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Novel nitrite reductase domain structure suggests a chimeric denitrification repertoire in the phylum Chloroflexi

Denitrification plays a central role in the global nitrogen cycle, reducing and removing nitrogen from marine and terrestrial ecosystems. The flux of nitrogen species through this pathway has a widespread impact, affecting ecological carrying capacity, agriculture, and climate. Nitrite reductase (Ni...

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Autores principales: Schwartz, Sarah L., Momper, Lily, Rangel, Luiz Thiberio, Magnabosco, Cara, Amend, Jan P., Fournier, Gregory P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8756737/
https://www.ncbi.nlm.nih.gov/pubmed/35212484
http://dx.doi.org/10.1002/mbo3.1258
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author Schwartz, Sarah L.
Momper, Lily
Rangel, Luiz Thiberio
Magnabosco, Cara
Amend, Jan P.
Fournier, Gregory P.
author_facet Schwartz, Sarah L.
Momper, Lily
Rangel, Luiz Thiberio
Magnabosco, Cara
Amend, Jan P.
Fournier, Gregory P.
author_sort Schwartz, Sarah L.
collection PubMed
description Denitrification plays a central role in the global nitrogen cycle, reducing and removing nitrogen from marine and terrestrial ecosystems. The flux of nitrogen species through this pathway has a widespread impact, affecting ecological carrying capacity, agriculture, and climate. Nitrite reductase (Nir) and nitric oxide reductase (NOR) are the two central enzymes in this pathway. Here we present a previously unreported Nir domain architecture in members of phylum Chloroflexi. Phylogenetic analyses of protein domains within Nir indicate that an ancestral horizontal transfer and fusion event produced this chimeric domain architecture. We also identify an expanded genomic diversity of a rarely reported NOR subtype, eNOR. Together, these results suggest a greater diversity of denitrification enzyme arrangements exist than have been previously reported.
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spelling pubmed-87567372022-01-19 Novel nitrite reductase domain structure suggests a chimeric denitrification repertoire in the phylum Chloroflexi Schwartz, Sarah L. Momper, Lily Rangel, Luiz Thiberio Magnabosco, Cara Amend, Jan P. Fournier, Gregory P. Microbiologyopen Original Articles Denitrification plays a central role in the global nitrogen cycle, reducing and removing nitrogen from marine and terrestrial ecosystems. The flux of nitrogen species through this pathway has a widespread impact, affecting ecological carrying capacity, agriculture, and climate. Nitrite reductase (Nir) and nitric oxide reductase (NOR) are the two central enzymes in this pathway. Here we present a previously unreported Nir domain architecture in members of phylum Chloroflexi. Phylogenetic analyses of protein domains within Nir indicate that an ancestral horizontal transfer and fusion event produced this chimeric domain architecture. We also identify an expanded genomic diversity of a rarely reported NOR subtype, eNOR. Together, these results suggest a greater diversity of denitrification enzyme arrangements exist than have been previously reported. John Wiley and Sons Inc. 2022-01-13 /pmc/articles/PMC8756737/ /pubmed/35212484 http://dx.doi.org/10.1002/mbo3.1258 Text en © 2021 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Schwartz, Sarah L.
Momper, Lily
Rangel, Luiz Thiberio
Magnabosco, Cara
Amend, Jan P.
Fournier, Gregory P.
Novel nitrite reductase domain structure suggests a chimeric denitrification repertoire in the phylum Chloroflexi
title Novel nitrite reductase domain structure suggests a chimeric denitrification repertoire in the phylum Chloroflexi
title_full Novel nitrite reductase domain structure suggests a chimeric denitrification repertoire in the phylum Chloroflexi
title_fullStr Novel nitrite reductase domain structure suggests a chimeric denitrification repertoire in the phylum Chloroflexi
title_full_unstemmed Novel nitrite reductase domain structure suggests a chimeric denitrification repertoire in the phylum Chloroflexi
title_short Novel nitrite reductase domain structure suggests a chimeric denitrification repertoire in the phylum Chloroflexi
title_sort novel nitrite reductase domain structure suggests a chimeric denitrification repertoire in the phylum chloroflexi
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8756737/
https://www.ncbi.nlm.nih.gov/pubmed/35212484
http://dx.doi.org/10.1002/mbo3.1258
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