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Resolving the missing link between single platelet force and clot contractile force

Blood clot contraction plays an important role in wound healing and hemostasis. Although clot contraction is known to be driven by platelets, how single platelet forces relate to the forces generated by macroscopic clots remains largely unknown. Using our microfabricated high-throughput platelet con...

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Autores principales: Sun, Yueyi, Oshinowo, Oluwamayokun, Myers, David R., Lam, Wilbur A., Alexeev, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8760458/
https://www.ncbi.nlm.nih.gov/pubmed/35059605
http://dx.doi.org/10.1016/j.isci.2021.103690
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author Sun, Yueyi
Oshinowo, Oluwamayokun
Myers, David R.
Lam, Wilbur A.
Alexeev, Alexander
author_facet Sun, Yueyi
Oshinowo, Oluwamayokun
Myers, David R.
Lam, Wilbur A.
Alexeev, Alexander
author_sort Sun, Yueyi
collection PubMed
description Blood clot contraction plays an important role in wound healing and hemostasis. Although clot contraction is known to be driven by platelets, how single platelet forces relate to the forces generated by macroscopic clots remains largely unknown. Using our microfabricated high-throughput platelet contraction cytometer, we find that single platelets have an average force of 34 nN ([Formula: see text] healthy individuals). However, multiple bulk clot experiments predict a mean single platelet force lower than 0.5 nN. To resolve this discrepancy, we use a mesoscale computational model to probe the mechanism by which individual platelets induce forces in macroscopic clots. Our experimentally informed model shows that the number of platelets in the clot cross-section defines the net clot force. We provide a relationship between single platelet force and the clot force that is useful for better understanding of blood disorders associated with bleeding and thrombosis, and facilitates the development of platelet-based and platelet-mimetic biomaterials.
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spelling pubmed-87604582022-01-19 Resolving the missing link between single platelet force and clot contractile force Sun, Yueyi Oshinowo, Oluwamayokun Myers, David R. Lam, Wilbur A. Alexeev, Alexander iScience Article Blood clot contraction plays an important role in wound healing and hemostasis. Although clot contraction is known to be driven by platelets, how single platelet forces relate to the forces generated by macroscopic clots remains largely unknown. Using our microfabricated high-throughput platelet contraction cytometer, we find that single platelets have an average force of 34 nN ([Formula: see text] healthy individuals). However, multiple bulk clot experiments predict a mean single platelet force lower than 0.5 nN. To resolve this discrepancy, we use a mesoscale computational model to probe the mechanism by which individual platelets induce forces in macroscopic clots. Our experimentally informed model shows that the number of platelets in the clot cross-section defines the net clot force. We provide a relationship between single platelet force and the clot force that is useful for better understanding of blood disorders associated with bleeding and thrombosis, and facilitates the development of platelet-based and platelet-mimetic biomaterials. Elsevier 2021-12-27 /pmc/articles/PMC8760458/ /pubmed/35059605 http://dx.doi.org/10.1016/j.isci.2021.103690 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Sun, Yueyi
Oshinowo, Oluwamayokun
Myers, David R.
Lam, Wilbur A.
Alexeev, Alexander
Resolving the missing link between single platelet force and clot contractile force
title Resolving the missing link between single platelet force and clot contractile force
title_full Resolving the missing link between single platelet force and clot contractile force
title_fullStr Resolving the missing link between single platelet force and clot contractile force
title_full_unstemmed Resolving the missing link between single platelet force and clot contractile force
title_short Resolving the missing link between single platelet force and clot contractile force
title_sort resolving the missing link between single platelet force and clot contractile force
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8760458/
https://www.ncbi.nlm.nih.gov/pubmed/35059605
http://dx.doi.org/10.1016/j.isci.2021.103690
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