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Mapping Mechanostable Pulling Geometries of a Therapeutic Anticalin/CTLA-4 Protein Complex

[Image: see text] We used single-molecule AFM force spectroscopy (AFM-SMFS) in combination with click chemistry to mechanically dissociate anticalin, a non-antibody protein binding scaffold, from its target (CTLA-4), by pulling from eight different anchor residues. We found that pulling on the antic...

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Detalles Bibliográficos
Autores principales:	Liu, Zhaowei, Moreira, Rodrigo A., Dujmović, Ana, Liu, Haipei, Yang, Byeongseon, Poma, Adolfo B., Nash, Michael A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2021
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8759085/ https://www.ncbi.nlm.nih.gov/pubmed/34918516 http://dx.doi.org/10.1021/acs.nanolett.1c03584

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