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Neural labeling and manipulation by neonatal intraventricular viral injection in mice
This step-by-step protocol provides a fast and easy technique to label and/or genetically manipulate neural cells, achieved by intraventricular injection of viral vectors into neonatal mice under ultrasound guidance. Successful injection of adeno-associated viral vectors (AAV) induces neural transdu...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8760487/ https://www.ncbi.nlm.nih.gov/pubmed/35059654 http://dx.doi.org/10.1016/j.xpro.2021.101081 |
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author | Wang, Mengzhe Misgeld, Thomas Brill, Monika S. |
author_facet | Wang, Mengzhe Misgeld, Thomas Brill, Monika S. |
author_sort | Wang, Mengzhe |
collection | PubMed |
description | This step-by-step protocol provides a fast and easy technique to label and/or genetically manipulate neural cells, achieved by intraventricular injection of viral vectors into neonatal mice under ultrasound guidance. Successful injection of adeno-associated viral vectors (AAV) induces neural transduction as fast as 3 days post injection (dpi) in both the central and peripheral nervous systems. Virally driven expression persists until early adulthood. The same setup enables injection of other viral vectors as well as intramuscular injection. For complete details on the use and execution of this protocol, please refer to Wang et al. (2021) and Brill et al. (2016). |
format | Online Article Text |
id | pubmed-8760487 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-87604872022-01-19 Neural labeling and manipulation by neonatal intraventricular viral injection in mice Wang, Mengzhe Misgeld, Thomas Brill, Monika S. STAR Protoc Protocol This step-by-step protocol provides a fast and easy technique to label and/or genetically manipulate neural cells, achieved by intraventricular injection of viral vectors into neonatal mice under ultrasound guidance. Successful injection of adeno-associated viral vectors (AAV) induces neural transduction as fast as 3 days post injection (dpi) in both the central and peripheral nervous systems. Virally driven expression persists until early adulthood. The same setup enables injection of other viral vectors as well as intramuscular injection. For complete details on the use and execution of this protocol, please refer to Wang et al. (2021) and Brill et al. (2016). Elsevier 2022-01-10 /pmc/articles/PMC8760487/ /pubmed/35059654 http://dx.doi.org/10.1016/j.xpro.2021.101081 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Wang, Mengzhe Misgeld, Thomas Brill, Monika S. Neural labeling and manipulation by neonatal intraventricular viral injection in mice |
title | Neural labeling and manipulation by neonatal intraventricular viral injection in mice |
title_full | Neural labeling and manipulation by neonatal intraventricular viral injection in mice |
title_fullStr | Neural labeling and manipulation by neonatal intraventricular viral injection in mice |
title_full_unstemmed | Neural labeling and manipulation by neonatal intraventricular viral injection in mice |
title_short | Neural labeling and manipulation by neonatal intraventricular viral injection in mice |
title_sort | neural labeling and manipulation by neonatal intraventricular viral injection in mice |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8760487/ https://www.ncbi.nlm.nih.gov/pubmed/35059654 http://dx.doi.org/10.1016/j.xpro.2021.101081 |
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