Cargando…

MicroRNA-489 Promotes the Apoptosis of Cardiac Muscle Cells in Myocardial Ischemia-Reperfusion Based on Smart Healthcare

With the development of information technology, the concept of smart healthcare has gradually come to the fore. Smart healthcare uses a new generation of information technologies, such as the Internet of Things (loT), big data, cloud computing, and artificial intelligence, to transform the tradition...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Wenhua, Zhang, Yixin, Wang, Jian, Li, Qiang, Zhao, Di, Tang, Bozan, Wang, Shiwei, Shao, Haifeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8759872/
https://www.ncbi.nlm.nih.gov/pubmed/35035817
http://dx.doi.org/10.1155/2022/2538769
_version_ 1784633197585235968
author Li, Wenhua
Zhang, Yixin
Wang, Jian
Li, Qiang
Zhao, Di
Tang, Bozan
Wang, Shiwei
Shao, Haifeng
author_facet Li, Wenhua
Zhang, Yixin
Wang, Jian
Li, Qiang
Zhao, Di
Tang, Bozan
Wang, Shiwei
Shao, Haifeng
author_sort Li, Wenhua
collection PubMed
description With the development of information technology, the concept of smart healthcare has gradually come to the fore. Smart healthcare uses a new generation of information technologies, such as the Internet of Things (loT), big data, cloud computing, and artificial intelligence, to transform the traditional medical system in an all-around way, making healthcare more efficient, more convenient, and more personalized. miRNAs can regulate the proliferation, differentiation, and apoptosis of human cells. Relevant studies have also shown that miRNAs may play a key role in the occurrence and development of myocardial ischemia-reperfusion injury (MIRI). This study aims to explore the effects of miR-489 in MIRI. In this study, miR-489 expression in a myocardial ischemia-reperfusion animal model and H9C2 cells induced by H/R was detected by qRT-PCR. The release of lactate dehydrogenase (LDH) and the activity of creatine kinase (CK) was detected after miR-489 knockdown in H9C2 cells induced by H/R. The apoptosis of H9C2 cells and animal models were determined by ELISA. The relationship between miR-489 and SPIN1 was verified by a double fluorescence reporter enzyme assay. The expression of the PI3K/AKT pathway-related proteins was detected by Western blot. Experimental results showed that miR-489 was highly expressed in cardiac muscle cells of the animal model and in H9C2 cells induced by H/R of the myocardial infarction group, which was positively associated with the apoptosis of cardiac muscle cells with ischemia-reperfusion. miR-489 knockdown can reduce the apoptosis of cardiac muscle cells caused by ischemia-reperfusion. In downstream targeting studies, it was found that miR-489 promotes the apoptosis of cardiac muscle cells after ischemia-reperfusion by targeting the inhibition of the SPIN1-mediated PI3K/AKT pathway. In conclusion, high expression of miR-489 is associated with increased apoptosis of cardiac muscle cells after ischemia-reperfusion, which can promote the apoptosis after ischemia-reperfusion by targeting the inhibition of the SPIN1-mediated PI3K/AKT pathway. Therefore, miR-489 can be one of the potential therapeutic targets for reducing the apoptosis of cardiac muscle cells after ischemia-reperfusion.
format Online
Article
Text
id pubmed-8759872
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-87598722022-01-15 MicroRNA-489 Promotes the Apoptosis of Cardiac Muscle Cells in Myocardial Ischemia-Reperfusion Based on Smart Healthcare Li, Wenhua Zhang, Yixin Wang, Jian Li, Qiang Zhao, Di Tang, Bozan Wang, Shiwei Shao, Haifeng J Healthc Eng Research Article With the development of information technology, the concept of smart healthcare has gradually come to the fore. Smart healthcare uses a new generation of information technologies, such as the Internet of Things (loT), big data, cloud computing, and artificial intelligence, to transform the traditional medical system in an all-around way, making healthcare more efficient, more convenient, and more personalized. miRNAs can regulate the proliferation, differentiation, and apoptosis of human cells. Relevant studies have also shown that miRNAs may play a key role in the occurrence and development of myocardial ischemia-reperfusion injury (MIRI). This study aims to explore the effects of miR-489 in MIRI. In this study, miR-489 expression in a myocardial ischemia-reperfusion animal model and H9C2 cells induced by H/R was detected by qRT-PCR. The release of lactate dehydrogenase (LDH) and the activity of creatine kinase (CK) was detected after miR-489 knockdown in H9C2 cells induced by H/R. The apoptosis of H9C2 cells and animal models were determined by ELISA. The relationship between miR-489 and SPIN1 was verified by a double fluorescence reporter enzyme assay. The expression of the PI3K/AKT pathway-related proteins was detected by Western blot. Experimental results showed that miR-489 was highly expressed in cardiac muscle cells of the animal model and in H9C2 cells induced by H/R of the myocardial infarction group, which was positively associated with the apoptosis of cardiac muscle cells with ischemia-reperfusion. miR-489 knockdown can reduce the apoptosis of cardiac muscle cells caused by ischemia-reperfusion. In downstream targeting studies, it was found that miR-489 promotes the apoptosis of cardiac muscle cells after ischemia-reperfusion by targeting the inhibition of the SPIN1-mediated PI3K/AKT pathway. In conclusion, high expression of miR-489 is associated with increased apoptosis of cardiac muscle cells after ischemia-reperfusion, which can promote the apoptosis after ischemia-reperfusion by targeting the inhibition of the SPIN1-mediated PI3K/AKT pathway. Therefore, miR-489 can be one of the potential therapeutic targets for reducing the apoptosis of cardiac muscle cells after ischemia-reperfusion. Hindawi 2022-01-07 /pmc/articles/PMC8759872/ /pubmed/35035817 http://dx.doi.org/10.1155/2022/2538769 Text en Copyright © 2022 Wenhua Li et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Wenhua
Zhang, Yixin
Wang, Jian
Li, Qiang
Zhao, Di
Tang, Bozan
Wang, Shiwei
Shao, Haifeng
MicroRNA-489 Promotes the Apoptosis of Cardiac Muscle Cells in Myocardial Ischemia-Reperfusion Based on Smart Healthcare
title MicroRNA-489 Promotes the Apoptosis of Cardiac Muscle Cells in Myocardial Ischemia-Reperfusion Based on Smart Healthcare
title_full MicroRNA-489 Promotes the Apoptosis of Cardiac Muscle Cells in Myocardial Ischemia-Reperfusion Based on Smart Healthcare
title_fullStr MicroRNA-489 Promotes the Apoptosis of Cardiac Muscle Cells in Myocardial Ischemia-Reperfusion Based on Smart Healthcare
title_full_unstemmed MicroRNA-489 Promotes the Apoptosis of Cardiac Muscle Cells in Myocardial Ischemia-Reperfusion Based on Smart Healthcare
title_short MicroRNA-489 Promotes the Apoptosis of Cardiac Muscle Cells in Myocardial Ischemia-Reperfusion Based on Smart Healthcare
title_sort microrna-489 promotes the apoptosis of cardiac muscle cells in myocardial ischemia-reperfusion based on smart healthcare
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8759872/
https://www.ncbi.nlm.nih.gov/pubmed/35035817
http://dx.doi.org/10.1155/2022/2538769
work_keys_str_mv AT liwenhua microrna489promotestheapoptosisofcardiacmusclecellsinmyocardialischemiareperfusionbasedonsmarthealthcare
AT zhangyixin microrna489promotestheapoptosisofcardiacmusclecellsinmyocardialischemiareperfusionbasedonsmarthealthcare
AT wangjian microrna489promotestheapoptosisofcardiacmusclecellsinmyocardialischemiareperfusionbasedonsmarthealthcare
AT liqiang microrna489promotestheapoptosisofcardiacmusclecellsinmyocardialischemiareperfusionbasedonsmarthealthcare
AT zhaodi microrna489promotestheapoptosisofcardiacmusclecellsinmyocardialischemiareperfusionbasedonsmarthealthcare
AT tangbozan microrna489promotestheapoptosisofcardiacmusclecellsinmyocardialischemiareperfusionbasedonsmarthealthcare
AT wangshiwei microrna489promotestheapoptosisofcardiacmusclecellsinmyocardialischemiareperfusionbasedonsmarthealthcare
AT shaohaifeng microrna489promotestheapoptosisofcardiacmusclecellsinmyocardialischemiareperfusionbasedonsmarthealthcare