Cargando…

m(6)A-label-seq: A metabolic labeling protocol to detect transcriptome-wide mRNA N(6)-methyladenosine (m(6)A) at base resolution

We describe here a metabolic labeling protocol for detecting cellular transcriptome-wide mRNA N(6)-methyladenosine (m(6)A) at base resolution. By feeding cells an analog of methionine, potential mRNA m(6)A forming sites are replaced with the N(6)-allyladenosine (a(6)A). A mild chemical iodination of...

Descripción completa

Detalles Bibliográficos
Autores principales:	Shu, Xiao, Cao, Jie, Liu, Jianzhao
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8760545/ https://www.ncbi.nlm.nih.gov/pubmed/35059657 http://dx.doi.org/10.1016/j.xpro.2021.101096

Ejemplares similares

Detection of m(6)A RNA modifications at single-nucleotide resolution using m(6)A-selective allyl chemical labeling and sequencing
por: Peng, Yong, et al.
Publicado: (2022)

The transcriptome-wide N6-methyladenosine (m(6)A) map profiling reveals the regulatory role of m(6)A in the yak ovary
por: Guo, Shaoke, et al.
Publicado: (2022)

Transcriptome-wide N6-methyladenosine (m(6)A) methylation in watermelon under CGMMV infection
por: He, Yanjun, et al.
Publicado: (2021)

Profiling of Transcriptome-Wide N6-Methyladenosine (m6A) Modifications and Identifying m6A Associated Regulation in Sperm Tail Formation in Anopheles sinensis
por: Liu, Congshan, et al.
Publicado: (2022)

Structural Insights into N(6)-methyladenosine (m(6)A) Modification in the Transcriptome
por: Huang, Jinbo, et al.
Publicado: (2018)

Widespread occurrence of N(6)-methyladenosine in bacterial mRNA
por: Deng, Xin, et al.
Publicado: (2015)

Roles and implications of mRNA N(6) ‐methyladenosine in cancer
por: Zeng, Lingxing, et al.
Publicado: (2023)

m6A-ELISA, a simple method for quantifying N6-methyladenosine from mRNA populations
por: Ensinck, Imke, et al.
Publicado: (2023)

MePMe-seq: antibody-free simultaneous m(6)A and m(5)C mapping in mRNA by metabolic propargyl labeling and sequencing
por: Hartstock, Katja, et al.
Publicado: (2023)

Genome-Wide Identification of N(6)-Methyladenosine (m(6)A) SNPs Associated With Rheumatoid Arthritis
por: Mo, Xing-Bo, et al.
Publicado: (2018)

Transcriptome-wide profiling of N(6)-methyladenosine via a selective chemical labeling method
por: Xie, Yalun, et al.
Publicado: (2022)

Regulation of Antiviral Immune Response by N(6)-Methyladenosine of mRNA
por: Zhao, Baoxin, et al.
Publicado: (2021)

mRNA N(6) ‐methyladenosine is critical for cold tolerance in Arabidopsis
por: Govindan, Ganesan, et al.
Publicado: (2022)

Genetic Analyses Support the Contribution of mRNA N(6)-methyladenosine (m(6)A) Modification to Human Disease Heritability
por: Zhang, Zijie, et al.
Publicado: (2020)

N(6)-methyladenosine modification-mediated mRNA metabolism is essential for human pancreatic lineage specification and islet organogenesis
por: Ma, Xiaojie, et al.
Publicado: (2022)

m6aViewer: software for the detection, analysis, and visualization of N(6)-methyladenosine peaks from m(6)A-seq/ME-RIP sequencing data
por: Antanaviciute, Agne, et al.
Publicado: (2017)

The Role of METTL3-Mediated N6-Methyladenosine (m6A) of JPH2 mRNA in Cyclophosphamide-Induced Cardiotoxicity
por: Zhu, Min, et al.
Publicado: (2021)

CSTF2 mediated mRNA N(6)-methyladenosine modification drives pancreatic ductal adenocarcinoma m(6)A subtypes
por: Zheng, Yanfen, et al.
Publicado: (2023)

Impact of N6-methyladenosine (m(6)A) modification on immunity
por: Elsabbagh, Raghda A., et al.
Publicado: (2022)

The role of N6-methyladenosine (m(6)A) in kidney diseases
por: You, Luling, et al.
Publicado: (2023)

m(6)A-Atlas: a comprehensive knowledgebase for unraveling the N(6)-methyladenosine (m(6)A) epitranscriptome
por: Tang, Yujiao, et al.
Publicado: (2020)

m(6)Am-seq reveals the dynamic m(6)Am methylation in the human transcriptome
por: Sun, Hanxiao, et al.
Publicado: (2021)

mRNA N(6)-methyladenosine methylation of postnatal liver development in pig
por: He, Shen, et al.
Publicado: (2017)

N6-methyladenosine mRNA methylation is important for the light response in soybean
por: Zhang, Liya, et al.
Publicado: (2023)

N(6)-methyladenosine modification of ITGA6 mRNA promotes the development and progression of bladder cancer
por: Jin, Huan, et al.
Publicado: (2019)

Single-nucleotide resolution mapping of m6A and m6Am throughout the transcriptome
por: Linder, Bastian, et al.
Publicado: (2015)

An m6A-Driven Prognostic Marker Panel for Renal Cell Carcinoma Based on the First Transcriptome-Wide m6A-seq
por: Waldbillig, Frank, et al.
Publicado: (2023)

m(6)A Reader: Epitranscriptome Target Prediction and Functional Characterization of N(6)-Methyladenosine (m(6)A) Readers
por: Zhen, Di, et al.
Publicado: (2020)

Detection of m(6)A in single cultured cells using scDART-seq
por: Tegowski, Matthew, et al.
Publicado: (2022)

N(6)‐methyladenosine (m(6)A) RNA modification in human cancer
por: Huo, Fu‐Chun, et al.
Publicado: (2020)

N(6)-methyladenosine (m(6)A) methylation in ischemia–reperfusion injury
por: Yao, Weifeng, et al.
Publicado: (2020)

Potential roles of N6-methyladenosine (m6A) in immune cells
por: Liu, Chang, et al.
Publicado: (2021)

N(6)-methyladenosine (m(6)A) RNA modification in tumor immunity
por: Zheng, Siyi, et al.
Publicado: (2022)

Regulatory Role of N6-methyladenosine (m(6)A) Modification in Osteosarcoma
por: Zhang, Yujie, et al.
Publicado: (2021)

Emerging Roles of N6-Methyladenosine (m6A) Epitranscriptomics in Toxicology
por: Malovic, Emir, et al.
Publicado: (2021)

Regulatory Role of N6-Methyladenosine (m6A) Modification in Osteoarthritis
por: Zhai, Ganggang, et al.
Publicado: (2022)

The implications of N6-methyladenosine (m6A) modification in esophageal carcinoma
por: He, Cheng, et al.
Publicado: (2023)

Interaction between N(6)-methyladenosine (m(6)A) modification and noncoding RNAs in cancer
por: Chen, Yi, et al.
Publicado: (2020)

The N6-Methyladenosine Features of mRNA and Aberrant Expression of m6A Modified Genes in Gastric Cancer and Their Potential Impact on the Risk and Prognosis
por: Sang, Liang, et al.
Publicado: (2020)

Systematic Investigation of mRNA N(6)-Methyladenosine Machinery in Primary Prostate Cancer
por: Jiang, Mei, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_r1uqih83io7pmf9vgnfk53h2lh