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RAD51AP2 is required for efficient meiotic recombination between X and Y chromosomes
Faithful segregation of X and Y chromosomes requires meiotic recombination to form a crossover between them in the pseudoautosomal region (PAR). Unlike autosomes that have approximately 10-fold more double-strand breaks (DSBs) than crossovers, one crossover must be formed from the one or two DSBs in...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8754306/ https://www.ncbi.nlm.nih.gov/pubmed/35020426 http://dx.doi.org/10.1126/sciadv.abk1789 |
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author | Ma, Hui Li, Tao Xie, Xuefeng Jiang, Long Ye, Jingwei Gong, Chenjia Jiang, Hanwei Fan, Suixing Zhang, Huan Shi, Baolu Zhang, Beibei Jiang, Xiaohua Li, Yang Zhou, Jianteng Xu, Jianze Zhang, Xingxia Hou, Xiaoning Yin, Hao Zhang, Yuanwei Shi, Qinghua |
author_facet | Ma, Hui Li, Tao Xie, Xuefeng Jiang, Long Ye, Jingwei Gong, Chenjia Jiang, Hanwei Fan, Suixing Zhang, Huan Shi, Baolu Zhang, Beibei Jiang, Xiaohua Li, Yang Zhou, Jianteng Xu, Jianze Zhang, Xingxia Hou, Xiaoning Yin, Hao Zhang, Yuanwei Shi, Qinghua |
author_sort | Ma, Hui |
collection | PubMed |
description | Faithful segregation of X and Y chromosomes requires meiotic recombination to form a crossover between them in the pseudoautosomal region (PAR). Unlike autosomes that have approximately 10-fold more double-strand breaks (DSBs) than crossovers, one crossover must be formed from the one or two DSBs in PARs, implying the existence of a sex chromosome–specific recombination mechanism. Here, we found that RAD51AP2, a meiosis-specific partner of RAD51, is specifically required for the crossover formation on the XY chromosomes, but not autosomes. The decreased crossover formation between X and Y chromosomes in Rad51ap2 mutant mice results from compromised DSB repair in PARs due to destabilization of recombination intermediates rather than defects in DSB generation or synapsis. Our findings provide direct experimental evidence that XY recombination may use a PAR-specific DSB repair mechanism mediated by factors that are not essential for recombination on autosomes. |
format | Online Article Text |
id | pubmed-8754306 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-87543062022-01-27 RAD51AP2 is required for efficient meiotic recombination between X and Y chromosomes Ma, Hui Li, Tao Xie, Xuefeng Jiang, Long Ye, Jingwei Gong, Chenjia Jiang, Hanwei Fan, Suixing Zhang, Huan Shi, Baolu Zhang, Beibei Jiang, Xiaohua Li, Yang Zhou, Jianteng Xu, Jianze Zhang, Xingxia Hou, Xiaoning Yin, Hao Zhang, Yuanwei Shi, Qinghua Sci Adv Biomedicine and Life Sciences Faithful segregation of X and Y chromosomes requires meiotic recombination to form a crossover between them in the pseudoautosomal region (PAR). Unlike autosomes that have approximately 10-fold more double-strand breaks (DSBs) than crossovers, one crossover must be formed from the one or two DSBs in PARs, implying the existence of a sex chromosome–specific recombination mechanism. Here, we found that RAD51AP2, a meiosis-specific partner of RAD51, is specifically required for the crossover formation on the XY chromosomes, but not autosomes. The decreased crossover formation between X and Y chromosomes in Rad51ap2 mutant mice results from compromised DSB repair in PARs due to destabilization of recombination intermediates rather than defects in DSB generation or synapsis. Our findings provide direct experimental evidence that XY recombination may use a PAR-specific DSB repair mechanism mediated by factors that are not essential for recombination on autosomes. American Association for the Advancement of Science 2022-01-12 /pmc/articles/PMC8754306/ /pubmed/35020426 http://dx.doi.org/10.1126/sciadv.abk1789 Text en Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
spellingShingle | Biomedicine and Life Sciences Ma, Hui Li, Tao Xie, Xuefeng Jiang, Long Ye, Jingwei Gong, Chenjia Jiang, Hanwei Fan, Suixing Zhang, Huan Shi, Baolu Zhang, Beibei Jiang, Xiaohua Li, Yang Zhou, Jianteng Xu, Jianze Zhang, Xingxia Hou, Xiaoning Yin, Hao Zhang, Yuanwei Shi, Qinghua RAD51AP2 is required for efficient meiotic recombination between X and Y chromosomes |
title | RAD51AP2 is required for efficient meiotic recombination between X and Y chromosomes |
title_full | RAD51AP2 is required for efficient meiotic recombination between X and Y chromosomes |
title_fullStr | RAD51AP2 is required for efficient meiotic recombination between X and Y chromosomes |
title_full_unstemmed | RAD51AP2 is required for efficient meiotic recombination between X and Y chromosomes |
title_short | RAD51AP2 is required for efficient meiotic recombination between X and Y chromosomes |
title_sort | rad51ap2 is required for efficient meiotic recombination between x and y chromosomes |
topic | Biomedicine and Life Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8754306/ https://www.ncbi.nlm.nih.gov/pubmed/35020426 http://dx.doi.org/10.1126/sciadv.abk1789 |
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