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Differential proteomic analysis demonstrates follicle fluid participate immune reaction and protein translation in yak

BACKGROUND: Ovarian follicle fluid (FF) as a microenvironment surrounding oocyte plays critical roles in physio-biochemical processes of follicle development and oocyte maturation. It is hypothesized that proteins in yak FF participate in the physio-biochemical pathways. The primary aims of this stu...

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Autores principales: Pei, Jie, Song, Rende, Bao, Pengjia, Yin, Mancai, Li, Jiye, Zhang, Guomo, Wu, Fude, Luo, Zhengjie, Wu, Xiaoyun, Song, Weiru, Ba, Yang, Xiong, Lin, Liang, Chunnian, Guo, Xian, Yan, Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8758897/
https://www.ncbi.nlm.nih.gov/pubmed/35031034
http://dx.doi.org/10.1186/s12917-021-03097-0
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author Pei, Jie
Song, Rende
Bao, Pengjia
Yin, Mancai
Li, Jiye
Zhang, Guomo
Wu, Fude
Luo, Zhengjie
Wu, Xiaoyun
Song, Weiru
Ba, Yang
Xiong, Lin
Liang, Chunnian
Guo, Xian
Yan, Ping
author_facet Pei, Jie
Song, Rende
Bao, Pengjia
Yin, Mancai
Li, Jiye
Zhang, Guomo
Wu, Fude
Luo, Zhengjie
Wu, Xiaoyun
Song, Weiru
Ba, Yang
Xiong, Lin
Liang, Chunnian
Guo, Xian
Yan, Ping
author_sort Pei, Jie
collection PubMed
description BACKGROUND: Ovarian follicle fluid (FF) as a microenvironment surrounding oocyte plays critical roles in physio-biochemical processes of follicle development and oocyte maturation. It is hypothesized that proteins in yak FF participate in the physio-biochemical pathways. The primary aims of this study were to find differentially expressed proteins (DEPs) between mature and immature FF, and to elucidating functions of the mature and immature FF in yak. RESULTS: The mature and immature FF samples were obtained from three healthy yaks that were nonpregnant, aged from four to five years, and free from any anatomical reproductive disorders. The FF samples were subjected to mass spectrometry with the isobaric tags for relative and absolute quantification (iTRAQ). The FF samples went through correlation analysis, principle component analysis, and expression pattern analysis based on quantification of the identified proteins. Four hundred sixty-three DEPs between mature and immature FF were identified. The DEPs between the mature and immature FF samples underwent gene ontology (GO), Kyoto encyclopedia of genes and genomes (KEGG), and protein-protein interaction (PPI) analysis. The DEPs highly expressed in the mature FF mainly took parts in the complement and coagulation cascades, defense response, acute-phase response, response to other organism pathways to avoid invasion of exogenous microorganisms. The complement activation pathway contains eight DEPs, namely C2, C5, C6, C7, C9, C4BPA, CFH, and MBL2. The three DEPs, CATHL4, CHGA, and PGLYRP1, take parts in defense response pathway to prevent invasion of exogenetic microorganism. The coagulation cascades pathway involves many coagulation factors, such as F7, F13A1, FGA, FGB, FGG, KLKB1, KNG1, MASP1, SERPINA1, and SERPIND1. While the DEPs highly expressed in the immature FF participated in protein translation, peptide biosynthetic process, DNA conformation change, and DNA geometric change pathways to facilitate follicle development. The translation pathway contains many ribosomal proteins, such as RPL3, RPL5, RPS3, RPS6, and other translation factors, such as EIF3J, EIF4G2, ETF1, MOV10, and NARS. The DNA conformation change and DNA geometric change involve nine DEPs, DDX1, G3BP1, HMGB1, HMGB2, HMGB3, MCM3, MCM5, MCM6, and RUVBL2. Furthermore, the expressed levels of the main DEPs, C2 and SERPIND1, were confirmed by western blot. CONCLUSIONS: The differential proteomics revealed the up-regulated DEPs in mature FF take parts in immunoreaction to prevent invasion of microorganisms and the up-regulated DEPs in immature FF participate in protein synthesis, which may improve our knowledge of the follicular microenvironment and its biological roles for reproductive processes in yak. The DEPs, C2 and SERPIND1, can be considered as protein markers for mature yak follicle. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12917-021-03097-0.
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spelling pubmed-87588972022-01-14 Differential proteomic analysis demonstrates follicle fluid participate immune reaction and protein translation in yak Pei, Jie Song, Rende Bao, Pengjia Yin, Mancai Li, Jiye Zhang, Guomo Wu, Fude Luo, Zhengjie Wu, Xiaoyun Song, Weiru Ba, Yang Xiong, Lin Liang, Chunnian Guo, Xian Yan, Ping BMC Vet Res Research BACKGROUND: Ovarian follicle fluid (FF) as a microenvironment surrounding oocyte plays critical roles in physio-biochemical processes of follicle development and oocyte maturation. It is hypothesized that proteins in yak FF participate in the physio-biochemical pathways. The primary aims of this study were to find differentially expressed proteins (DEPs) between mature and immature FF, and to elucidating functions of the mature and immature FF in yak. RESULTS: The mature and immature FF samples were obtained from three healthy yaks that were nonpregnant, aged from four to five years, and free from any anatomical reproductive disorders. The FF samples were subjected to mass spectrometry with the isobaric tags for relative and absolute quantification (iTRAQ). The FF samples went through correlation analysis, principle component analysis, and expression pattern analysis based on quantification of the identified proteins. Four hundred sixty-three DEPs between mature and immature FF were identified. The DEPs between the mature and immature FF samples underwent gene ontology (GO), Kyoto encyclopedia of genes and genomes (KEGG), and protein-protein interaction (PPI) analysis. The DEPs highly expressed in the mature FF mainly took parts in the complement and coagulation cascades, defense response, acute-phase response, response to other organism pathways to avoid invasion of exogenous microorganisms. The complement activation pathway contains eight DEPs, namely C2, C5, C6, C7, C9, C4BPA, CFH, and MBL2. The three DEPs, CATHL4, CHGA, and PGLYRP1, take parts in defense response pathway to prevent invasion of exogenetic microorganism. The coagulation cascades pathway involves many coagulation factors, such as F7, F13A1, FGA, FGB, FGG, KLKB1, KNG1, MASP1, SERPINA1, and SERPIND1. While the DEPs highly expressed in the immature FF participated in protein translation, peptide biosynthetic process, DNA conformation change, and DNA geometric change pathways to facilitate follicle development. The translation pathway contains many ribosomal proteins, such as RPL3, RPL5, RPS3, RPS6, and other translation factors, such as EIF3J, EIF4G2, ETF1, MOV10, and NARS. The DNA conformation change and DNA geometric change involve nine DEPs, DDX1, G3BP1, HMGB1, HMGB2, HMGB3, MCM3, MCM5, MCM6, and RUVBL2. Furthermore, the expressed levels of the main DEPs, C2 and SERPIND1, were confirmed by western blot. CONCLUSIONS: The differential proteomics revealed the up-regulated DEPs in mature FF take parts in immunoreaction to prevent invasion of microorganisms and the up-regulated DEPs in immature FF participate in protein synthesis, which may improve our knowledge of the follicular microenvironment and its biological roles for reproductive processes in yak. The DEPs, C2 and SERPIND1, can be considered as protein markers for mature yak follicle. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12917-021-03097-0. BioMed Central 2022-01-14 /pmc/articles/PMC8758897/ /pubmed/35031034 http://dx.doi.org/10.1186/s12917-021-03097-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Pei, Jie
Song, Rende
Bao, Pengjia
Yin, Mancai
Li, Jiye
Zhang, Guomo
Wu, Fude
Luo, Zhengjie
Wu, Xiaoyun
Song, Weiru
Ba, Yang
Xiong, Lin
Liang, Chunnian
Guo, Xian
Yan, Ping
Differential proteomic analysis demonstrates follicle fluid participate immune reaction and protein translation in yak
title Differential proteomic analysis demonstrates follicle fluid participate immune reaction and protein translation in yak
title_full Differential proteomic analysis demonstrates follicle fluid participate immune reaction and protein translation in yak
title_fullStr Differential proteomic analysis demonstrates follicle fluid participate immune reaction and protein translation in yak
title_full_unstemmed Differential proteomic analysis demonstrates follicle fluid participate immune reaction and protein translation in yak
title_short Differential proteomic analysis demonstrates follicle fluid participate immune reaction and protein translation in yak
title_sort differential proteomic analysis demonstrates follicle fluid participate immune reaction and protein translation in yak
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8758897/
https://www.ncbi.nlm.nih.gov/pubmed/35031034
http://dx.doi.org/10.1186/s12917-021-03097-0
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