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Effects of dexmedetomidine on the expression profile of tsRNAs in LPS‐induced acute lung injury

BACKGROUND: Acute lung injury (ALI) is characterized by impaired alveolar function and excessive inflammation, which is commonly seen in clinical anesthesia and intensive care units. tRNA‐derived small RNA (tsRNA) is a non‐coding RNA that can be used as a potential disease diagnostic biomarker. The...

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Autores principales: Lin, Yue, Cai, Junying, Huang, Dan, Zhou, Bin, Luo, Zhenzhong, Yu, Shuchun, Lu, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8761442/
https://www.ncbi.nlm.nih.gov/pubmed/34811808
http://dx.doi.org/10.1002/jcla.24115
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author Lin, Yue
Cai, Junying
Huang, Dan
Zhou, Bin
Luo, Zhenzhong
Yu, Shuchun
Lu, Jun
author_facet Lin, Yue
Cai, Junying
Huang, Dan
Zhou, Bin
Luo, Zhenzhong
Yu, Shuchun
Lu, Jun
author_sort Lin, Yue
collection PubMed
description BACKGROUND: Acute lung injury (ALI) is characterized by impaired alveolar function and excessive inflammation, which is commonly seen in clinical anesthesia and intensive care units. tRNA‐derived small RNA (tsRNA) is a non‐coding RNA that can be used as a potential disease diagnostic biomarker. The connection between ALI and tsRNA remains unknown. We aimed to explore the possible regulatory functions and mechanisms of tsRNAs in ALI treated with DEX. METHODS: Firstly, we established the ALI model by LPS injection and explored the effect of dexmedetomidine (DEX) treatment on lung damage. Then, the lung tissues were obtained from the LPS and LPS + DEX group for small RNA sequencing. RESULTS: We proved that DEX could ameliorate pulmonary injury, and decreased inflammation, pulmonary edema, and ferroptosis (MDA down‐regulation and GPX4 up‐regulation) in ALI. Furthermore, in the tsRNA expression profile, the top 10 down‐regulated tsRNAs were tsRNA‐1018, tsRNA‐3045b, tsRNA‐5021a, tsRNA‐1020, tsRNA‐5002b, tsRNA‐3045b, tsRNA‐1026, tsRNA‐5004a, tsRNA‐5005b and tsRNA‐1009, and the top 10 up‐regulated tsRNAs were tsRNA‐3025b, tsRNA‐3025a, tsRNA‐5016b, tsRNA‐3042b, tsRNA‐3029b, tsRNA‐3028b, tsRNA‐5006a, tsRNA‐3027b, tsRNA‐3027a, and tsRNA‐5009b. The enrichment analysis of GO terms and KEGG pathways pointed that target genes of DE‐tsRNAs were mainly enriched in regulation of transcription‐associated GO terms, NF‐kappa B signaling pathway, MAPK signaling pathway, and PI3K‐Akt signaling pathway. The RT‐qPCR results of tsRNA‐1020 and tsRNA‐1018 were in accordance with small RNA sequencing data. CONCLUSION: DEX affected the abnormal expression of tsRNAs in ALI. These aberrantly expressed tsRNAs and enriched physiological processes provide a scientific basis for the diagnosis and treatment of ALI.
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spelling pubmed-87614422022-01-20 Effects of dexmedetomidine on the expression profile of tsRNAs in LPS‐induced acute lung injury Lin, Yue Cai, Junying Huang, Dan Zhou, Bin Luo, Zhenzhong Yu, Shuchun Lu, Jun J Clin Lab Anal Research Articles BACKGROUND: Acute lung injury (ALI) is characterized by impaired alveolar function and excessive inflammation, which is commonly seen in clinical anesthesia and intensive care units. tRNA‐derived small RNA (tsRNA) is a non‐coding RNA that can be used as a potential disease diagnostic biomarker. The connection between ALI and tsRNA remains unknown. We aimed to explore the possible regulatory functions and mechanisms of tsRNAs in ALI treated with DEX. METHODS: Firstly, we established the ALI model by LPS injection and explored the effect of dexmedetomidine (DEX) treatment on lung damage. Then, the lung tissues were obtained from the LPS and LPS + DEX group for small RNA sequencing. RESULTS: We proved that DEX could ameliorate pulmonary injury, and decreased inflammation, pulmonary edema, and ferroptosis (MDA down‐regulation and GPX4 up‐regulation) in ALI. Furthermore, in the tsRNA expression profile, the top 10 down‐regulated tsRNAs were tsRNA‐1018, tsRNA‐3045b, tsRNA‐5021a, tsRNA‐1020, tsRNA‐5002b, tsRNA‐3045b, tsRNA‐1026, tsRNA‐5004a, tsRNA‐5005b and tsRNA‐1009, and the top 10 up‐regulated tsRNAs were tsRNA‐3025b, tsRNA‐3025a, tsRNA‐5016b, tsRNA‐3042b, tsRNA‐3029b, tsRNA‐3028b, tsRNA‐5006a, tsRNA‐3027b, tsRNA‐3027a, and tsRNA‐5009b. The enrichment analysis of GO terms and KEGG pathways pointed that target genes of DE‐tsRNAs were mainly enriched in regulation of transcription‐associated GO terms, NF‐kappa B signaling pathway, MAPK signaling pathway, and PI3K‐Akt signaling pathway. The RT‐qPCR results of tsRNA‐1020 and tsRNA‐1018 were in accordance with small RNA sequencing data. CONCLUSION: DEX affected the abnormal expression of tsRNAs in ALI. These aberrantly expressed tsRNAs and enriched physiological processes provide a scientific basis for the diagnosis and treatment of ALI. John Wiley and Sons Inc. 2021-11-22 /pmc/articles/PMC8761442/ /pubmed/34811808 http://dx.doi.org/10.1002/jcla.24115 Text en © 2021 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Lin, Yue
Cai, Junying
Huang, Dan
Zhou, Bin
Luo, Zhenzhong
Yu, Shuchun
Lu, Jun
Effects of dexmedetomidine on the expression profile of tsRNAs in LPS‐induced acute lung injury
title Effects of dexmedetomidine on the expression profile of tsRNAs in LPS‐induced acute lung injury
title_full Effects of dexmedetomidine on the expression profile of tsRNAs in LPS‐induced acute lung injury
title_fullStr Effects of dexmedetomidine on the expression profile of tsRNAs in LPS‐induced acute lung injury
title_full_unstemmed Effects of dexmedetomidine on the expression profile of tsRNAs in LPS‐induced acute lung injury
title_short Effects of dexmedetomidine on the expression profile of tsRNAs in LPS‐induced acute lung injury
title_sort effects of dexmedetomidine on the expression profile of tsrnas in lps‐induced acute lung injury
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8761442/
https://www.ncbi.nlm.nih.gov/pubmed/34811808
http://dx.doi.org/10.1002/jcla.24115
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