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RapidET: a MEMS-based platform for label-free and rapid demarcation of tumors from normal breast biopsy tissues
The rapid and label-free diagnosis of malignancies in ex vivo breast biopsy tissues has significant utility in pathology laboratories and operating rooms. We report a MEMS-based platform integrated with microchips that performs phenotyping of breast biopsy tissues using electrothermal sensing. The m...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8761751/ https://www.ncbi.nlm.nih.gov/pubmed/35087680 http://dx.doi.org/10.1038/s41378-021-00337-z |
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author | G. K., Anil Vishnu Gogoi, Gayatri Behera, Bhagaban Rila, Saeed Rangarajan, Annapoorni Pandya, Hardik J. |
author_facet | G. K., Anil Vishnu Gogoi, Gayatri Behera, Bhagaban Rila, Saeed Rangarajan, Annapoorni Pandya, Hardik J. |
author_sort | G. K., Anil Vishnu |
collection | PubMed |
description | The rapid and label-free diagnosis of malignancies in ex vivo breast biopsy tissues has significant utility in pathology laboratories and operating rooms. We report a MEMS-based platform integrated with microchips that performs phenotyping of breast biopsy tissues using electrothermal sensing. The microchip, fabricated on a silicon substrate, incorporates a platinum microheater, interdigitated electrodes (IDEs), and resistance temperature detectors (RTDs) as on-chip sensing elements. The microchips are integrated onto the platform using a slide-fit contact enabling quick replacement for biological measurements. The bulk resistivity (ρ(B)), surface resistivity (ρ(S)), and thermal conductivity (k) of deparaffinized and formalin-fixed paired tumor and adjacent normal breast biopsy samples from N = 8 patients were measured. For formalin-fixed samples, the mean ρ(B) for tumors showed a statistically significant fold change of 4.42 (P = 0.014) when the tissue was heated from 25 °C to 37 °C compared to the adjacent normal tissue, which showed a fold change of 3.47. The mean ρ(S) measurements also showed a similar trend. The mean k of the formalin-fixed tumor tissues was 0.309 ± 0.02 W m(−1) K(−1) compared to a significantly higher k of 0.563 ± 0.028 W m(−1) K(−1) for the adjacent normal tissues. A similar trend was observed in ρ(B,) ρ(S,) and k for the deparaffinized tissue samples. An analysis of a combination of ρ(B), ρ(S), and k using Fisher’s combined probability test and linear regression suggests the advantage of using all three parameters simultaneously for distinguishing tumors from adjacent normal tissues with higher statistical significance. |
format | Online Article Text |
id | pubmed-8761751 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-87617512022-01-26 RapidET: a MEMS-based platform for label-free and rapid demarcation of tumors from normal breast biopsy tissues G. K., Anil Vishnu Gogoi, Gayatri Behera, Bhagaban Rila, Saeed Rangarajan, Annapoorni Pandya, Hardik J. Microsyst Nanoeng Article The rapid and label-free diagnosis of malignancies in ex vivo breast biopsy tissues has significant utility in pathology laboratories and operating rooms. We report a MEMS-based platform integrated with microchips that performs phenotyping of breast biopsy tissues using electrothermal sensing. The microchip, fabricated on a silicon substrate, incorporates a platinum microheater, interdigitated electrodes (IDEs), and resistance temperature detectors (RTDs) as on-chip sensing elements. The microchips are integrated onto the platform using a slide-fit contact enabling quick replacement for biological measurements. The bulk resistivity (ρ(B)), surface resistivity (ρ(S)), and thermal conductivity (k) of deparaffinized and formalin-fixed paired tumor and adjacent normal breast biopsy samples from N = 8 patients were measured. For formalin-fixed samples, the mean ρ(B) for tumors showed a statistically significant fold change of 4.42 (P = 0.014) when the tissue was heated from 25 °C to 37 °C compared to the adjacent normal tissue, which showed a fold change of 3.47. The mean ρ(S) measurements also showed a similar trend. The mean k of the formalin-fixed tumor tissues was 0.309 ± 0.02 W m(−1) K(−1) compared to a significantly higher k of 0.563 ± 0.028 W m(−1) K(−1) for the adjacent normal tissues. A similar trend was observed in ρ(B,) ρ(S,) and k for the deparaffinized tissue samples. An analysis of a combination of ρ(B), ρ(S), and k using Fisher’s combined probability test and linear regression suggests the advantage of using all three parameters simultaneously for distinguishing tumors from adjacent normal tissues with higher statistical significance. Nature Publishing Group UK 2022-01-17 /pmc/articles/PMC8761751/ /pubmed/35087680 http://dx.doi.org/10.1038/s41378-021-00337-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article G. K., Anil Vishnu Gogoi, Gayatri Behera, Bhagaban Rila, Saeed Rangarajan, Annapoorni Pandya, Hardik J. RapidET: a MEMS-based platform for label-free and rapid demarcation of tumors from normal breast biopsy tissues |
title | RapidET: a MEMS-based platform for label-free and rapid demarcation of tumors from normal breast biopsy tissues |
title_full | RapidET: a MEMS-based platform for label-free and rapid demarcation of tumors from normal breast biopsy tissues |
title_fullStr | RapidET: a MEMS-based platform for label-free and rapid demarcation of tumors from normal breast biopsy tissues |
title_full_unstemmed | RapidET: a MEMS-based platform for label-free and rapid demarcation of tumors from normal breast biopsy tissues |
title_short | RapidET: a MEMS-based platform for label-free and rapid demarcation of tumors from normal breast biopsy tissues |
title_sort | rapidet: a mems-based platform for label-free and rapid demarcation of tumors from normal breast biopsy tissues |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8761751/ https://www.ncbi.nlm.nih.gov/pubmed/35087680 http://dx.doi.org/10.1038/s41378-021-00337-z |
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