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Viral dysbiosis in children with new-onset celiac disease

Viruses are common components of the intestinal microbiome, modulating host bacterial metabolism and interacting with the immune system, with a possible role in the pathogenesis of immune-mediated diseases such as celiac disease (CeD). The objective of this study was to characterize the virome profi...

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Autores principales: El Mouzan, Mohammad, Assiri, Asaad, Al Sarkhy, Ahmed, Alasmi, Mona, Saeed, Anjum, Al-Hussaini, Abdulrahman, AlSaleem, Badr, Al Mofarreh, Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8759644/
https://www.ncbi.nlm.nih.gov/pubmed/35030192
http://dx.doi.org/10.1371/journal.pone.0262108
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author El Mouzan, Mohammad
Assiri, Asaad
Al Sarkhy, Ahmed
Alasmi, Mona
Saeed, Anjum
Al-Hussaini, Abdulrahman
AlSaleem, Badr
Al Mofarreh, Mohammad
author_facet El Mouzan, Mohammad
Assiri, Asaad
Al Sarkhy, Ahmed
Alasmi, Mona
Saeed, Anjum
Al-Hussaini, Abdulrahman
AlSaleem, Badr
Al Mofarreh, Mohammad
author_sort El Mouzan, Mohammad
collection PubMed
description Viruses are common components of the intestinal microbiome, modulating host bacterial metabolism and interacting with the immune system, with a possible role in the pathogenesis of immune-mediated diseases such as celiac disease (CeD). The objective of this study was to characterize the virome profile in children with new-onset CeD. We used metagenomic analysis of viral DNA in mucosal and fecal samples from children with CeD and controls and performed sequencing using the Nextera XT library preparation kit. Abundance log2 fold changes were calculated using differential expression and linear discriminant effect size. Shannon alpha and Bray–Curtis beta diversity were determined. A total of 40 children with CeD and 39 controls were included. We found viral dysbiosis in both fecal and mucosal samples. Examples of significantly more abundant species in fecal samples of children with CeD included Human polyomavirus 2, Enterobacteria phage mEpX1, and Enterobacteria phage mEpX2; whereas less abundant species included Lactococcus phages ul36 and Streptococcus phage Abc2. In mucosal samples however, no species were significantly associated with CeD. Shannon alpha diversity was not significantly different between CeD and non-CeD groups and Bray–Curtis beta diversity showed no significant separation between CeD and non-CeD samples in either mucosal or stool samples, whereas separation was clear in all samples. We identified significant viral dysbiosis in children with CeD, suggesting a potential role in the pathogenesis of CeD indicating the need for further studies.
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spelling pubmed-87596442022-01-15 Viral dysbiosis in children with new-onset celiac disease El Mouzan, Mohammad Assiri, Asaad Al Sarkhy, Ahmed Alasmi, Mona Saeed, Anjum Al-Hussaini, Abdulrahman AlSaleem, Badr Al Mofarreh, Mohammad PLoS One Research Article Viruses are common components of the intestinal microbiome, modulating host bacterial metabolism and interacting with the immune system, with a possible role in the pathogenesis of immune-mediated diseases such as celiac disease (CeD). The objective of this study was to characterize the virome profile in children with new-onset CeD. We used metagenomic analysis of viral DNA in mucosal and fecal samples from children with CeD and controls and performed sequencing using the Nextera XT library preparation kit. Abundance log2 fold changes were calculated using differential expression and linear discriminant effect size. Shannon alpha and Bray–Curtis beta diversity were determined. A total of 40 children with CeD and 39 controls were included. We found viral dysbiosis in both fecal and mucosal samples. Examples of significantly more abundant species in fecal samples of children with CeD included Human polyomavirus 2, Enterobacteria phage mEpX1, and Enterobacteria phage mEpX2; whereas less abundant species included Lactococcus phages ul36 and Streptococcus phage Abc2. In mucosal samples however, no species were significantly associated with CeD. Shannon alpha diversity was not significantly different between CeD and non-CeD groups and Bray–Curtis beta diversity showed no significant separation between CeD and non-CeD samples in either mucosal or stool samples, whereas separation was clear in all samples. We identified significant viral dysbiosis in children with CeD, suggesting a potential role in the pathogenesis of CeD indicating the need for further studies. Public Library of Science 2022-01-14 /pmc/articles/PMC8759644/ /pubmed/35030192 http://dx.doi.org/10.1371/journal.pone.0262108 Text en © 2022 El Mouzan et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
El Mouzan, Mohammad
Assiri, Asaad
Al Sarkhy, Ahmed
Alasmi, Mona
Saeed, Anjum
Al-Hussaini, Abdulrahman
AlSaleem, Badr
Al Mofarreh, Mohammad
Viral dysbiosis in children with new-onset celiac disease
title Viral dysbiosis in children with new-onset celiac disease
title_full Viral dysbiosis in children with new-onset celiac disease
title_fullStr Viral dysbiosis in children with new-onset celiac disease
title_full_unstemmed Viral dysbiosis in children with new-onset celiac disease
title_short Viral dysbiosis in children with new-onset celiac disease
title_sort viral dysbiosis in children with new-onset celiac disease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8759644/
https://www.ncbi.nlm.nih.gov/pubmed/35030192
http://dx.doi.org/10.1371/journal.pone.0262108
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