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Dysfunction of low-density neutrophils in peripheral circulation in patients with sepsis

Low-density neutrophils (LDNs) have been described in tumors and various autoimmune diseases, where they exhibit immune dysfunction and alter disease progression. Nevertheless, LDNs have been rarely reported in sepsis. We studied sepsis patients admitted to the intensive care unit. Wright-Giemsa sta...

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Autores principales: Sun, Ran, Huang, Jiamin, Yang, Yunxi, Liu, Lu, Shao, Yiming, Li, Linbin, Sun, Bingwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8758723/
https://www.ncbi.nlm.nih.gov/pubmed/35027618
http://dx.doi.org/10.1038/s41598-021-04682-x
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author Sun, Ran
Huang, Jiamin
Yang, Yunxi
Liu, Lu
Shao, Yiming
Li, Linbin
Sun, Bingwei
author_facet Sun, Ran
Huang, Jiamin
Yang, Yunxi
Liu, Lu
Shao, Yiming
Li, Linbin
Sun, Bingwei
author_sort Sun, Ran
collection PubMed
description Low-density neutrophils (LDNs) have been described in tumors and various autoimmune diseases, where they exhibit immune dysfunction and alter disease progression. Nevertheless, LDNs have been rarely reported in sepsis. We studied sepsis patients admitted to the intensive care unit. Wright-Giemsa stain assay and Transmission electron microscopy were performed to detect the morphology of neutrophils. Flow cytometry was used to analyze the number and function of LDNs. Concentration of cytokines was measured using ELISA. Neutrophil chemotaxis was examined using an under-agarose chemotaxis model. We found that LDNs were significantly elevated in patients with sepsis. Phenotypes and morphological characteristics suggest that LDNs may be formed by mixtures of neutrophils at various maturation stages. In vitro experiments showed that LDN formation was closely associated with neutrophil degranulation. We preliminarily discussed changes in immune function in LDNs. Compared with high-density neutrophils, expression levels of CXC chemokine receptor 4 on LDN surfaces were increased, phagocytotic capacity was decreased, and life span was prolonged. The chemotactic ability of LDNs was significantly reduced, possibly related to the increased expression of P2X1. These data suggest that LDNs are essential components of neutrophils in sepsis. To clarify the source and dysfunction mechanism of LDN in sepsis may be helpful for the diagnosis and treatment of sepsis in the future.
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spelling pubmed-87587232022-01-14 Dysfunction of low-density neutrophils in peripheral circulation in patients with sepsis Sun, Ran Huang, Jiamin Yang, Yunxi Liu, Lu Shao, Yiming Li, Linbin Sun, Bingwei Sci Rep Article Low-density neutrophils (LDNs) have been described in tumors and various autoimmune diseases, where they exhibit immune dysfunction and alter disease progression. Nevertheless, LDNs have been rarely reported in sepsis. We studied sepsis patients admitted to the intensive care unit. Wright-Giemsa stain assay and Transmission electron microscopy were performed to detect the morphology of neutrophils. Flow cytometry was used to analyze the number and function of LDNs. Concentration of cytokines was measured using ELISA. Neutrophil chemotaxis was examined using an under-agarose chemotaxis model. We found that LDNs were significantly elevated in patients with sepsis. Phenotypes and morphological characteristics suggest that LDNs may be formed by mixtures of neutrophils at various maturation stages. In vitro experiments showed that LDN formation was closely associated with neutrophil degranulation. We preliminarily discussed changes in immune function in LDNs. Compared with high-density neutrophils, expression levels of CXC chemokine receptor 4 on LDN surfaces were increased, phagocytotic capacity was decreased, and life span was prolonged. The chemotactic ability of LDNs was significantly reduced, possibly related to the increased expression of P2X1. These data suggest that LDNs are essential components of neutrophils in sepsis. To clarify the source and dysfunction mechanism of LDN in sepsis may be helpful for the diagnosis and treatment of sepsis in the future. Nature Publishing Group UK 2022-01-13 /pmc/articles/PMC8758723/ /pubmed/35027618 http://dx.doi.org/10.1038/s41598-021-04682-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Sun, Ran
Huang, Jiamin
Yang, Yunxi
Liu, Lu
Shao, Yiming
Li, Linbin
Sun, Bingwei
Dysfunction of low-density neutrophils in peripheral circulation in patients with sepsis
title Dysfunction of low-density neutrophils in peripheral circulation in patients with sepsis
title_full Dysfunction of low-density neutrophils in peripheral circulation in patients with sepsis
title_fullStr Dysfunction of low-density neutrophils in peripheral circulation in patients with sepsis
title_full_unstemmed Dysfunction of low-density neutrophils in peripheral circulation in patients with sepsis
title_short Dysfunction of low-density neutrophils in peripheral circulation in patients with sepsis
title_sort dysfunction of low-density neutrophils in peripheral circulation in patients with sepsis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8758723/
https://www.ncbi.nlm.nih.gov/pubmed/35027618
http://dx.doi.org/10.1038/s41598-021-04682-x
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