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Detection of porcine enteric viruses (Kobuvirus, Mamastrovirus and Sapelovirus) in domestic pigs in Corsica, France

Many enteric viruses are found in pig farms around the world and can cause death of animals or important production losses for breeders. Among the wide spectrum of enteric viral species, porcine Sapelovirus (PSV), porcine Kobuvirus (PKoV) and porcine Astrovirus (PAstV) are frequently found in pig fe...

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Autores principales: Capai, Lisandru, Piorkowski, Géraldine, Maestrini, Oscar, Casabianca, François, Masse, Shirley, de Lamballerie, Xavier, Charrel, Rémi N., Falchi, Alessandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8759673/
https://www.ncbi.nlm.nih.gov/pubmed/35030164
http://dx.doi.org/10.1371/journal.pone.0260161
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author Capai, Lisandru
Piorkowski, Géraldine
Maestrini, Oscar
Casabianca, François
Masse, Shirley
de Lamballerie, Xavier
Charrel, Rémi N.
Falchi, Alessandra
author_facet Capai, Lisandru
Piorkowski, Géraldine
Maestrini, Oscar
Casabianca, François
Masse, Shirley
de Lamballerie, Xavier
Charrel, Rémi N.
Falchi, Alessandra
author_sort Capai, Lisandru
collection PubMed
description Many enteric viruses are found in pig farms around the world and can cause death of animals or important production losses for breeders. Among the wide spectrum of enteric viral species, porcine Sapelovirus (PSV), porcine Kobuvirus (PKoV) and porcine Astrovirus (PAstV) are frequently found in pig feces. In this study we investigated sixteen pig farms in Corsica, France, to evaluate the circulation of three enteric viruses (PKoV, PAstV-1 and PSV). In addition to the three viruses studied by RT–qPCR (908 pig feces samples), 26 stool samples were tested using the Next Generation Sequencing method (NGS). Our results showed viral RNA detection rates (i) of 62.0% [58.7–65.1] (n = 563/908) for PSV, (ii) of 44.8% [41.5–48.1] (n = 407/908) for PKoV and (iii) of 8.6% [6.8–10.6] (n = 78/908) for PAstV-1. Significant differences were observed for all three viruses according to age (P-value = 2.4e–13 for PAstV-1; 2.4e–12 for PKoV and 0.005 for PSV). The type of breeding was significantly associated with RNA detection only for PAstV-1 (P-value = 9.6e–6). Among the 26 samples tested with NGS method, consensus sequences corresponding to 10 different species of virus were detected. This study provides first insight on the presence of three common porcine enteric viruses in France. We also showed that they are frequently encountered in pigs born and bred in Corsica, which demonstrates endemic local circulation.
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spelling pubmed-87596732022-01-15 Detection of porcine enteric viruses (Kobuvirus, Mamastrovirus and Sapelovirus) in domestic pigs in Corsica, France Capai, Lisandru Piorkowski, Géraldine Maestrini, Oscar Casabianca, François Masse, Shirley de Lamballerie, Xavier Charrel, Rémi N. Falchi, Alessandra PLoS One Research Article Many enteric viruses are found in pig farms around the world and can cause death of animals or important production losses for breeders. Among the wide spectrum of enteric viral species, porcine Sapelovirus (PSV), porcine Kobuvirus (PKoV) and porcine Astrovirus (PAstV) are frequently found in pig feces. In this study we investigated sixteen pig farms in Corsica, France, to evaluate the circulation of three enteric viruses (PKoV, PAstV-1 and PSV). In addition to the three viruses studied by RT–qPCR (908 pig feces samples), 26 stool samples were tested using the Next Generation Sequencing method (NGS). Our results showed viral RNA detection rates (i) of 62.0% [58.7–65.1] (n = 563/908) for PSV, (ii) of 44.8% [41.5–48.1] (n = 407/908) for PKoV and (iii) of 8.6% [6.8–10.6] (n = 78/908) for PAstV-1. Significant differences were observed for all three viruses according to age (P-value = 2.4e–13 for PAstV-1; 2.4e–12 for PKoV and 0.005 for PSV). The type of breeding was significantly associated with RNA detection only for PAstV-1 (P-value = 9.6e–6). Among the 26 samples tested with NGS method, consensus sequences corresponding to 10 different species of virus were detected. This study provides first insight on the presence of three common porcine enteric viruses in France. We also showed that they are frequently encountered in pigs born and bred in Corsica, which demonstrates endemic local circulation. Public Library of Science 2022-01-14 /pmc/articles/PMC8759673/ /pubmed/35030164 http://dx.doi.org/10.1371/journal.pone.0260161 Text en © 2022 Capai et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Capai, Lisandru
Piorkowski, Géraldine
Maestrini, Oscar
Casabianca, François
Masse, Shirley
de Lamballerie, Xavier
Charrel, Rémi N.
Falchi, Alessandra
Detection of porcine enteric viruses (Kobuvirus, Mamastrovirus and Sapelovirus) in domestic pigs in Corsica, France
title Detection of porcine enteric viruses (Kobuvirus, Mamastrovirus and Sapelovirus) in domestic pigs in Corsica, France
title_full Detection of porcine enteric viruses (Kobuvirus, Mamastrovirus and Sapelovirus) in domestic pigs in Corsica, France
title_fullStr Detection of porcine enteric viruses (Kobuvirus, Mamastrovirus and Sapelovirus) in domestic pigs in Corsica, France
title_full_unstemmed Detection of porcine enteric viruses (Kobuvirus, Mamastrovirus and Sapelovirus) in domestic pigs in Corsica, France
title_short Detection of porcine enteric viruses (Kobuvirus, Mamastrovirus and Sapelovirus) in domestic pigs in Corsica, France
title_sort detection of porcine enteric viruses (kobuvirus, mamastrovirus and sapelovirus) in domestic pigs in corsica, france
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8759673/
https://www.ncbi.nlm.nih.gov/pubmed/35030164
http://dx.doi.org/10.1371/journal.pone.0260161
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