Cargando…
A protocol to investigate cellular and circuit mechanisms generating sharp wave ripple oscillations in rodent basolateral amygdala using ex vivo slices
Basolateral amygdala circuits generate oscillatory network activity to process and remember emotion-tagged events. Ex vivo preparations that recapitulate network activities seen in vivo provide an ideal system to investigate the mechanisms driving these network oscillations. Here we describe an ex v...
| Autores principales: | , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8761775/ https://www.ncbi.nlm.nih.gov/pubmed/35072114 http://dx.doi.org/10.1016/j.xpro.2021.101085 |
| Sumario: | Basolateral amygdala circuits generate oscillatory network activity to process and remember emotion-tagged events. Ex vivo preparations that recapitulate network activities seen in vivo provide an ideal system to investigate the mechanisms driving these network oscillations. Here we describe an ex vivo preparation of basolateral amygdala slices from rodents for measuring the generated sharp wave ripple oscillations (SWs) using local field potential recording and targeted recording from chandelier neurons that initiate SWs. For complete details on the use and execution of this protocol, please refer to Perumal et al. (2021). |
|---|