Ribosome preparation from turquoise killifish skeletal muscle for cryo-EM

Ribosomes are composed of a mix of ribosomal RNAs and proteins; this composition varies depending on time, condition, and organism. Here, we present an optimized protocol for preparation of intact ribosomes from the skeletal muscle of the turquoise killifish. We also detail the steps for ribosome qu...

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Detalles Bibliográficos
Autores principales: Lee, Seongsin, Kim, Yumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8761774/
https://www.ncbi.nlm.nih.gov/pubmed/35072116
http://dx.doi.org/10.1016/j.xpro.2021.101087
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author Lee, Seongsin
Kim, Yumi
author_facet Lee, Seongsin
Kim, Yumi
author_sort Lee, Seongsin
collection PubMed
description Ribosomes are composed of a mix of ribosomal RNAs and proteins; this composition varies depending on time, condition, and organism. Here, we present an optimized protocol for preparation of intact ribosomes from the skeletal muscle of the turquoise killifish. We also detail the steps for ribosome quantification and cryo-EM grid preparation. This protocol can enable the identification of heterogeneous ribosome structures that vary by fish age or in response to specific conditions.
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spelling pubmed-87617742022-01-20 Ribosome preparation from turquoise killifish skeletal muscle for cryo-EM Lee, Seongsin Kim, Yumi STAR Protoc Protocol Ribosomes are composed of a mix of ribosomal RNAs and proteins; this composition varies depending on time, condition, and organism. Here, we present an optimized protocol for preparation of intact ribosomes from the skeletal muscle of the turquoise killifish. We also detail the steps for ribosome quantification and cryo-EM grid preparation. This protocol can enable the identification of heterogeneous ribosome structures that vary by fish age or in response to specific conditions. Elsevier 2022-01-12 /pmc/articles/PMC8761774/ /pubmed/35072116 http://dx.doi.org/10.1016/j.xpro.2021.101087 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Lee, Seongsin
Kim, Yumi
Ribosome preparation from turquoise killifish skeletal muscle for cryo-EM
title Ribosome preparation from turquoise killifish skeletal muscle for cryo-EM
title_full Ribosome preparation from turquoise killifish skeletal muscle for cryo-EM
title_fullStr Ribosome preparation from turquoise killifish skeletal muscle for cryo-EM
title_full_unstemmed Ribosome preparation from turquoise killifish skeletal muscle for cryo-EM
title_short Ribosome preparation from turquoise killifish skeletal muscle for cryo-EM
title_sort ribosome preparation from turquoise killifish skeletal muscle for cryo-em
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8761774/
https://www.ncbi.nlm.nih.gov/pubmed/35072116
http://dx.doi.org/10.1016/j.xpro.2021.101087
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