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Calycosin Alleviates Doxorubicin-Induced Cardiotoxicity and Pyroptosis by Inhibiting NLRP3 Inflammasome Activation

Calycosin (CAL) is the main active component present in Astragalus and reportedly possesses diverse pharmacological properties. However, the cardioprotective effect and underlying mechanism of CAL against doxorubicin- (DOX-) induced cardiotoxicity need to be comprehensively examined. Herein, we aime...

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Autores principales: Zhang, Lei, Fan, Cundong, Jiao, Hua-Chen, Zhang, Qian, Jiang, Yue-Hua, Cui, Jie, Liu, Yang, Jiang, Yong-Hao, Zhang, Juan, Yang, Meng-Qi, Li, Yan, Xue, Yi-Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8754606/
https://www.ncbi.nlm.nih.gov/pubmed/35035656
http://dx.doi.org/10.1155/2022/1733834
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author Zhang, Lei
Fan, Cundong
Jiao, Hua-Chen
Zhang, Qian
Jiang, Yue-Hua
Cui, Jie
Liu, Yang
Jiang, Yong-Hao
Zhang, Juan
Yang, Meng-Qi
Li, Yan
Xue, Yi-Tao
author_facet Zhang, Lei
Fan, Cundong
Jiao, Hua-Chen
Zhang, Qian
Jiang, Yue-Hua
Cui, Jie
Liu, Yang
Jiang, Yong-Hao
Zhang, Juan
Yang, Meng-Qi
Li, Yan
Xue, Yi-Tao
author_sort Zhang, Lei
collection PubMed
description Calycosin (CAL) is the main active component present in Astragalus and reportedly possesses diverse pharmacological properties. However, the cardioprotective effect and underlying mechanism of CAL against doxorubicin- (DOX-) induced cardiotoxicity need to be comprehensively examined. Herein, we aimed to investigate whether the cardioprotective effects of CAL are related to its antipyroptotic effect. A cardiatoxicity model was established by stimulating H9c2 cells and C57BL/6J mice using DOX. In vitro, CAL increased H9c2 cell viability and decreased DOX-induced pyroptosis via NLRP3, caspase-1, and gasdermin D signaling pathways in a dose-dependent manner. In vivo, CAL-DOX cotreatment effectively suppressed DOX-induced cytotoxicity as well as inflammatory and cardiomyocyte pyroptosis via the same molecular mechanism. Next, we used nigericin (Nig) and NLRP3 forced overexpression to determine whether CAL imparts antipyroptotic effects by inhibiting the NLRP3 inflammasome in vitro. Furthermore, CAL suppressed DOX-induced mitochondrial oxidative stress injury in H9c2 cells by decreasing the generation of reactive oxygen species and increasing mitochondrial membrane potential and adenosine triphosphate. Likewise, CAL attenuated the DOX-induced increase in malondialdehyde content and decreased superoxide dismutase and glutathione peroxidase activities in H9c2 cells. In vivo, CAL afforded a protective effect against DOX-induced cardiac injury by improving myocardial function, inhibiting brain natriuretic peptide, and improving the changes of the histological morphology of DOX-treated mice. Collectively, our findings confirmed that CAL alleviates DOX-induced cardiotoxicity and pyroptosis by inhibiting NLRP3 inflammasome activation in vivo and in vitro.
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spelling pubmed-87546062022-01-13 Calycosin Alleviates Doxorubicin-Induced Cardiotoxicity and Pyroptosis by Inhibiting NLRP3 Inflammasome Activation Zhang, Lei Fan, Cundong Jiao, Hua-Chen Zhang, Qian Jiang, Yue-Hua Cui, Jie Liu, Yang Jiang, Yong-Hao Zhang, Juan Yang, Meng-Qi Li, Yan Xue, Yi-Tao Oxid Med Cell Longev Research Article Calycosin (CAL) is the main active component present in Astragalus and reportedly possesses diverse pharmacological properties. However, the cardioprotective effect and underlying mechanism of CAL against doxorubicin- (DOX-) induced cardiotoxicity need to be comprehensively examined. Herein, we aimed to investigate whether the cardioprotective effects of CAL are related to its antipyroptotic effect. A cardiatoxicity model was established by stimulating H9c2 cells and C57BL/6J mice using DOX. In vitro, CAL increased H9c2 cell viability and decreased DOX-induced pyroptosis via NLRP3, caspase-1, and gasdermin D signaling pathways in a dose-dependent manner. In vivo, CAL-DOX cotreatment effectively suppressed DOX-induced cytotoxicity as well as inflammatory and cardiomyocyte pyroptosis via the same molecular mechanism. Next, we used nigericin (Nig) and NLRP3 forced overexpression to determine whether CAL imparts antipyroptotic effects by inhibiting the NLRP3 inflammasome in vitro. Furthermore, CAL suppressed DOX-induced mitochondrial oxidative stress injury in H9c2 cells by decreasing the generation of reactive oxygen species and increasing mitochondrial membrane potential and adenosine triphosphate. Likewise, CAL attenuated the DOX-induced increase in malondialdehyde content and decreased superoxide dismutase and glutathione peroxidase activities in H9c2 cells. In vivo, CAL afforded a protective effect against DOX-induced cardiac injury by improving myocardial function, inhibiting brain natriuretic peptide, and improving the changes of the histological morphology of DOX-treated mice. Collectively, our findings confirmed that CAL alleviates DOX-induced cardiotoxicity and pyroptosis by inhibiting NLRP3 inflammasome activation in vivo and in vitro. Hindawi 2022-01-05 /pmc/articles/PMC8754606/ /pubmed/35035656 http://dx.doi.org/10.1155/2022/1733834 Text en Copyright © 2022 Lei Zhang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhang, Lei
Fan, Cundong
Jiao, Hua-Chen
Zhang, Qian
Jiang, Yue-Hua
Cui, Jie
Liu, Yang
Jiang, Yong-Hao
Zhang, Juan
Yang, Meng-Qi
Li, Yan
Xue, Yi-Tao
Calycosin Alleviates Doxorubicin-Induced Cardiotoxicity and Pyroptosis by Inhibiting NLRP3 Inflammasome Activation
title Calycosin Alleviates Doxorubicin-Induced Cardiotoxicity and Pyroptosis by Inhibiting NLRP3 Inflammasome Activation
title_full Calycosin Alleviates Doxorubicin-Induced Cardiotoxicity and Pyroptosis by Inhibiting NLRP3 Inflammasome Activation
title_fullStr Calycosin Alleviates Doxorubicin-Induced Cardiotoxicity and Pyroptosis by Inhibiting NLRP3 Inflammasome Activation
title_full_unstemmed Calycosin Alleviates Doxorubicin-Induced Cardiotoxicity and Pyroptosis by Inhibiting NLRP3 Inflammasome Activation
title_short Calycosin Alleviates Doxorubicin-Induced Cardiotoxicity and Pyroptosis by Inhibiting NLRP3 Inflammasome Activation
title_sort calycosin alleviates doxorubicin-induced cardiotoxicity and pyroptosis by inhibiting nlrp3 inflammasome activation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8754606/
https://www.ncbi.nlm.nih.gov/pubmed/35035656
http://dx.doi.org/10.1155/2022/1733834
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