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Mode-of-Action of Antimicrobial Peptides: Membrane Disruption vs. Intracellular Mechanisms
Antimicrobial peptides are an attractive alternative to traditional antibiotics, due to their physicochemical properties, activity toward a broad spectrum of bacteria, and mode-of-actions distinct from those used by current antibiotics. In general, antimicrobial peptides kill bacteria by either disr...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8757789/ https://www.ncbi.nlm.nih.gov/pubmed/35047892 http://dx.doi.org/10.3389/fmedt.2020.610997 |
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author | Benfield, Aurélie H. Henriques, Sónia Troeira |
author_facet | Benfield, Aurélie H. Henriques, Sónia Troeira |
author_sort | Benfield, Aurélie H. |
collection | PubMed |
description | Antimicrobial peptides are an attractive alternative to traditional antibiotics, due to their physicochemical properties, activity toward a broad spectrum of bacteria, and mode-of-actions distinct from those used by current antibiotics. In general, antimicrobial peptides kill bacteria by either disrupting their membrane, or by entering inside bacterial cells to interact with intracellular components. Characterization of their mode-of-action is essential to improve their activity, avoid resistance in bacterial pathogens, and accelerate their use as therapeutics. Here we review experimental biophysical tools that can be employed with model membranes and bacterial cells to characterize the mode-of-action of antimicrobial peptides. |
format | Online Article Text |
id | pubmed-8757789 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-87577892022-01-18 Mode-of-Action of Antimicrobial Peptides: Membrane Disruption vs. Intracellular Mechanisms Benfield, Aurélie H. Henriques, Sónia Troeira Front Med Technol Medical Technology Antimicrobial peptides are an attractive alternative to traditional antibiotics, due to their physicochemical properties, activity toward a broad spectrum of bacteria, and mode-of-actions distinct from those used by current antibiotics. In general, antimicrobial peptides kill bacteria by either disrupting their membrane, or by entering inside bacterial cells to interact with intracellular components. Characterization of their mode-of-action is essential to improve their activity, avoid resistance in bacterial pathogens, and accelerate their use as therapeutics. Here we review experimental biophysical tools that can be employed with model membranes and bacterial cells to characterize the mode-of-action of antimicrobial peptides. Frontiers Media S.A. 2020-12-11 /pmc/articles/PMC8757789/ /pubmed/35047892 http://dx.doi.org/10.3389/fmedt.2020.610997 Text en Copyright © 2020 Benfield and Henriques. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Medical Technology Benfield, Aurélie H. Henriques, Sónia Troeira Mode-of-Action of Antimicrobial Peptides: Membrane Disruption vs. Intracellular Mechanisms |
title | Mode-of-Action of Antimicrobial Peptides: Membrane Disruption vs. Intracellular Mechanisms |
title_full | Mode-of-Action of Antimicrobial Peptides: Membrane Disruption vs. Intracellular Mechanisms |
title_fullStr | Mode-of-Action of Antimicrobial Peptides: Membrane Disruption vs. Intracellular Mechanisms |
title_full_unstemmed | Mode-of-Action of Antimicrobial Peptides: Membrane Disruption vs. Intracellular Mechanisms |
title_short | Mode-of-Action of Antimicrobial Peptides: Membrane Disruption vs. Intracellular Mechanisms |
title_sort | mode-of-action of antimicrobial peptides: membrane disruption vs. intracellular mechanisms |
topic | Medical Technology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8757789/ https://www.ncbi.nlm.nih.gov/pubmed/35047892 http://dx.doi.org/10.3389/fmedt.2020.610997 |
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