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Making neurons, made easy: The use of Neurogenin-2 in neuronal differentiation
Directed neuronal differentiation of human pluripotent stem cells (hPSCs), neural progenitors, or fibroblasts using transcription factors has allowed for the rapid and highly reproducible differentiation of mature and functional neurons. Exogenous expression of the transcription factor Neurogenin-2...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8758946/ https://www.ncbi.nlm.nih.gov/pubmed/34971564 http://dx.doi.org/10.1016/j.stemcr.2021.11.015 |
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author | Hulme, Amy J. Maksour, Simon St-Clair Glover, Mitchell Miellet, Sara Dottori, Mirella |
author_facet | Hulme, Amy J. Maksour, Simon St-Clair Glover, Mitchell Miellet, Sara Dottori, Mirella |
author_sort | Hulme, Amy J. |
collection | PubMed |
description | Directed neuronal differentiation of human pluripotent stem cells (hPSCs), neural progenitors, or fibroblasts using transcription factors has allowed for the rapid and highly reproducible differentiation of mature and functional neurons. Exogenous expression of the transcription factor Neurogenin-2 (NGN2) has been widely used to generate different populations of neurons, which have been used in neurodevelopment studies, disease modeling, drug screening, and neuronal replacement therapies. Could NGN2 be a “one-glove-fits-all” approach for neuronal differentiations? This review summarizes the cellular roles of NGN2 and describes the applications and limitations of using NGN2 for the rapid and directed differentiation of neurons. |
format | Online Article Text |
id | pubmed-8758946 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-87589462022-01-19 Making neurons, made easy: The use of Neurogenin-2 in neuronal differentiation Hulme, Amy J. Maksour, Simon St-Clair Glover, Mitchell Miellet, Sara Dottori, Mirella Stem Cell Reports Review Directed neuronal differentiation of human pluripotent stem cells (hPSCs), neural progenitors, or fibroblasts using transcription factors has allowed for the rapid and highly reproducible differentiation of mature and functional neurons. Exogenous expression of the transcription factor Neurogenin-2 (NGN2) has been widely used to generate different populations of neurons, which have been used in neurodevelopment studies, disease modeling, drug screening, and neuronal replacement therapies. Could NGN2 be a “one-glove-fits-all” approach for neuronal differentiations? This review summarizes the cellular roles of NGN2 and describes the applications and limitations of using NGN2 for the rapid and directed differentiation of neurons. Elsevier 2021-12-30 /pmc/articles/PMC8758946/ /pubmed/34971564 http://dx.doi.org/10.1016/j.stemcr.2021.11.015 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Review Hulme, Amy J. Maksour, Simon St-Clair Glover, Mitchell Miellet, Sara Dottori, Mirella Making neurons, made easy: The use of Neurogenin-2 in neuronal differentiation |
title | Making neurons, made easy: The use of Neurogenin-2 in neuronal differentiation |
title_full | Making neurons, made easy: The use of Neurogenin-2 in neuronal differentiation |
title_fullStr | Making neurons, made easy: The use of Neurogenin-2 in neuronal differentiation |
title_full_unstemmed | Making neurons, made easy: The use of Neurogenin-2 in neuronal differentiation |
title_short | Making neurons, made easy: The use of Neurogenin-2 in neuronal differentiation |
title_sort | making neurons, made easy: the use of neurogenin-2 in neuronal differentiation |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8758946/ https://www.ncbi.nlm.nih.gov/pubmed/34971564 http://dx.doi.org/10.1016/j.stemcr.2021.11.015 |
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