An integrated rapid nucleic acid detection assay based on recombinant polymerase amplification for SARS-CoV-2

• I-RPA was developed by combining sample treatment and RPA detection in a single sealed cartridge. • No cross reaction was found in respiratory tract-associated viruses. • The low limit of detection was 35 copies/reaction. • This I-RPA assay is suitable for detection and monitoring of SARS-CoV-2 in...

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Autores principales: Tang, Ying, Wang, Yiqin, Li, Yuchang, Zhao, Huai, Zhang, Sen, Zhang, Ying, Li, Jing, Chen, Yuehong, Wu, Xiaoyan, Qin, Chengfeng, Jiang, Tao, Kang, Xiaoping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wuhan Institute of Virology, Chinese Academy of Sciences 2022
Materias:
Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8755414/
https://www.ncbi.nlm.nih.gov/pubmed/35234627
http://dx.doi.org/10.1016/j.virs.2022.01.006
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author Tang, Ying
Wang, Yiqin
Li, Yuchang
Zhao, Huai
Zhang, Sen
Zhang, Ying
Li, Jing
Chen, Yuehong
Wu, Xiaoyan
Qin, Chengfeng
Jiang, Tao
Kang, Xiaoping
author_facet Tang, Ying
Wang, Yiqin
Li, Yuchang
Zhao, Huai
Zhang, Sen
Zhang, Ying
Li, Jing
Chen, Yuehong
Wu, Xiaoyan
Qin, Chengfeng
Jiang, Tao
Kang, Xiaoping
author_sort Tang, Ying
collection PubMed
description • I-RPA was developed by combining sample treatment and RPA detection in a single sealed cartridge. • No cross reaction was found in respiratory tract-associated viruses. • The low limit of detection was 35 copies/reaction. • This I-RPA assay is suitable for detection and monitoring of SARS-CoV-2 in the field.
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spelling pubmed-87554142022-01-13 An integrated rapid nucleic acid detection assay based on recombinant polymerase amplification for SARS-CoV-2 Tang, Ying Wang, Yiqin Li, Yuchang Zhao, Huai Zhang, Sen Zhang, Ying Li, Jing Chen, Yuehong Wu, Xiaoyan Qin, Chengfeng Jiang, Tao Kang, Xiaoping Virol Sin Letter • I-RPA was developed by combining sample treatment and RPA detection in a single sealed cartridge. • No cross reaction was found in respiratory tract-associated viruses. • The low limit of detection was 35 copies/reaction. • This I-RPA assay is suitable for detection and monitoring of SARS-CoV-2 in the field. Wuhan Institute of Virology, Chinese Academy of Sciences 2022-01-13 /pmc/articles/PMC8755414/ /pubmed/35234627 http://dx.doi.org/10.1016/j.virs.2022.01.006 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Letter
Tang, Ying
Wang, Yiqin
Li, Yuchang
Zhao, Huai
Zhang, Sen
Zhang, Ying
Li, Jing
Chen, Yuehong
Wu, Xiaoyan
Qin, Chengfeng
Jiang, Tao
Kang, Xiaoping
An integrated rapid nucleic acid detection assay based on recombinant polymerase amplification for SARS-CoV-2
title An integrated rapid nucleic acid detection assay based on recombinant polymerase amplification for SARS-CoV-2
title_full An integrated rapid nucleic acid detection assay based on recombinant polymerase amplification for SARS-CoV-2
title_fullStr An integrated rapid nucleic acid detection assay based on recombinant polymerase amplification for SARS-CoV-2
title_full_unstemmed An integrated rapid nucleic acid detection assay based on recombinant polymerase amplification for SARS-CoV-2
title_short An integrated rapid nucleic acid detection assay based on recombinant polymerase amplification for SARS-CoV-2
title_sort integrated rapid nucleic acid detection assay based on recombinant polymerase amplification for sars-cov-2
topic Letter
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8755414/
https://www.ncbi.nlm.nih.gov/pubmed/35234627
http://dx.doi.org/10.1016/j.virs.2022.01.006
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