m(6)A-label-seq: A metabolic labeling protocol to detect transcriptome-wide mRNA N(6)-methyladenosine (m(6)A) at base resolution

We describe here a metabolic labeling protocol for detecting cellular transcriptome-wide mRNA N(6)-methyladenosine (m(6)A) at base resolution. By feeding cells an analog of methionine, potential mRNA m(6)A forming sites are replaced with the N(6)-allyladenosine (a(6)A). A mild chemical iodination of...

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Detalles Bibliográficos
Autores principales: Shu, Xiao, Cao, Jie, Liu, Jianzhao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8760545/
https://www.ncbi.nlm.nih.gov/pubmed/35059657
http://dx.doi.org/10.1016/j.xpro.2021.101096

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8760545/
https://www.ncbi.nlm.nih.gov/pubmed/35059657
http://dx.doi.org/10.1016/j.xpro.2021.101096

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