An international, interlaboratory ring trial confirms the feasibility of an extraction-less “direct” RT-qPCR method for reliable detection of SARS-CoV-2 RNA in clinical samples

Reverse transcription–quantitative polymerase chain reaction (RT-qPCR) is used worldwide to test and trace the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). “Extraction-less” or “direct” real time–reverse transcription polymerase chain reaction (RT-PCR) is a transparent and...

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Autores principales: Mills, Margaret G., Bruce, Emily, Huang, Meei-Li, Crothers, Jessica W., Hyrien, Ollivier, Oura, Christopher A. L., Blake, Lemar, Brown Jordan, Arianne, Hester, Susan, Wehmas, Leah, Mari, Bernard, Barby, Pascal, Lacoux, Caroline, Fassy, Julien, Vial, Pablo, Vial, Cecilia, Martinez, Jose R. W., Oladipo, Olusola Olalekan, Inuwa, Bitrus, Shittu, Ismaila, Meseko, Clement A., Chammas, Roger, Santos, Carlos Ferreira, Dionísio, Thiago José, Garbieri, Thais Francini, Parisi, Viviane Aparecida, Mendes-Correa, Maria Cassia, de Paula, Anderson V., Romano, Camila M., Góes, Luiz Gustavo Bentim, Minoprio, Paola, Campos, Angelica C., Cunha, Marielton P., Vilela, Ana Paula P., Nyirenda, Tonney, Mkakosya, Rajhab Sawasawa, Muula, Adamson S., Dumm, Rebekah E., Harris, Rebecca M., Mitchell, Constance A., Pettit, Syril, Botten, Jason, Jerome, Keith R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8758094/
https://www.ncbi.nlm.nih.gov/pubmed/35025926
http://dx.doi.org/10.1371/journal.pone.0261853

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