Microfluidic separation of axonal and somal compartments of neural progenitor cells differentiated in a 3D matrix

This protocol describes the differentiation of human neural progenitor cells (hNPCs) in a microfluidic device containing a thin 3D matrix with two separate chambers, enabling a cleaner separation between axons and soma/bulk neurons. We have used this technique to study how mitochondria-associated ER...

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Detalles Bibliográficos
Autores principales: Lotlikar, Madhura S., Tarantino, Marina B., Jorfi, Mehdi, Kovacs, Dora M., Tanzi, Rudolph E., Bhattacharyya, Raja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8755568/
https://www.ncbi.nlm.nih.gov/pubmed/35059649
http://dx.doi.org/10.1016/j.xpro.2021.101028

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