Cargando…

Macroscopic Banding Pattern of Collagen Gel Formed by a Diffusion-Reaction Process

[Image: see text] Shapes and patterns observed in internal organs and tissues are reproducibly and robustly produced over a long distance (up to millimeters in length). The most fundamental remaining question is how these long geometries of shape and pattern form arise from the genetic message. Rece...

Descripción completa

Detalles Bibliográficos
Autores principales: Narita, Takayuki, Kondo, Misaki, Oishi, Yushi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8756805/
https://www.ncbi.nlm.nih.gov/pubmed/35036765
http://dx.doi.org/10.1021/acsomega.1c05601
_version_ 1784632636604416000
author Narita, Takayuki
Kondo, Misaki
Oishi, Yushi
author_facet Narita, Takayuki
Kondo, Misaki
Oishi, Yushi
author_sort Narita, Takayuki
collection PubMed
description [Image: see text] Shapes and patterns observed in internal organs and tissues are reproducibly and robustly produced over a long distance (up to millimeters in length). The most fundamental remaining question is how these long geometries of shape and pattern form arise from the genetic message. Recent studies have demonstrated that extracellular matrix (ECM) critically participates as a structural foundation on which cells can organize and communicate. ECMs may be a key to understanding the underlying mechanisms of long-distance patterning and morphogenesis. However, previous studies in this field mainly focused on the complexes and interaction of cells and ECM. This paper pays particular attention to ECM and demonstrates that collagen, a major ECM component, natively possesses the reproducible and definite patterning ability reaching centimeter-scale length. The macroscopic pattern consists of striped transparent layers. The observation under crossed Nicols demonstrates that the layers consist of alternately arranged polarized and unpolarized parts. Confocal fluorescence microscopy studies revealed that the polarized and unpolarized segments include collagen-rich and -poor regions, respectively. The patterning process was proposed based on the Liesegang banding formation, which are mineral precipitation bands formed in hydrogel matrixes. These findings will give hints to the questions about long-distance cell alignment and provide new clues to artificially control cell placement over micron size in the field of regenerative medicine.
format Online
Article
Text
id pubmed-8756805
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-87568052022-01-13 Macroscopic Banding Pattern of Collagen Gel Formed by a Diffusion-Reaction Process Narita, Takayuki Kondo, Misaki Oishi, Yushi ACS Omega [Image: see text] Shapes and patterns observed in internal organs and tissues are reproducibly and robustly produced over a long distance (up to millimeters in length). The most fundamental remaining question is how these long geometries of shape and pattern form arise from the genetic message. Recent studies have demonstrated that extracellular matrix (ECM) critically participates as a structural foundation on which cells can organize and communicate. ECMs may be a key to understanding the underlying mechanisms of long-distance patterning and morphogenesis. However, previous studies in this field mainly focused on the complexes and interaction of cells and ECM. This paper pays particular attention to ECM and demonstrates that collagen, a major ECM component, natively possesses the reproducible and definite patterning ability reaching centimeter-scale length. The macroscopic pattern consists of striped transparent layers. The observation under crossed Nicols demonstrates that the layers consist of alternately arranged polarized and unpolarized parts. Confocal fluorescence microscopy studies revealed that the polarized and unpolarized segments include collagen-rich and -poor regions, respectively. The patterning process was proposed based on the Liesegang banding formation, which are mineral precipitation bands formed in hydrogel matrixes. These findings will give hints to the questions about long-distance cell alignment and provide new clues to artificially control cell placement over micron size in the field of regenerative medicine. American Chemical Society 2021-12-18 /pmc/articles/PMC8756805/ /pubmed/35036765 http://dx.doi.org/10.1021/acsomega.1c05601 Text en © 2021 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Narita, Takayuki
Kondo, Misaki
Oishi, Yushi
Macroscopic Banding Pattern of Collagen Gel Formed by a Diffusion-Reaction Process
title Macroscopic Banding Pattern of Collagen Gel Formed by a Diffusion-Reaction Process
title_full Macroscopic Banding Pattern of Collagen Gel Formed by a Diffusion-Reaction Process
title_fullStr Macroscopic Banding Pattern of Collagen Gel Formed by a Diffusion-Reaction Process
title_full_unstemmed Macroscopic Banding Pattern of Collagen Gel Formed by a Diffusion-Reaction Process
title_short Macroscopic Banding Pattern of Collagen Gel Formed by a Diffusion-Reaction Process
title_sort macroscopic banding pattern of collagen gel formed by a diffusion-reaction process
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8756805/
https://www.ncbi.nlm.nih.gov/pubmed/35036765
http://dx.doi.org/10.1021/acsomega.1c05601
work_keys_str_mv AT naritatakayuki macroscopicbandingpatternofcollagengelformedbyadiffusionreactionprocess
AT kondomisaki macroscopicbandingpatternofcollagengelformedbyadiffusionreactionprocess
AT oishiyushi macroscopicbandingpatternofcollagengelformedbyadiffusionreactionprocess