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Neural labeling and manipulation by neonatal intraventricular viral injection in mice

This step-by-step protocol provides a fast and easy technique to label and/or genetically manipulate neural cells, achieved by intraventricular injection of viral vectors into neonatal mice under ultrasound guidance. Successful injection of adeno-associated viral vectors (AAV) induces neural transdu...

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Detalles Bibliográficos
Autores principales: Wang, Mengzhe, Misgeld, Thomas, Brill, Monika S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8760487/
https://www.ncbi.nlm.nih.gov/pubmed/35059654
http://dx.doi.org/10.1016/j.xpro.2021.101081
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author Wang, Mengzhe
Misgeld, Thomas
Brill, Monika S.
author_facet Wang, Mengzhe
Misgeld, Thomas
Brill, Monika S.
author_sort Wang, Mengzhe
collection PubMed
description This step-by-step protocol provides a fast and easy technique to label and/or genetically manipulate neural cells, achieved by intraventricular injection of viral vectors into neonatal mice under ultrasound guidance. Successful injection of adeno-associated viral vectors (AAV) induces neural transduction as fast as 3 days post injection (dpi) in both the central and peripheral nervous systems. Virally driven expression persists until early adulthood. The same setup enables injection of other viral vectors as well as intramuscular injection. For complete details on the use and execution of this protocol, please refer to Wang et al. (2021) and Brill et al. (2016).
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spelling pubmed-87604872022-01-19 Neural labeling and manipulation by neonatal intraventricular viral injection in mice Wang, Mengzhe Misgeld, Thomas Brill, Monika S. STAR Protoc Protocol This step-by-step protocol provides a fast and easy technique to label and/or genetically manipulate neural cells, achieved by intraventricular injection of viral vectors into neonatal mice under ultrasound guidance. Successful injection of adeno-associated viral vectors (AAV) induces neural transduction as fast as 3 days post injection (dpi) in both the central and peripheral nervous systems. Virally driven expression persists until early adulthood. The same setup enables injection of other viral vectors as well as intramuscular injection. For complete details on the use and execution of this protocol, please refer to Wang et al. (2021) and Brill et al. (2016). Elsevier 2022-01-10 /pmc/articles/PMC8760487/ /pubmed/35059654 http://dx.doi.org/10.1016/j.xpro.2021.101081 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Wang, Mengzhe
Misgeld, Thomas
Brill, Monika S.
Neural labeling and manipulation by neonatal intraventricular viral injection in mice
title Neural labeling and manipulation by neonatal intraventricular viral injection in mice
title_full Neural labeling and manipulation by neonatal intraventricular viral injection in mice
title_fullStr Neural labeling and manipulation by neonatal intraventricular viral injection in mice
title_full_unstemmed Neural labeling and manipulation by neonatal intraventricular viral injection in mice
title_short Neural labeling and manipulation by neonatal intraventricular viral injection in mice
title_sort neural labeling and manipulation by neonatal intraventricular viral injection in mice
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8760487/
https://www.ncbi.nlm.nih.gov/pubmed/35059654
http://dx.doi.org/10.1016/j.xpro.2021.101081
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