The Mitragyna speciosa (Kratom) Genome: a resource for data-mining potent pharmaceuticals that impact human health

Mitragyna speciosa (kratom) produces numerous compounds with pharmaceutical properties including the production of bioactive monoterpene indole and oxindole alkaloids. Using a linked-read approach, a 1,122,519,462 bp draft assembly of M. speciosa “Rifat” was generated with an N50 scaffold size of 1,...

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Autores principales: Brose, Julia, Lau, Kin H, Dang, Thu Thuy Thi, Hamilton, John P, Martins, Lívia do Vale, Hamberger, Britta, Hamberger, Bjoern, Jiang, Jiming, O’Connor, Sarah E, Buell, C Robin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Genome Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8759815/
https://www.ncbi.nlm.nih.gov/pubmed/33677570
http://dx.doi.org/10.1093/g3journal/jkab058
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author Brose, Julia
Lau, Kin H
Dang, Thu Thuy Thi
Hamilton, John P
Martins, Lívia do Vale
Hamberger, Britta
Hamberger, Bjoern
Jiang, Jiming
O’Connor, Sarah E
Buell, C Robin
author_facet Brose, Julia
Lau, Kin H
Dang, Thu Thuy Thi
Hamilton, John P
Martins, Lívia do Vale
Hamberger, Britta
Hamberger, Bjoern
Jiang, Jiming
O’Connor, Sarah E
Buell, C Robin
author_sort Brose, Julia
collection PubMed
description Mitragyna speciosa (kratom) produces numerous compounds with pharmaceutical properties including the production of bioactive monoterpene indole and oxindole alkaloids. Using a linked-read approach, a 1,122,519,462 bp draft assembly of M. speciosa “Rifat” was generated with an N50 scaffold size of 1,020,971 bp and an N50 contig size of 70,448 bp that encodes 55,746 genes. Chromosome counting revealed that “Rifat” is a tetraploid with a base chromosome number of 11, which was further corroborated by orthology and syntenic analysis of the genome. Analysis of genes and clusters involved in specialized metabolism revealed genes putatively involved in alkaloid biosynthesis. Access to the genome of M. speciosa will facilitate an improved understanding of alkaloid biosynthesis and accelerate the production of bioactive alkaloids in heterologous hosts.
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spelling pubmed-87598152022-01-18 The Mitragyna speciosa (Kratom) Genome: a resource for data-mining potent pharmaceuticals that impact human health Brose, Julia Lau, Kin H Dang, Thu Thuy Thi Hamilton, John P Martins, Lívia do Vale Hamberger, Britta Hamberger, Bjoern Jiang, Jiming O’Connor, Sarah E Buell, C Robin G3 (Bethesda) Genome Reports Mitragyna speciosa (kratom) produces numerous compounds with pharmaceutical properties including the production of bioactive monoterpene indole and oxindole alkaloids. Using a linked-read approach, a 1,122,519,462 bp draft assembly of M. speciosa “Rifat” was generated with an N50 scaffold size of 1,020,971 bp and an N50 contig size of 70,448 bp that encodes 55,746 genes. Chromosome counting revealed that “Rifat” is a tetraploid with a base chromosome number of 11, which was further corroborated by orthology and syntenic analysis of the genome. Analysis of genes and clusters involved in specialized metabolism revealed genes putatively involved in alkaloid biosynthesis. Access to the genome of M. speciosa will facilitate an improved understanding of alkaloid biosynthesis and accelerate the production of bioactive alkaloids in heterologous hosts. Oxford University Press 2021-03-02 /pmc/articles/PMC8759815/ /pubmed/33677570 http://dx.doi.org/10.1093/g3journal/jkab058 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Genome Reports
Brose, Julia
Lau, Kin H
Dang, Thu Thuy Thi
Hamilton, John P
Martins, Lívia do Vale
Hamberger, Britta
Hamberger, Bjoern
Jiang, Jiming
O’Connor, Sarah E
Buell, C Robin
The Mitragyna speciosa (Kratom) Genome: a resource for data-mining potent pharmaceuticals that impact human health
title The Mitragyna speciosa (Kratom) Genome: a resource for data-mining potent pharmaceuticals that impact human health
title_full The Mitragyna speciosa (Kratom) Genome: a resource for data-mining potent pharmaceuticals that impact human health
title_fullStr The Mitragyna speciosa (Kratom) Genome: a resource for data-mining potent pharmaceuticals that impact human health
title_full_unstemmed The Mitragyna speciosa (Kratom) Genome: a resource for data-mining potent pharmaceuticals that impact human health
title_short The Mitragyna speciosa (Kratom) Genome: a resource for data-mining potent pharmaceuticals that impact human health
title_sort mitragyna speciosa (kratom) genome: a resource for data-mining potent pharmaceuticals that impact human health
topic Genome Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8759815/
https://www.ncbi.nlm.nih.gov/pubmed/33677570
http://dx.doi.org/10.1093/g3journal/jkab058
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