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Enhanced Immune Responses and Protective Immunity to Zika Virus Induced by a DNA Vaccine Encoding a Chimeric NS1 Fused With Type 1 Herpes Virus gD Protein

Zika virus (ZIKV) is a globally-distributed flavivirus transmitted to humans by Aedes mosquitoes, usually causing mild symptoms that may evolve to severe conditions, including neurological alterations, such as neonatal microcephaly and Guillain-Barré syndrome. Due to the absence of specific and effe...

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Autores principales: Pereira, Lennon Ramos, Alves, Rúbens Prince dos Santos, Sales, Natiely Silva, Andreata-Santos, Robert, Venceslau-Carvalho, Aléxia Adrianne, Pereira, Samuel Santos, Castro-Amarante, Maria Fernanda, Rodrigues-Jesus, Mônica Josiane, Favaro, Marianna Teixeira de Pinho, Chura-Chambi, Rosa Maria, Morganti, Ligia, Ferreira, Luís Carlos de Souza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8757838/
https://www.ncbi.nlm.nih.gov/pubmed/35047887
http://dx.doi.org/10.3389/fmedt.2020.604160
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author Pereira, Lennon Ramos
Alves, Rúbens Prince dos Santos
Sales, Natiely Silva
Andreata-Santos, Robert
Venceslau-Carvalho, Aléxia Adrianne
Pereira, Samuel Santos
Castro-Amarante, Maria Fernanda
Rodrigues-Jesus, Mônica Josiane
Favaro, Marianna Teixeira de Pinho
Chura-Chambi, Rosa Maria
Morganti, Ligia
Ferreira, Luís Carlos de Souza
author_facet Pereira, Lennon Ramos
Alves, Rúbens Prince dos Santos
Sales, Natiely Silva
Andreata-Santos, Robert
Venceslau-Carvalho, Aléxia Adrianne
Pereira, Samuel Santos
Castro-Amarante, Maria Fernanda
Rodrigues-Jesus, Mônica Josiane
Favaro, Marianna Teixeira de Pinho
Chura-Chambi, Rosa Maria
Morganti, Ligia
Ferreira, Luís Carlos de Souza
author_sort Pereira, Lennon Ramos
collection PubMed
description Zika virus (ZIKV) is a globally-distributed flavivirus transmitted to humans by Aedes mosquitoes, usually causing mild symptoms that may evolve to severe conditions, including neurological alterations, such as neonatal microcephaly and Guillain-Barré syndrome. Due to the absence of specific and effective preventive methods, we designed a new subunit vaccine based on a DNA vector (pgDNS1-ZIKV) encoding the non-structural protein 1 (NS1) genetically fused to the Herpes Simplex Virus (HSV) glycoprotein D (gD) protein. Recombinant plasmids were replicated in Escherichia coli and the expression of the target protein was confirmed in transfected HEK293 cells. C57BL/6 and AB6 (IFNAR1–/–) mice were i.m. immunized by electroporation in order to evaluate pgDNS1-ZIKV immunogenicity. After two doses, high NS1-specific IgG antibody titers were measured in serum samples collected from pgDNS1-ZIKV-immunized mice. The NS1-specific antibodies were capable to bind the native protein expressed in infected mammalian cells. Immunization with pgDNS1-ZIKV increased both humoral and cellular immune responses regarding mice immunized with a ZIKV NS1 encoding vaccine. Immunization with pgDNS1-ZIKV reduced viremia and morbidity scores leading to enhanced survival of immunodeficient AB6 mice challenged with a lethal virus load. These results give support to the use of ZIKV NS1 as a target antigen and further demonstrate the relevant adjuvant effects of HSV-1 gD.
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spelling pubmed-87578382022-01-18 Enhanced Immune Responses and Protective Immunity to Zika Virus Induced by a DNA Vaccine Encoding a Chimeric NS1 Fused With Type 1 Herpes Virus gD Protein Pereira, Lennon Ramos Alves, Rúbens Prince dos Santos Sales, Natiely Silva Andreata-Santos, Robert Venceslau-Carvalho, Aléxia Adrianne Pereira, Samuel Santos Castro-Amarante, Maria Fernanda Rodrigues-Jesus, Mônica Josiane Favaro, Marianna Teixeira de Pinho Chura-Chambi, Rosa Maria Morganti, Ligia Ferreira, Luís Carlos de Souza Front Med Technol Medical Technology Zika virus (ZIKV) is a globally-distributed flavivirus transmitted to humans by Aedes mosquitoes, usually causing mild symptoms that may evolve to severe conditions, including neurological alterations, such as neonatal microcephaly and Guillain-Barré syndrome. Due to the absence of specific and effective preventive methods, we designed a new subunit vaccine based on a DNA vector (pgDNS1-ZIKV) encoding the non-structural protein 1 (NS1) genetically fused to the Herpes Simplex Virus (HSV) glycoprotein D (gD) protein. Recombinant plasmids were replicated in Escherichia coli and the expression of the target protein was confirmed in transfected HEK293 cells. C57BL/6 and AB6 (IFNAR1–/–) mice were i.m. immunized by electroporation in order to evaluate pgDNS1-ZIKV immunogenicity. After two doses, high NS1-specific IgG antibody titers were measured in serum samples collected from pgDNS1-ZIKV-immunized mice. The NS1-specific antibodies were capable to bind the native protein expressed in infected mammalian cells. Immunization with pgDNS1-ZIKV increased both humoral and cellular immune responses regarding mice immunized with a ZIKV NS1 encoding vaccine. Immunization with pgDNS1-ZIKV reduced viremia and morbidity scores leading to enhanced survival of immunodeficient AB6 mice challenged with a lethal virus load. These results give support to the use of ZIKV NS1 as a target antigen and further demonstrate the relevant adjuvant effects of HSV-1 gD. Frontiers Media S.A. 2020-12-03 /pmc/articles/PMC8757838/ /pubmed/35047887 http://dx.doi.org/10.3389/fmedt.2020.604160 Text en Copyright © 2020 Pereira, Alves, Sales, Andreata-Santos, Venceslau-Carvalho, Pereira, Castro-Amarante, Rodrigues-Jesus, Favaro, Chura-Chambi, Morganti and Ferreira. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Medical Technology
Pereira, Lennon Ramos
Alves, Rúbens Prince dos Santos
Sales, Natiely Silva
Andreata-Santos, Robert
Venceslau-Carvalho, Aléxia Adrianne
Pereira, Samuel Santos
Castro-Amarante, Maria Fernanda
Rodrigues-Jesus, Mônica Josiane
Favaro, Marianna Teixeira de Pinho
Chura-Chambi, Rosa Maria
Morganti, Ligia
Ferreira, Luís Carlos de Souza
Enhanced Immune Responses and Protective Immunity to Zika Virus Induced by a DNA Vaccine Encoding a Chimeric NS1 Fused With Type 1 Herpes Virus gD Protein
title Enhanced Immune Responses and Protective Immunity to Zika Virus Induced by a DNA Vaccine Encoding a Chimeric NS1 Fused With Type 1 Herpes Virus gD Protein
title_full Enhanced Immune Responses and Protective Immunity to Zika Virus Induced by a DNA Vaccine Encoding a Chimeric NS1 Fused With Type 1 Herpes Virus gD Protein
title_fullStr Enhanced Immune Responses and Protective Immunity to Zika Virus Induced by a DNA Vaccine Encoding a Chimeric NS1 Fused With Type 1 Herpes Virus gD Protein
title_full_unstemmed Enhanced Immune Responses and Protective Immunity to Zika Virus Induced by a DNA Vaccine Encoding a Chimeric NS1 Fused With Type 1 Herpes Virus gD Protein
title_short Enhanced Immune Responses and Protective Immunity to Zika Virus Induced by a DNA Vaccine Encoding a Chimeric NS1 Fused With Type 1 Herpes Virus gD Protein
title_sort enhanced immune responses and protective immunity to zika virus induced by a dna vaccine encoding a chimeric ns1 fused with type 1 herpes virus gd protein
topic Medical Technology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8757838/
https://www.ncbi.nlm.nih.gov/pubmed/35047887
http://dx.doi.org/10.3389/fmedt.2020.604160
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