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A subpopulation of astrocyte progenitors defined by Sonic hedgehog signaling

BACKGROUND: The molecular signaling pathway, Sonic hedgehog (Shh), is critical for the proper development of the central nervous system. The requirement for Shh signaling in neuronal and oligodendrocyte development in the developing embryo are well established. However, Shh activity is found in disc...

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Autores principales: Gingrich, Ellen C., Case, Kendra, Garcia, A. Denise R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8759290/
https://www.ncbi.nlm.nih.gov/pubmed/35027088
http://dx.doi.org/10.1186/s13064-021-00158-w
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author Gingrich, Ellen C.
Case, Kendra
Garcia, A. Denise R.
author_facet Gingrich, Ellen C.
Case, Kendra
Garcia, A. Denise R.
author_sort Gingrich, Ellen C.
collection PubMed
description BACKGROUND: The molecular signaling pathway, Sonic hedgehog (Shh), is critical for the proper development of the central nervous system. The requirement for Shh signaling in neuronal and oligodendrocyte development in the developing embryo are well established. However, Shh activity is found in discrete subpopulations of astrocytes in the postnatal and adult brain. Whether Shh signaling plays a role in astrocyte development is not well understood. METHODS: Here, we use a genetic inducible fate mapping approach to mark and follow a population of glial progenitor cells expressing the Shh target gene, Gli1, in the neonatal and postnatal brain. RESULTS: In the neonatal brain, Gli1-expressing cells are found in the dorsolateral corner of the subventricular zone (SVZ), a germinal zone harboring astrocyte progenitor cells. Our data show that these cells give rise to half of the cortical astrocyte population, demonstrating their substantial contribution to the cellular composition of the cortex. Further, these data suggest that the cortex harbors astrocytes from different lineages. Gli1 lineage astrocytes are distributed across all cortical layers, positioning them for broad influence over cortical circuits. Finally, we show that Shh activity recurs in mature astrocytes in a lineage-independent manner, suggesting cell-type dependent roles of the pathway in driving astrocyte development and function. CONCLUSION: These data identify a novel role for Shh signaling in cortical astrocyte development and support a growing body of evidence pointing to astrocyte heterogeneity. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13064-021-00158-w.
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spelling pubmed-87592902022-01-18 A subpopulation of astrocyte progenitors defined by Sonic hedgehog signaling Gingrich, Ellen C. Case, Kendra Garcia, A. Denise R. Neural Dev Research Article BACKGROUND: The molecular signaling pathway, Sonic hedgehog (Shh), is critical for the proper development of the central nervous system. The requirement for Shh signaling in neuronal and oligodendrocyte development in the developing embryo are well established. However, Shh activity is found in discrete subpopulations of astrocytes in the postnatal and adult brain. Whether Shh signaling plays a role in astrocyte development is not well understood. METHODS: Here, we use a genetic inducible fate mapping approach to mark and follow a population of glial progenitor cells expressing the Shh target gene, Gli1, in the neonatal and postnatal brain. RESULTS: In the neonatal brain, Gli1-expressing cells are found in the dorsolateral corner of the subventricular zone (SVZ), a germinal zone harboring astrocyte progenitor cells. Our data show that these cells give rise to half of the cortical astrocyte population, demonstrating their substantial contribution to the cellular composition of the cortex. Further, these data suggest that the cortex harbors astrocytes from different lineages. Gli1 lineage astrocytes are distributed across all cortical layers, positioning them for broad influence over cortical circuits. Finally, we show that Shh activity recurs in mature astrocytes in a lineage-independent manner, suggesting cell-type dependent roles of the pathway in driving astrocyte development and function. CONCLUSION: These data identify a novel role for Shh signaling in cortical astrocyte development and support a growing body of evidence pointing to astrocyte heterogeneity. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13064-021-00158-w. BioMed Central 2022-01-14 /pmc/articles/PMC8759290/ /pubmed/35027088 http://dx.doi.org/10.1186/s13064-021-00158-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Gingrich, Ellen C.
Case, Kendra
Garcia, A. Denise R.
A subpopulation of astrocyte progenitors defined by Sonic hedgehog signaling
title A subpopulation of astrocyte progenitors defined by Sonic hedgehog signaling
title_full A subpopulation of astrocyte progenitors defined by Sonic hedgehog signaling
title_fullStr A subpopulation of astrocyte progenitors defined by Sonic hedgehog signaling
title_full_unstemmed A subpopulation of astrocyte progenitors defined by Sonic hedgehog signaling
title_short A subpopulation of astrocyte progenitors defined by Sonic hedgehog signaling
title_sort subpopulation of astrocyte progenitors defined by sonic hedgehog signaling
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8759290/
https://www.ncbi.nlm.nih.gov/pubmed/35027088
http://dx.doi.org/10.1186/s13064-021-00158-w
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