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MGcount: a total RNA-seq quantification tool to address multi-mapping and multi-overlapping alignments ambiguity in non-coding transcripts

BACKGROUND: Total-RNA sequencing (total-RNA-seq) allows the simultaneous study of both the coding and the non-coding transcriptome. Yet, computational pipelines have traditionally focused on particular biotypes, making assumptions that are not fullfilled by total-RNA-seq datasets. Transcripts from d...

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Detalles Bibliográficos
Autores principales: Hita, Andrea, Brocart, Gilles, Fernandez, Ana, Rehmsmeier, Marc, Alemany, Anna, Schvartzman, Sol
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8760670/
https://www.ncbi.nlm.nih.gov/pubmed/35030988
http://dx.doi.org/10.1186/s12859-021-04544-3