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EjRAV1/2 Delay Flowering Through Transcriptional Repression of EjFTs and EjSOC1s in Loquat
Most species in Rosaceae usually need to undergo several years of juvenile phase before the initiation of flowering. After 4–6 years’ juvenile phase, cultivated loquat (Eriobotrya japonica), a species in Rosaceae, enters the reproductive phase, blooms in the autumn and sets fruits during the winter....
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8759039/ https://www.ncbi.nlm.nih.gov/pubmed/35035390 http://dx.doi.org/10.3389/fpls.2021.816086 |
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author | Peng, Ze Wang, Man Zhang, Ling Jiang, Yuanyuan Zhao, Chongbin Shahid, Muhammad Qasim Bai, Yunlu Hao, Jingjing Peng, Jiangrong Gao, Yongshun Su, Wenbing Yang, Xianghui |
author_facet | Peng, Ze Wang, Man Zhang, Ling Jiang, Yuanyuan Zhao, Chongbin Shahid, Muhammad Qasim Bai, Yunlu Hao, Jingjing Peng, Jiangrong Gao, Yongshun Su, Wenbing Yang, Xianghui |
author_sort | Peng, Ze |
collection | PubMed |
description | Most species in Rosaceae usually need to undergo several years of juvenile phase before the initiation of flowering. After 4–6 years’ juvenile phase, cultivated loquat (Eriobotrya japonica), a species in Rosaceae, enters the reproductive phase, blooms in the autumn and sets fruits during the winter. However, the mechanisms of the transition from a seedling to an adult tree remain obscure in loquat. The regulation networks controlling seasonal flowering are also largely unknown. Here, we report two RELATED TO ABI3 AND VP1 (RAV) homologs controlling juvenility and seasonal flowering in loquat. The expressions of EjRAV1/2 were relatively high during the juvenile or vegetative phase and low at the adult or reproductive phase. Overexpression of the two EjRAVs in Arabidopsis prolonged (about threefold) the juvenile period by repressing the expressions of flowering activator genes. Additionally, the transformed plants produced more lateral branches than the wild type plants. Molecular assays revealed that the nucleus localized EjRAVs could bind to the CAACA motif of the promoters of flower signal integrators, EjFT1/2, to repress their expression levels. These findings suggest that EjRAVs play critical roles in maintaining juvenility and repressing flower initiation in the early life cycle of loquat as well as in regulating seasonal flowering. Results from this study not only shed light on the control and maintenance of the juvenile phase, but also provided potential targets for manipulation of flowering time and accelerated breeding in loquat. |
format | Online Article Text |
id | pubmed-8759039 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-87590392022-01-15 EjRAV1/2 Delay Flowering Through Transcriptional Repression of EjFTs and EjSOC1s in Loquat Peng, Ze Wang, Man Zhang, Ling Jiang, Yuanyuan Zhao, Chongbin Shahid, Muhammad Qasim Bai, Yunlu Hao, Jingjing Peng, Jiangrong Gao, Yongshun Su, Wenbing Yang, Xianghui Front Plant Sci Plant Science Most species in Rosaceae usually need to undergo several years of juvenile phase before the initiation of flowering. After 4–6 years’ juvenile phase, cultivated loquat (Eriobotrya japonica), a species in Rosaceae, enters the reproductive phase, blooms in the autumn and sets fruits during the winter. However, the mechanisms of the transition from a seedling to an adult tree remain obscure in loquat. The regulation networks controlling seasonal flowering are also largely unknown. Here, we report two RELATED TO ABI3 AND VP1 (RAV) homologs controlling juvenility and seasonal flowering in loquat. The expressions of EjRAV1/2 were relatively high during the juvenile or vegetative phase and low at the adult or reproductive phase. Overexpression of the two EjRAVs in Arabidopsis prolonged (about threefold) the juvenile period by repressing the expressions of flowering activator genes. Additionally, the transformed plants produced more lateral branches than the wild type plants. Molecular assays revealed that the nucleus localized EjRAVs could bind to the CAACA motif of the promoters of flower signal integrators, EjFT1/2, to repress their expression levels. These findings suggest that EjRAVs play critical roles in maintaining juvenility and repressing flower initiation in the early life cycle of loquat as well as in regulating seasonal flowering. Results from this study not only shed light on the control and maintenance of the juvenile phase, but also provided potential targets for manipulation of flowering time and accelerated breeding in loquat. Frontiers Media S.A. 2021-12-31 /pmc/articles/PMC8759039/ /pubmed/35035390 http://dx.doi.org/10.3389/fpls.2021.816086 Text en Copyright © 2021 Peng, Wang, Zhang, Jiang, Zhao, Shahid, Bai, Hao, Peng, Gao, Su and Yang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Peng, Ze Wang, Man Zhang, Ling Jiang, Yuanyuan Zhao, Chongbin Shahid, Muhammad Qasim Bai, Yunlu Hao, Jingjing Peng, Jiangrong Gao, Yongshun Su, Wenbing Yang, Xianghui EjRAV1/2 Delay Flowering Through Transcriptional Repression of EjFTs and EjSOC1s in Loquat |
title | EjRAV1/2 Delay Flowering Through Transcriptional Repression of EjFTs and EjSOC1s in Loquat |
title_full | EjRAV1/2 Delay Flowering Through Transcriptional Repression of EjFTs and EjSOC1s in Loquat |
title_fullStr | EjRAV1/2 Delay Flowering Through Transcriptional Repression of EjFTs and EjSOC1s in Loquat |
title_full_unstemmed | EjRAV1/2 Delay Flowering Through Transcriptional Repression of EjFTs and EjSOC1s in Loquat |
title_short | EjRAV1/2 Delay Flowering Through Transcriptional Repression of EjFTs and EjSOC1s in Loquat |
title_sort | ejrav1/2 delay flowering through transcriptional repression of ejfts and ejsoc1s in loquat |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8759039/ https://www.ncbi.nlm.nih.gov/pubmed/35035390 http://dx.doi.org/10.3389/fpls.2021.816086 |
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