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The Rapid Antigen Detection Test for SARS-CoV-2 Underestimates the Identification of COVID-19 Positive Cases and Compromises the Diagnosis of the SARS-CoV-2 (K417N/T, E484K, and N501Y) Variants

Timely detection of severe acute respiratory syndrome due to coronavirus 2 (SARS-CoV-2) by reverse transcription quantitative polymerase chain reaction (RT-qPCR) has been the gold- strategy for identifying positive cases during the current pandemic. However, faster and less expensive methodologies a...

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Autores principales: Barrera-Avalos, Carlos, Luraschi, Roberto, Vallejos-Vidal, Eva, Mella-Torres, Andrea, Hernández, Felipe, Figueroa, Maximiliano, Rioseco, Claudia, Valdés, Daniel, Imarai, Mónica, Acuña-Castillo, Claudio, Reyes-López, Felipe E., Sandino, Ana María
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8761676/
https://www.ncbi.nlm.nih.gov/pubmed/35047474
http://dx.doi.org/10.3389/fpubh.2021.780801
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author Barrera-Avalos, Carlos
Luraschi, Roberto
Vallejos-Vidal, Eva
Mella-Torres, Andrea
Hernández, Felipe
Figueroa, Maximiliano
Rioseco, Claudia
Valdés, Daniel
Imarai, Mónica
Acuña-Castillo, Claudio
Reyes-López, Felipe E.
Sandino, Ana María
author_facet Barrera-Avalos, Carlos
Luraschi, Roberto
Vallejos-Vidal, Eva
Mella-Torres, Andrea
Hernández, Felipe
Figueroa, Maximiliano
Rioseco, Claudia
Valdés, Daniel
Imarai, Mónica
Acuña-Castillo, Claudio
Reyes-López, Felipe E.
Sandino, Ana María
author_sort Barrera-Avalos, Carlos
collection PubMed
description Timely detection of severe acute respiratory syndrome due to coronavirus 2 (SARS-CoV-2) by reverse transcription quantitative polymerase chain reaction (RT-qPCR) has been the gold- strategy for identifying positive cases during the current pandemic. However, faster and less expensive methodologies are also applied for the massive diagnosis of COVID-19. In this way, the rapid antigen test (RAT) is widely used. However, it is necessary to evaluate its detection efficiency considering the current pandemic context with the circulation of new viral variants. In this study, we evaluated the sensitivity and specificity of RAT (SD BIOSENSOR, South Korea), widely used for testing and SARS-CoV-2 diagnosis in Santiago of Chile. The RAT showed a 90% (amplification range of 20 ≤ Cq <25) and 10% (amplification range of 25 ≤ Cq <30) of positive SARS-CoV-2 cases identified previously by RT-qPCR. Importantly, a 0% detection was obtained for samples within a Cq value>30. In SARS-CoV-2 variant detection, RAT had a 42.8% detection sensitivity in samples with RT-qPCR amplification range 20 ≤ Cq <25 containing the single nucleotide polymorphisms (SNP) K417N/T, N501Y and E484K, associated with beta or gamma SARS-CoV-2 variants. This study alerts for the special attention that must be paid for the use of RAT at a massive diagnosis level, especially in the current scenario of appearance of several new SARS-CoV-2 variants which could generate false negatives and the compromise of possible viral outbreaks.
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spelling pubmed-87616762022-01-18 The Rapid Antigen Detection Test for SARS-CoV-2 Underestimates the Identification of COVID-19 Positive Cases and Compromises the Diagnosis of the SARS-CoV-2 (K417N/T, E484K, and N501Y) Variants Barrera-Avalos, Carlos Luraschi, Roberto Vallejos-Vidal, Eva Mella-Torres, Andrea Hernández, Felipe Figueroa, Maximiliano Rioseco, Claudia Valdés, Daniel Imarai, Mónica Acuña-Castillo, Claudio Reyes-López, Felipe E. Sandino, Ana María Front Public Health Public Health Timely detection of severe acute respiratory syndrome due to coronavirus 2 (SARS-CoV-2) by reverse transcription quantitative polymerase chain reaction (RT-qPCR) has been the gold- strategy for identifying positive cases during the current pandemic. However, faster and less expensive methodologies are also applied for the massive diagnosis of COVID-19. In this way, the rapid antigen test (RAT) is widely used. However, it is necessary to evaluate its detection efficiency considering the current pandemic context with the circulation of new viral variants. In this study, we evaluated the sensitivity and specificity of RAT (SD BIOSENSOR, South Korea), widely used for testing and SARS-CoV-2 diagnosis in Santiago of Chile. The RAT showed a 90% (amplification range of 20 ≤ Cq <25) and 10% (amplification range of 25 ≤ Cq <30) of positive SARS-CoV-2 cases identified previously by RT-qPCR. Importantly, a 0% detection was obtained for samples within a Cq value>30. In SARS-CoV-2 variant detection, RAT had a 42.8% detection sensitivity in samples with RT-qPCR amplification range 20 ≤ Cq <25 containing the single nucleotide polymorphisms (SNP) K417N/T, N501Y and E484K, associated with beta or gamma SARS-CoV-2 variants. This study alerts for the special attention that must be paid for the use of RAT at a massive diagnosis level, especially in the current scenario of appearance of several new SARS-CoV-2 variants which could generate false negatives and the compromise of possible viral outbreaks. Frontiers Media S.A. 2022-01-03 /pmc/articles/PMC8761676/ /pubmed/35047474 http://dx.doi.org/10.3389/fpubh.2021.780801 Text en Copyright © 2022 Barrera-Avalos, Luraschi, Vallejos-Vidal, Mella-Torres, Hernández, Figueroa, Rioseco, Valdés, Imarai, Acuña-Castillo, Reyes-López and Sandino. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Public Health
Barrera-Avalos, Carlos
Luraschi, Roberto
Vallejos-Vidal, Eva
Mella-Torres, Andrea
Hernández, Felipe
Figueroa, Maximiliano
Rioseco, Claudia
Valdés, Daniel
Imarai, Mónica
Acuña-Castillo, Claudio
Reyes-López, Felipe E.
Sandino, Ana María
The Rapid Antigen Detection Test for SARS-CoV-2 Underestimates the Identification of COVID-19 Positive Cases and Compromises the Diagnosis of the SARS-CoV-2 (K417N/T, E484K, and N501Y) Variants
title The Rapid Antigen Detection Test for SARS-CoV-2 Underestimates the Identification of COVID-19 Positive Cases and Compromises the Diagnosis of the SARS-CoV-2 (K417N/T, E484K, and N501Y) Variants
title_full The Rapid Antigen Detection Test for SARS-CoV-2 Underestimates the Identification of COVID-19 Positive Cases and Compromises the Diagnosis of the SARS-CoV-2 (K417N/T, E484K, and N501Y) Variants
title_fullStr The Rapid Antigen Detection Test for SARS-CoV-2 Underestimates the Identification of COVID-19 Positive Cases and Compromises the Diagnosis of the SARS-CoV-2 (K417N/T, E484K, and N501Y) Variants
title_full_unstemmed The Rapid Antigen Detection Test for SARS-CoV-2 Underestimates the Identification of COVID-19 Positive Cases and Compromises the Diagnosis of the SARS-CoV-2 (K417N/T, E484K, and N501Y) Variants
title_short The Rapid Antigen Detection Test for SARS-CoV-2 Underestimates the Identification of COVID-19 Positive Cases and Compromises the Diagnosis of the SARS-CoV-2 (K417N/T, E484K, and N501Y) Variants
title_sort rapid antigen detection test for sars-cov-2 underestimates the identification of covid-19 positive cases and compromises the diagnosis of the sars-cov-2 (k417n/t, e484k, and n501y) variants
topic Public Health
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8761676/
https://www.ncbi.nlm.nih.gov/pubmed/35047474
http://dx.doi.org/10.3389/fpubh.2021.780801
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