Low Cell Number Proteomic Analysis Using In-Cell Protease Digests Reveals a Robust Signature for Cell Cycle State Classification

Comprehensive proteome analysis of rare cell phenotypes remains a significant challenge. We report a method for low cell number MS-based proteomics using protease digestion of mildly formaldehyde-fixed cells in cellulo, which we call the “in-cell digest.” We combined this with averaged MS1 precursor...

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Detalles Bibliográficos
Autores principales: Kelly, Van, al-Rawi, Aymen, Lewis, David, Kustatscher, Georg, Ly, Tony
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2021
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8760417/
https://www.ncbi.nlm.nih.gov/pubmed/34742921
http://dx.doi.org/10.1016/j.mcpro.2021.100169

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8760417/
https://www.ncbi.nlm.nih.gov/pubmed/34742921
http://dx.doi.org/10.1016/j.mcpro.2021.100169

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