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A short plus long-amplicon based sequencing approach improves genomic coverage and variant detection in the SARS-CoV-2 genome

High viral transmission in the COVID-19 pandemic has enabled SARS‐CoV‐2 to acquire new mutations that may impact genome sequencing methods. The ARTIC.v3 primer pool that amplifies short amplicons in a multiplex-PCR reaction is one of the most widely used methods for sequencing the SARS-CoV-2 genome....

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Detalles Bibliográficos
Autores principales: Arana, Carlos, Liang, Chaoying, Brock, Matthew, Zhang, Bo, Zhou, Jinchun, Chen, Li, Cantarel, Brandi, SoRelle, Jeffrey, Hooper, Lora V., Raj, Prithvi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8757904/
https://www.ncbi.nlm.nih.gov/pubmed/35025877
http://dx.doi.org/10.1371/journal.pone.0261014

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