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Identification of DNA Repair-Related Genes Predicting Clinical Outcome for Thyroid Cancer
Recent studies have demonstrated the utility and superiority of DNA repair-related genes as novel biomarkers for cancer diagnosis, prognosis, and therapy. Here, we aimed to screen the potential survival-related DNA repair-related genes in thyroid cancer (TC). TCGA datasets were utilized to analyze t...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8758253/ https://www.ncbi.nlm.nih.gov/pubmed/35035484 http://dx.doi.org/10.1155/2022/8809469 |
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author | Zhang, Ai-ying Li, Wei Zhou, Hai-yan Chen, Jing Zhang, Li-bin |
author_facet | Zhang, Ai-ying Li, Wei Zhou, Hai-yan Chen, Jing Zhang, Li-bin |
author_sort | Zhang, Ai-ying |
collection | PubMed |
description | Recent studies have demonstrated the utility and superiority of DNA repair-related genes as novel biomarkers for cancer diagnosis, prognosis, and therapy. Here, we aimed to screen the potential survival-related DNA repair-related genes in thyroid cancer (TC). TCGA datasets were utilized to analyze the differentially expressed DNA repair-related genes between TC and nontumor tissues. The K–M approach and univariate analysis were employed to screen survival-related genes. RT-PCR was employed to examine the expression of DNA repair-related genes in TC samples and matched noncancer samples. CCK-8 analyses were used to determine cellular proliferation. Herein, our team discovered that the expression of four DNA repair-related genes was remarkably upregulated in TC samples in contrast to noncancer samples. Survival assays identified 14 DNA repair-related genes. In our cohort, we observed that the expression of TAF13 and DCTN4 was distinctly elevated in TC specimens in contrast to nontumor specimens. Moreover, knockdown of TAF13 and DCTN4 was observed to inhibit the TC cellular proliferation. Overall, the upregulation of TAF13 and DCTN4 is related to decreased overall survival in TC patients. Therefore, the assessment of TAF13 and DCTN4 expression may be useful for predicting prognosis in these patients. |
format | Online Article Text |
id | pubmed-8758253 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-87582532022-01-14 Identification of DNA Repair-Related Genes Predicting Clinical Outcome for Thyroid Cancer Zhang, Ai-ying Li, Wei Zhou, Hai-yan Chen, Jing Zhang, Li-bin J Oncol Research Article Recent studies have demonstrated the utility and superiority of DNA repair-related genes as novel biomarkers for cancer diagnosis, prognosis, and therapy. Here, we aimed to screen the potential survival-related DNA repair-related genes in thyroid cancer (TC). TCGA datasets were utilized to analyze the differentially expressed DNA repair-related genes between TC and nontumor tissues. The K–M approach and univariate analysis were employed to screen survival-related genes. RT-PCR was employed to examine the expression of DNA repair-related genes in TC samples and matched noncancer samples. CCK-8 analyses were used to determine cellular proliferation. Herein, our team discovered that the expression of four DNA repair-related genes was remarkably upregulated in TC samples in contrast to noncancer samples. Survival assays identified 14 DNA repair-related genes. In our cohort, we observed that the expression of TAF13 and DCTN4 was distinctly elevated in TC specimens in contrast to nontumor specimens. Moreover, knockdown of TAF13 and DCTN4 was observed to inhibit the TC cellular proliferation. Overall, the upregulation of TAF13 and DCTN4 is related to decreased overall survival in TC patients. Therefore, the assessment of TAF13 and DCTN4 expression may be useful for predicting prognosis in these patients. Hindawi 2022-01-06 /pmc/articles/PMC8758253/ /pubmed/35035484 http://dx.doi.org/10.1155/2022/8809469 Text en Copyright © 2022 Ai-ying Zhang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Zhang, Ai-ying Li, Wei Zhou, Hai-yan Chen, Jing Zhang, Li-bin Identification of DNA Repair-Related Genes Predicting Clinical Outcome for Thyroid Cancer |
title | Identification of DNA Repair-Related Genes Predicting Clinical Outcome for Thyroid Cancer |
title_full | Identification of DNA Repair-Related Genes Predicting Clinical Outcome for Thyroid Cancer |
title_fullStr | Identification of DNA Repair-Related Genes Predicting Clinical Outcome for Thyroid Cancer |
title_full_unstemmed | Identification of DNA Repair-Related Genes Predicting Clinical Outcome for Thyroid Cancer |
title_short | Identification of DNA Repair-Related Genes Predicting Clinical Outcome for Thyroid Cancer |
title_sort | identification of dna repair-related genes predicting clinical outcome for thyroid cancer |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8758253/ https://www.ncbi.nlm.nih.gov/pubmed/35035484 http://dx.doi.org/10.1155/2022/8809469 |
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