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Effects and Mechanism of Salvianolic Acid B on the Injury of Human Renal Tubular Epithelial Cells Induced by Iopromide

With the increasing application of medical imaging contrast materials, contrast-induced nephropathy (CIN) has become the third major cause of iatrogenic renal insufficiency. CIN is defined as an absolute increase in serum creatinine levels of at least 0.50 mg/dl or an increase >25% of serum creat...

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Autores principales: Dong, Shu-Jun, Gao, Xin-Yue, Pei, Ming-Xin, Luo, Ting, Fan, Dong, Chen, Yan-Ling, Jin, Jun-Feng, Zhao, Xiao-Duo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8758562/
https://www.ncbi.nlm.nih.gov/pubmed/35035354
http://dx.doi.org/10.3389/fphar.2021.761908
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author Dong, Shu-Jun
Gao, Xin-Yue
Pei, Ming-Xin
Luo, Ting
Fan, Dong
Chen, Yan-Ling
Jin, Jun-Feng
Zhao, Xiao-Duo
author_facet Dong, Shu-Jun
Gao, Xin-Yue
Pei, Ming-Xin
Luo, Ting
Fan, Dong
Chen, Yan-Ling
Jin, Jun-Feng
Zhao, Xiao-Duo
author_sort Dong, Shu-Jun
collection PubMed
description With the increasing application of medical imaging contrast materials, contrast-induced nephropathy (CIN) has become the third major cause of iatrogenic renal insufficiency. CIN is defined as an absolute increase in serum creatinine levels of at least 0.50 mg/dl or an increase >25% of serum creatinine from baseline after exposure to contrast. In this study, the protective effects of salvianolic acid B (Sal B) were detected in human renal tubular epithelial cells (HK-2) exposed to iopromide. The results showed that different concentrations of Sal B counteract the loss of cell viability induced by iopromide, and reduce cell apoptosis, the reactive oxygen species (ROS) levels, and the levels of endoplasmic reticulum stress (ERS)–related and apoptosis-related proteins such as p-IRE-1α, p-eIF-2α/eIF-2α, p-JNK, CHOP, Bax/Bcl-2, and cleaved caspase-3. In addition, Sal B at a concentration of 100 μmol/L inhibited ERS and reduced cell damage to a similar extent as the ERS inhibitor 4-PBA. Importantly, treatment with Sal B could abolish the injury induced by ERS agonist tunicamycin, increasing cell viability and the mitochondrial membrane potential, as well as significantly reducing ROS levels and the expression of Bax/Bcl-2, cleaved-caspase-3, GRP78, p-eIF2α, p-JNK, and CHOP. These results suggested that the protective effect of Sal B against HK-2 cell injury induced by iopromide may be related to the inhibition of ERS.
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spelling pubmed-87585622022-01-15 Effects and Mechanism of Salvianolic Acid B on the Injury of Human Renal Tubular Epithelial Cells Induced by Iopromide Dong, Shu-Jun Gao, Xin-Yue Pei, Ming-Xin Luo, Ting Fan, Dong Chen, Yan-Ling Jin, Jun-Feng Zhao, Xiao-Duo Front Pharmacol Pharmacology With the increasing application of medical imaging contrast materials, contrast-induced nephropathy (CIN) has become the third major cause of iatrogenic renal insufficiency. CIN is defined as an absolute increase in serum creatinine levels of at least 0.50 mg/dl or an increase >25% of serum creatinine from baseline after exposure to contrast. In this study, the protective effects of salvianolic acid B (Sal B) were detected in human renal tubular epithelial cells (HK-2) exposed to iopromide. The results showed that different concentrations of Sal B counteract the loss of cell viability induced by iopromide, and reduce cell apoptosis, the reactive oxygen species (ROS) levels, and the levels of endoplasmic reticulum stress (ERS)–related and apoptosis-related proteins such as p-IRE-1α, p-eIF-2α/eIF-2α, p-JNK, CHOP, Bax/Bcl-2, and cleaved caspase-3. In addition, Sal B at a concentration of 100 μmol/L inhibited ERS and reduced cell damage to a similar extent as the ERS inhibitor 4-PBA. Importantly, treatment with Sal B could abolish the injury induced by ERS agonist tunicamycin, increasing cell viability and the mitochondrial membrane potential, as well as significantly reducing ROS levels and the expression of Bax/Bcl-2, cleaved-caspase-3, GRP78, p-eIF2α, p-JNK, and CHOP. These results suggested that the protective effect of Sal B against HK-2 cell injury induced by iopromide may be related to the inhibition of ERS. Frontiers Media S.A. 2021-12-31 /pmc/articles/PMC8758562/ /pubmed/35035354 http://dx.doi.org/10.3389/fphar.2021.761908 Text en Copyright © 2021 Dong, Gao, Pei, Luo, Fan, Chen, Jin and Zhao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Dong, Shu-Jun
Gao, Xin-Yue
Pei, Ming-Xin
Luo, Ting
Fan, Dong
Chen, Yan-Ling
Jin, Jun-Feng
Zhao, Xiao-Duo
Effects and Mechanism of Salvianolic Acid B on the Injury of Human Renal Tubular Epithelial Cells Induced by Iopromide
title Effects and Mechanism of Salvianolic Acid B on the Injury of Human Renal Tubular Epithelial Cells Induced by Iopromide
title_full Effects and Mechanism of Salvianolic Acid B on the Injury of Human Renal Tubular Epithelial Cells Induced by Iopromide
title_fullStr Effects and Mechanism of Salvianolic Acid B on the Injury of Human Renal Tubular Epithelial Cells Induced by Iopromide
title_full_unstemmed Effects and Mechanism of Salvianolic Acid B on the Injury of Human Renal Tubular Epithelial Cells Induced by Iopromide
title_short Effects and Mechanism of Salvianolic Acid B on the Injury of Human Renal Tubular Epithelial Cells Induced by Iopromide
title_sort effects and mechanism of salvianolic acid b on the injury of human renal tubular epithelial cells induced by iopromide
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8758562/
https://www.ncbi.nlm.nih.gov/pubmed/35035354
http://dx.doi.org/10.3389/fphar.2021.761908
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