neurons. The present research further clarified the mechanism of BPA toxicity on NSCs from the novel perspective of ERRα and TGF-β1 signaling pathways regulated by BPA and provided insights into potential novel methods of prevention and therapy for neurogenic diseases.
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spelling pubmed-87539682022-01-21 Roles of ERRα and TGF-β signaling in stemness enhancement induced by 1 µM bisphenol A exposure via human neural stem cells Dong, Panpan Ye, Ganghui Tu, Xinzhuo Luo, Ying Cui, Weitong Ma, Yuxin Wei, Lei Tian, Xuewen Wang, Qinglu Exp Ther Med Articles Bisphenol A (BPA) is a common industrial chemical widely used to produce various plastics and is known to impair neural stem cells (NSCs). However, the effects of low-dose BPA exposure on the stemness maintenance and differentiation fate of NSCs remain unclear in the infant brain. The present study demonstrated that 1 µM BPA promoted human NSC proliferation and stemness, without significantly increasing apoptosis. The Chip-seq experiments demonstrated that both the cell cycle and the TGF-β signaling pathway were accelerated after treatment with 1 µM BPA. Subsequently, estrogen-related receptor α (ERRα) gene knockout cell lines were constructed using CRISPR/Cas9. Further western blotting and chromatin immunoprecipitation-PCR experiments demonstrated that BPA maintained cell stemness by binding to an EERα receptor and activating the TGF-β1 signaling pathway, including the downstream factors Aurora kinases B and Id2. In conclusion, the stemness of NSCs could be maintained by BPA at 1 µM through the activation of the ERRα and TGF-β1 signaling pathways and could restrain the differentiation of NSCs into neurons. The present research further clarified the mechanism of BPA toxicity on NSCs from the novel perspective of ERRα and TGF-β1 signaling pathways regulated by BPA and provided insights into potential novel methods of prevention and therapy for neurogenic diseases. D.A. Spandidos 2022-02 2021-12-22 /pmc/articles/PMC8753968/ /pubmed/35069845 http://dx.doi.org/10.3892/etm.2021.11087 Text en Copyright: © Dong et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Dong, Panpan
Ye, Ganghui
Tu, Xinzhuo
Luo, Ying
Cui, Weitong
Ma, Yuxin
Wei, Lei
Tian, Xuewen
Wang, Qinglu
Roles of ERRα and TGF-β signaling in stemness enhancement induced by 1 µM bisphenol A exposure via human neural stem cells
title Roles of ERRα and TGF-β signaling in stemness enhancement induced by 1 µM bisphenol A exposure via human neural stem cells
title_full Roles of ERRα and TGF-β signaling in stemness enhancement induced by 1 µM bisphenol A exposure via human neural stem cells
title_fullStr Roles of ERRα and TGF-β signaling in stemness enhancement induced by 1 µM bisphenol A exposure via human neural stem cells
title_full_unstemmed Roles of ERRα and TGF-β signaling in stemness enhancement induced by 1 µM bisphenol A exposure via human neural stem cells
title_short Roles of ERRα and TGF-β signaling in stemness enhancement induced by 1 µM bisphenol A exposure via human neural stem cells
title_sort roles of errα and tgf-β signaling in stemness enhancement induced by 1 µm bisphenol a exposure via human neural stem cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8753968/
https://www.ncbi.nlm.nih.gov/pubmed/35069845
http://dx.doi.org/10.3892/etm.2021.11087
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