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MiR-937-3p promotes metastasis and angiogenesis and is activated by MYC in lung adenocarcinoma

BACKGROUND: Non-small cell lung cancer (NSCLC) is still one of the diseases with the highest mortality and morbidity, and lung adenocarcinoma (LUAD) accounts for more than half of all NSCLC cases in most countries. miRNA can be used as a potential biological marker and treatment for lung adenocarcin...

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Autores principales: Ma, Zijian, Chen, Ganyi, Chen, Yiqian, Guo, Zizhang, Chai, Hao, Tang, Yu, Zheng, Lin, Wei, Ke, Pan, Chunfeng, Ma, Zhifei, Xia, Yang, Zhang, Aiping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8761314/
https://www.ncbi.nlm.nih.gov/pubmed/35033084
http://dx.doi.org/10.1186/s12935-022-02453-w
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author Ma, Zijian
Chen, Ganyi
Chen, Yiqian
Guo, Zizhang
Chai, Hao
Tang, Yu
Zheng, Lin
Wei, Ke
Pan, Chunfeng
Ma, Zhifei
Xia, Yang
Zhang, Aiping
author_facet Ma, Zijian
Chen, Ganyi
Chen, Yiqian
Guo, Zizhang
Chai, Hao
Tang, Yu
Zheng, Lin
Wei, Ke
Pan, Chunfeng
Ma, Zhifei
Xia, Yang
Zhang, Aiping
author_sort Ma, Zijian
collection PubMed
description BACKGROUND: Non-small cell lung cancer (NSCLC) is still one of the diseases with the highest mortality and morbidity, and lung adenocarcinoma (LUAD) accounts for more than half of all NSCLC cases in most countries. miRNA can be used as a potential biological marker and treatment for lung adenocarcinoma. However, the effect of miR-937-3p to the invasion and metastasis of LUAD cells is not clear. METHODS: miRNA microarray is used to analyze the expression of miRNA in lung adenocarcinoma tissue. Transwell migration, Wound-healing assay and Western blot analysis are used to analyze cell migration, invasion and epithelial-mesenchymal transition (EMT) capabilities. Tube formation is used to assess angiogenesis ability. In addition, dual luciferase reporter gene detection is used to identify the potential binding between miRNA and target mRNA. In vivo experiments were performed on male NOD/SCID nude mice by tail vein injection to establish a transplanted tumor model. The CHIP experiment is used to verify the transcription factors of miRNA. RESULT: In our study, miR-937-3p was high-regulated in LUAD cell lines and tissues, and its expression level was related to tumor progression. We found that miR-937-3p high-expression has an effect on cell invasion and metastasis. In molecular mechanism, miR-937-3p causes SOX11 reduction by directly binding to the 3′-UTR of SOX11.In addition, MYC affects miR-937-3p transcription by binding to its promoter region. CONCLUSIONS: Our research shows that miR-937-3p is mediated by MYC and can control the angiogenesis, invasion and metastasis of LUAD by regulating SOX11, thereby promoting the progress of LUAD. We speculate that miR-937-3p can be used as a therapeutic target and potential biomarker for LUAD. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12935-022-02453-w.
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spelling pubmed-87613142022-01-18 MiR-937-3p promotes metastasis and angiogenesis and is activated by MYC in lung adenocarcinoma Ma, Zijian Chen, Ganyi Chen, Yiqian Guo, Zizhang Chai, Hao Tang, Yu Zheng, Lin Wei, Ke Pan, Chunfeng Ma, Zhifei Xia, Yang Zhang, Aiping Cancer Cell Int Primary Research BACKGROUND: Non-small cell lung cancer (NSCLC) is still one of the diseases with the highest mortality and morbidity, and lung adenocarcinoma (LUAD) accounts for more than half of all NSCLC cases in most countries. miRNA can be used as a potential biological marker and treatment for lung adenocarcinoma. However, the effect of miR-937-3p to the invasion and metastasis of LUAD cells is not clear. METHODS: miRNA microarray is used to analyze the expression of miRNA in lung adenocarcinoma tissue. Transwell migration, Wound-healing assay and Western blot analysis are used to analyze cell migration, invasion and epithelial-mesenchymal transition (EMT) capabilities. Tube formation is used to assess angiogenesis ability. In addition, dual luciferase reporter gene detection is used to identify the potential binding between miRNA and target mRNA. In vivo experiments were performed on male NOD/SCID nude mice by tail vein injection to establish a transplanted tumor model. The CHIP experiment is used to verify the transcription factors of miRNA. RESULT: In our study, miR-937-3p was high-regulated in LUAD cell lines and tissues, and its expression level was related to tumor progression. We found that miR-937-3p high-expression has an effect on cell invasion and metastasis. In molecular mechanism, miR-937-3p causes SOX11 reduction by directly binding to the 3′-UTR of SOX11.In addition, MYC affects miR-937-3p transcription by binding to its promoter region. CONCLUSIONS: Our research shows that miR-937-3p is mediated by MYC and can control the angiogenesis, invasion and metastasis of LUAD by regulating SOX11, thereby promoting the progress of LUAD. We speculate that miR-937-3p can be used as a therapeutic target and potential biomarker for LUAD. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12935-022-02453-w. BioMed Central 2022-01-15 /pmc/articles/PMC8761314/ /pubmed/35033084 http://dx.doi.org/10.1186/s12935-022-02453-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Primary Research
Ma, Zijian
Chen, Ganyi
Chen, Yiqian
Guo, Zizhang
Chai, Hao
Tang, Yu
Zheng, Lin
Wei, Ke
Pan, Chunfeng
Ma, Zhifei
Xia, Yang
Zhang, Aiping
MiR-937-3p promotes metastasis and angiogenesis and is activated by MYC in lung adenocarcinoma
title MiR-937-3p promotes metastasis and angiogenesis and is activated by MYC in lung adenocarcinoma
title_full MiR-937-3p promotes metastasis and angiogenesis and is activated by MYC in lung adenocarcinoma
title_fullStr MiR-937-3p promotes metastasis and angiogenesis and is activated by MYC in lung adenocarcinoma
title_full_unstemmed MiR-937-3p promotes metastasis and angiogenesis and is activated by MYC in lung adenocarcinoma
title_short MiR-937-3p promotes metastasis and angiogenesis and is activated by MYC in lung adenocarcinoma
title_sort mir-937-3p promotes metastasis and angiogenesis and is activated by myc in lung adenocarcinoma
topic Primary Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8761314/
https://www.ncbi.nlm.nih.gov/pubmed/35033084
http://dx.doi.org/10.1186/s12935-022-02453-w
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