DNA ligase IV mutations confer shorter lifespan and increased sensitivity to nutrient stress in Drosophila melanogaster

The nonhomologous end-joining pathway is a primary DNA double-strand break repair pathway in eukaryotes. DNA ligase IV (Lig4) catalyzes the final step of DNA end ligation in this pathway. Partial loss of Lig4 in mammals causes Lig4 syndrome, while complete loss is embryonically lethal. DNA ligase 4...

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Autores principales: Joshi, Rashmi, Banerjee, Surya Jyoti, Curtiss, Jennifer, Ashley, Amanda K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Animal Genetics • Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8755683/
https://www.ncbi.nlm.nih.gov/pubmed/34817771
http://dx.doi.org/10.1007/s13353-021-00637-0
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author Joshi, Rashmi
Banerjee, Surya Jyoti
Curtiss, Jennifer
Ashley, Amanda K.
author_facet Joshi, Rashmi
Banerjee, Surya Jyoti
Curtiss, Jennifer
Ashley, Amanda K.
author_sort Joshi, Rashmi
collection PubMed
description The nonhomologous end-joining pathway is a primary DNA double-strand break repair pathway in eukaryotes. DNA ligase IV (Lig4) catalyzes the final step of DNA end ligation in this pathway. Partial loss of Lig4 in mammals causes Lig4 syndrome, while complete loss is embryonically lethal. DNA ligase 4 (DNAlig4) null Drosophila melanogaster is viable, but sensitive to ionizing radiation during early development. We proposed to explore if DNAlig4 loss induced other long-term sensitivities and defects in D. melanogaster. We demonstrated that DNAlig4 mutant strains had decreased lifespan and lower resistance to nutrient deprivation, indicating Lig4 is required for maintaining health and longevity in D. melanogaster.
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spelling pubmed-87556832022-01-20 DNA ligase IV mutations confer shorter lifespan and increased sensitivity to nutrient stress in Drosophila melanogaster Joshi, Rashmi Banerjee, Surya Jyoti Curtiss, Jennifer Ashley, Amanda K. J Appl Genet Animal Genetics • Short Communication The nonhomologous end-joining pathway is a primary DNA double-strand break repair pathway in eukaryotes. DNA ligase IV (Lig4) catalyzes the final step of DNA end ligation in this pathway. Partial loss of Lig4 in mammals causes Lig4 syndrome, while complete loss is embryonically lethal. DNA ligase 4 (DNAlig4) null Drosophila melanogaster is viable, but sensitive to ionizing radiation during early development. We proposed to explore if DNAlig4 loss induced other long-term sensitivities and defects in D. melanogaster. We demonstrated that DNAlig4 mutant strains had decreased lifespan and lower resistance to nutrient deprivation, indicating Lig4 is required for maintaining health and longevity in D. melanogaster. Springer Berlin Heidelberg 2021-11-24 2022 /pmc/articles/PMC8755683/ /pubmed/34817771 http://dx.doi.org/10.1007/s13353-021-00637-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Animal Genetics • Short Communication
Joshi, Rashmi
Banerjee, Surya Jyoti
Curtiss, Jennifer
Ashley, Amanda K.
DNA ligase IV mutations confer shorter lifespan and increased sensitivity to nutrient stress in Drosophila melanogaster
title DNA ligase IV mutations confer shorter lifespan and increased sensitivity to nutrient stress in Drosophila melanogaster
title_full DNA ligase IV mutations confer shorter lifespan and increased sensitivity to nutrient stress in Drosophila melanogaster
title_fullStr DNA ligase IV mutations confer shorter lifespan and increased sensitivity to nutrient stress in Drosophila melanogaster
title_full_unstemmed DNA ligase IV mutations confer shorter lifespan and increased sensitivity to nutrient stress in Drosophila melanogaster
title_short DNA ligase IV mutations confer shorter lifespan and increased sensitivity to nutrient stress in Drosophila melanogaster
title_sort dna ligase iv mutations confer shorter lifespan and increased sensitivity to nutrient stress in drosophila melanogaster
topic Animal Genetics • Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8755683/
https://www.ncbi.nlm.nih.gov/pubmed/34817771
http://dx.doi.org/10.1007/s13353-021-00637-0
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