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Quantification of Peptide-Bound Particles: A Phage Mimicking Approach via Site-Selective Immobilization on Glass

[Image: see text] The increasing complexity and need of high-tech materials for modern electronics raise the demand for rare earth elements. While recycling rates are still negligible for most elements, geopolitical tensions, circular economy, and the aim for a carbon-neutral society put pressure on...

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Autores principales: Schrader, Martin, Bobeth, Caroline, Lederer, Franziska L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8756571/
https://www.ncbi.nlm.nih.gov/pubmed/35036690
http://dx.doi.org/10.1021/acsomega.1c04343
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author Schrader, Martin
Bobeth, Caroline
Lederer, Franziska L.
author_facet Schrader, Martin
Bobeth, Caroline
Lederer, Franziska L.
author_sort Schrader, Martin
collection PubMed
description [Image: see text] The increasing complexity and need of high-tech materials for modern electronics raise the demand for rare earth elements. While recycling rates are still negligible for most elements, geopolitical tensions, circular economy, and the aim for a carbon-neutral society put pressure on conventional supply strategies and emphasize the need for new ideas for recycling. Our research group works on the development of phage surface display (PSD)-derived peptide-based recycling methods for electronic waste. This study focuses on LaPO(4):Ce,Tb (LAP), a component of electronic waste from compact energy-saving lamps containing rare earth element-enriched fluorescent powders. While free solution-phase peptides show little to no interaction with the target material, we re-enabled the binding capability by immobilizing them on various glass supports. We shine a spotlight on the transition from phage-bound to free peptides and present the first proof of successful peptide-LAP particle interactions of previously reported PSD-derived sequences. Therefore, we introduce a method to investigate peptide–particle–interactions qualitatively and quantitatively. Additionally, a calibration curve allowed the quantification of peptide-bound particles. Combined with the quantification of the immobilized peptide on the surface, it was possible to calculate a potential dosage of peptides for future recycling processes.
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spelling pubmed-87565712022-01-13 Quantification of Peptide-Bound Particles: A Phage Mimicking Approach via Site-Selective Immobilization on Glass Schrader, Martin Bobeth, Caroline Lederer, Franziska L. ACS Omega [Image: see text] The increasing complexity and need of high-tech materials for modern electronics raise the demand for rare earth elements. While recycling rates are still negligible for most elements, geopolitical tensions, circular economy, and the aim for a carbon-neutral society put pressure on conventional supply strategies and emphasize the need for new ideas for recycling. Our research group works on the development of phage surface display (PSD)-derived peptide-based recycling methods for electronic waste. This study focuses on LaPO(4):Ce,Tb (LAP), a component of electronic waste from compact energy-saving lamps containing rare earth element-enriched fluorescent powders. While free solution-phase peptides show little to no interaction with the target material, we re-enabled the binding capability by immobilizing them on various glass supports. We shine a spotlight on the transition from phage-bound to free peptides and present the first proof of successful peptide-LAP particle interactions of previously reported PSD-derived sequences. Therefore, we introduce a method to investigate peptide–particle–interactions qualitatively and quantitatively. Additionally, a calibration curve allowed the quantification of peptide-bound particles. Combined with the quantification of the immobilized peptide on the surface, it was possible to calculate a potential dosage of peptides for future recycling processes. American Chemical Society 2021-12-20 /pmc/articles/PMC8756571/ /pubmed/35036690 http://dx.doi.org/10.1021/acsomega.1c04343 Text en © 2021 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Schrader, Martin
Bobeth, Caroline
Lederer, Franziska L.
Quantification of Peptide-Bound Particles: A Phage Mimicking Approach via Site-Selective Immobilization on Glass
title Quantification of Peptide-Bound Particles: A Phage Mimicking Approach via Site-Selective Immobilization on Glass
title_full Quantification of Peptide-Bound Particles: A Phage Mimicking Approach via Site-Selective Immobilization on Glass
title_fullStr Quantification of Peptide-Bound Particles: A Phage Mimicking Approach via Site-Selective Immobilization on Glass
title_full_unstemmed Quantification of Peptide-Bound Particles: A Phage Mimicking Approach via Site-Selective Immobilization on Glass
title_short Quantification of Peptide-Bound Particles: A Phage Mimicking Approach via Site-Selective Immobilization on Glass
title_sort quantification of peptide-bound particles: a phage mimicking approach via site-selective immobilization on glass
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8756571/
https://www.ncbi.nlm.nih.gov/pubmed/35036690
http://dx.doi.org/10.1021/acsomega.1c04343
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